Tags

Type your tag names separated by a space and hit enter

Protective effects of increasing vitamin E and a doses on cisplatin-induced oxidative damage to kidney tissue in rats.
Urol Int. 2005; 75(4):340-4.UI

Abstract

OBJECTIVE

Cisplatin (DDP, cis-diamminedichloroplatinium II) is one of the most potent chemotherapeutic antitumor drugs, but is able to generate reactive oxygen species (ROS) and it also inhibits the activity of antioxidant enzymes in renal tissue. In the present study, we investigated the preventive effect of 100, 200 and 400 mg/kg b.w. doses of vitamin E (VE), and 25, 50, and 100 mg/kg b.w. doses of vitamin A (VA) combination on malondialdehyde (MDA), nitric oxide (NO), and glutathione (GSH) levels and superoxide dismutase (SOD) activity in cisplatin-induced toxicity in rat kidneys. Our literature survey indicated a lack of any experimental study showing the beneficial effect of VA on cisplatin-induced MDA, NO, GSH and SOD changes. For this reason, we hoped that this study would provide a unique contribution in that respect.

MATERIALS AND METHODS

59 Wistar rats (11 to replace prematurely lost animals) were used. 48 evaluable rats were divided into 8 groups (n = 6 in each group): control group, DDP alone (5 mg/kg b.w.) group, 3 VE combination treatment groups of VE100+DDP, VE200+DDP, and VE400+DDP, and 3 VA combination treatment groups of VA25+DDP, VA50+DDP, and VA100+DDP. Kidney MDA, GSH, NO levels and SOD activities were determined for the assessment of oxidant-antioxidant balance.

RESULTS

While in the DDP group the tissue levels of MDA and NO were found to be significantly higher than in the control group, GSH levels and SOD activities were significantly lower. MDA and NO levels were found to be significantly lower and GSH levels and SOD activities significantly higher in the VE200+DDP and VE400+ DDP groups when compared with the DDP alone group. MDA and NO levels were found to be significantly lower in the VA50+DDP and VA100+DDP groups when compared with the DDP alone group. However, identical comparisons with the DDP alone group showed significantly higher GSH levels and SOD activities in the VA25+DDP, VA50+DDP, and VA100+DDP groups. Among the VE100+ DDP, VE200+DDP, and VE400+DDP groups, and VA25+ DDP, VA50+DDP, and VA100+DDP groups, MDA and NO levels decreased and GSH levels and SOD activities increased steadily and significantly as the doses of VE and VA increased.

CONCLUSION

These vitamins would be effective in protecting against cisplatin-induced tissue damage in rat kidneys. It is possible that the toxic effect of cisplatin is somehow minimized by a compensatory mechanism involving VE and VA via induction of antioxidant enzyme activities following intraperitoneal injection of DDP.

Authors+Show Affiliations

Department of Medical Biochemistry, Kocaeli University, Faculty of Medicine, Kocaeli, Turkey. mozden@superonline.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16327303

Citation

Dillioglugil, Meltem Ozlen, et al. "Protective Effects of Increasing Vitamin E and a Doses On Cisplatin-induced Oxidative Damage to Kidney Tissue in Rats." Urologia Internationalis, vol. 75, no. 4, 2005, pp. 340-4.
Dillioglugil MO, Maral Kir H, Gulkac MD, et al. Protective effects of increasing vitamin E and a doses on cisplatin-induced oxidative damage to kidney tissue in rats. Urol Int. 2005;75(4):340-4.
Dillioglugil, M. O., Maral Kir, H., Gulkac, M. D., Ozon Kanli, A., Ozdogan, H. K., Acar, O., & Dillioglugil, O. (2005). Protective effects of increasing vitamin E and a doses on cisplatin-induced oxidative damage to kidney tissue in rats. Urologia Internationalis, 75(4), 340-4.
Dillioglugil MO, et al. Protective Effects of Increasing Vitamin E and a Doses On Cisplatin-induced Oxidative Damage to Kidney Tissue in Rats. Urol Int. 2005;75(4):340-4. PubMed PMID: 16327303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of increasing vitamin E and a doses on cisplatin-induced oxidative damage to kidney tissue in rats. AU - Dillioglugil,Meltem Ozlen, AU - Maral Kir,Hale, AU - Gulkac,Mehmet Dogan, AU - Ozon Kanli,Aylin, AU - Ozdogan,Haci Kahya, AU - Acar,Oguz, AU - Dillioglugil,Ozdal, PY - 2005/04/21/received PY - 2005/07/14/accepted PY - 2005/12/6/pubmed PY - 2006/2/10/medline PY - 2005/12/6/entrez SP - 340 EP - 4 JF - Urologia internationalis JO - Urol Int VL - 75 IS - 4 N2 - OBJECTIVE: Cisplatin (DDP, cis-diamminedichloroplatinium II) is one of the most potent chemotherapeutic antitumor drugs, but is able to generate reactive oxygen species (ROS) and it also inhibits the activity of antioxidant enzymes in renal tissue. In the present study, we investigated the preventive effect of 100, 200 and 400 mg/kg b.w. doses of vitamin E (VE), and 25, 50, and 100 mg/kg b.w. doses of vitamin A (VA) combination on malondialdehyde (MDA), nitric oxide (NO), and glutathione (GSH) levels and superoxide dismutase (SOD) activity in cisplatin-induced toxicity in rat kidneys. Our literature survey indicated a lack of any experimental study showing the beneficial effect of VA on cisplatin-induced MDA, NO, GSH and SOD changes. For this reason, we hoped that this study would provide a unique contribution in that respect. MATERIALS AND METHODS: 59 Wistar rats (11 to replace prematurely lost animals) were used. 48 evaluable rats were divided into 8 groups (n = 6 in each group): control group, DDP alone (5 mg/kg b.w.) group, 3 VE combination treatment groups of VE100+DDP, VE200+DDP, and VE400+DDP, and 3 VA combination treatment groups of VA25+DDP, VA50+DDP, and VA100+DDP. Kidney MDA, GSH, NO levels and SOD activities were determined for the assessment of oxidant-antioxidant balance. RESULTS: While in the DDP group the tissue levels of MDA and NO were found to be significantly higher than in the control group, GSH levels and SOD activities were significantly lower. MDA and NO levels were found to be significantly lower and GSH levels and SOD activities significantly higher in the VE200+DDP and VE400+ DDP groups when compared with the DDP alone group. MDA and NO levels were found to be significantly lower in the VA50+DDP and VA100+DDP groups when compared with the DDP alone group. However, identical comparisons with the DDP alone group showed significantly higher GSH levels and SOD activities in the VA25+DDP, VA50+DDP, and VA100+DDP groups. Among the VE100+ DDP, VE200+DDP, and VE400+DDP groups, and VA25+ DDP, VA50+DDP, and VA100+DDP groups, MDA and NO levels decreased and GSH levels and SOD activities increased steadily and significantly as the doses of VE and VA increased. CONCLUSION: These vitamins would be effective in protecting against cisplatin-induced tissue damage in rat kidneys. It is possible that the toxic effect of cisplatin is somehow minimized by a compensatory mechanism involving VE and VA via induction of antioxidant enzyme activities following intraperitoneal injection of DDP. SN - 0042-1138 UR - https://www.unboundmedicine.com/medline/citation/16327303/Protective_effects_of_increasing_vitamin_E_and_a_doses_on_cisplatin_induced_oxidative_damage_to_kidney_tissue_in_rats_ L2 - https://www.karger.com?DOI=10.1159/000089171 DB - PRIME DP - Unbound Medicine ER -