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A homozygous diploid subset of commercial wine yeast strains.
Antonie Van Leeuwenhoek. 2006 Jan; 89(1):27-37.AV

Abstract

Genetic analysis was performed on 45 commercial yeasts which are used in winemaking because of their superior fermentation properties. Genome sizes were estimated by propidium iodide fluorescence and flow cytometry. Forty strains had genome sizes consistent with their being diploid, while five had a range of aneuploid genome sizes that ranged from 1.2 to 1.8 times larger. The diploid strains are all Saccharomyces cerevisiae, based on genetic analysis of microsatellite and minisatellite markers and on DNA sequence analysis of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA of four strains. Four of the five aneuploid strains appeared to be interspecific hybrids between Saccharomyces kudriavzevii and Saccharomyces cerevisiae, with the fifth a hybrid between two S. cerevisiae strains. An identification fingerprint was constructed for the commercial yeast strains using 17 molecular markers. These included six published trinucleotide microsatellites, seven new dinucleotide microsatellites, and four published minisatellite markers. The markers provided unambiguous identification of the majority of strains; however, several had identical or similar patterns, and likely represent the same strain or mutants derived from it. The combined use of all 17 polymorphic loci allowed us to identify a set of eleven commercial wine yeast strains that appear to be genetically homozygous. These strains are presumed to have undergone inbreeding to maintain their homozygosity, a process referred to previously as 'genome renewal'.

Authors+Show Affiliations

School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16328862

Citation

Bradbury, John E., et al. "A Homozygous Diploid Subset of Commercial Wine Yeast Strains." Antonie Van Leeuwenhoek, vol. 89, no. 1, 2006, pp. 27-37.
Bradbury JE, Richards KD, Niederer HA, et al. A homozygous diploid subset of commercial wine yeast strains. Antonie Van Leeuwenhoek. 2006;89(1):27-37.
Bradbury, J. E., Richards, K. D., Niederer, H. A., Lee, S. A., Rod Dunbar, P., & Gardner, R. C. (2006). A homozygous diploid subset of commercial wine yeast strains. Antonie Van Leeuwenhoek, 89(1), 27-37.
Bradbury JE, et al. A Homozygous Diploid Subset of Commercial Wine Yeast Strains. Antonie Van Leeuwenhoek. 2006;89(1):27-37. PubMed PMID: 16328862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A homozygous diploid subset of commercial wine yeast strains. AU - Bradbury,John E, AU - Richards,Keith D, AU - Niederer,Heather A, AU - Lee,Soon A, AU - Rod Dunbar,P, AU - Gardner,Richard C, Y1 - 2005/12/03/ PY - 2005/04/06/received PY - 2005/08/04/accepted PY - 2005/12/6/pubmed PY - 2006/8/12/medline PY - 2005/12/6/entrez SP - 27 EP - 37 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 89 IS - 1 N2 - Genetic analysis was performed on 45 commercial yeasts which are used in winemaking because of their superior fermentation properties. Genome sizes were estimated by propidium iodide fluorescence and flow cytometry. Forty strains had genome sizes consistent with their being diploid, while five had a range of aneuploid genome sizes that ranged from 1.2 to 1.8 times larger. The diploid strains are all Saccharomyces cerevisiae, based on genetic analysis of microsatellite and minisatellite markers and on DNA sequence analysis of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA of four strains. Four of the five aneuploid strains appeared to be interspecific hybrids between Saccharomyces kudriavzevii and Saccharomyces cerevisiae, with the fifth a hybrid between two S. cerevisiae strains. An identification fingerprint was constructed for the commercial yeast strains using 17 molecular markers. These included six published trinucleotide microsatellites, seven new dinucleotide microsatellites, and four published minisatellite markers. The markers provided unambiguous identification of the majority of strains; however, several had identical or similar patterns, and likely represent the same strain or mutants derived from it. The combined use of all 17 polymorphic loci allowed us to identify a set of eleven commercial wine yeast strains that appear to be genetically homozygous. These strains are presumed to have undergone inbreeding to maintain their homozygosity, a process referred to previously as 'genome renewal'. SN - 0003-6072 UR - https://www.unboundmedicine.com/medline/citation/16328862/A_homozygous_diploid_subset_of_commercial_wine_yeast_strains_ DB - PRIME DP - Unbound Medicine ER -