Tags

Type your tag names separated by a space and hit enter

Antigenic cell associated dengue 2 virus proteins detected in vitro using dengue fever patients sera.
Malays J Pathol. 2002 Jun; 24(1):29-36.MJ

Abstract

At least three major antigenic dengue 2 virus proteins were recognized by pooled dengue fever patients' sera in infected Aedes albopictus (C6/36) mosquito cells. Dengue virus envelope (E), premembrane (PrM) and non-structural protein 1 (NS 1) dimer were detected beginning on day 3 postinfection in both the cell membrane and cytosolic fractions. Using the patients' sera, the presence of antigenic intermediate core protein (C)-PrM and NS1-non-structural protein 2a (NS2a) in the cytoplasmic fraction of dengue 2 virus infected cells was revealed. The presence of a approximately 92 and approximately 84 kDa NS 1 dimer in the membrane (NS 1m) and cytosolic (NS 1c) fractions of C6/36 cells, respectively, was also recognized. Using individual patient's serum, it was further confirmed that all patients' sera contained antibodies that specifically recognized E, NS 1 and PrM present in the dengue 2 virus-infected cell membrane fractions, suggesting that these glycosylated virus proteins were the main antigenic proteins recognized in vivo. Detection of dengue 2 virus C antibody in some patients further suggested that C could be antigenic if presented in vivo.

Authors+Show Affiliations

Abubakar S
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. sazaly@ummc.edu.my
Azila A
No affiliation info available
Suzana M
No affiliation info available
Chang LY
No affiliation info available

MeSH

AedesAnimalsAntibodies, ViralAntigens, ViralCell Culture TechniquesCell FractionationCell MembraneCytoplasmCytosolDengueDengue VirusViral Core ProteinsViral Envelope ProteinsViral Nonstructural ProteinsViral Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16329553

Citation

Abubakar, Sazaly, et al. "Antigenic Cell Associated Dengue 2 Virus Proteins Detected in Vitro Using Dengue Fever Patients Sera." The Malaysian Journal of Pathology, vol. 24, no. 1, 2002, pp. 29-36.
Abubakar S, Azila A, Suzana M, et al. Antigenic cell associated dengue 2 virus proteins detected in vitro using dengue fever patients sera. Malays J Pathol. 2002;24(1):29-36.
Abubakar, S., Azila, A., Suzana, M., & Chang, L. Y. (2002). Antigenic cell associated dengue 2 virus proteins detected in vitro using dengue fever patients sera. The Malaysian Journal of Pathology, 24(1), 29-36.
Abubakar S, et al. Antigenic Cell Associated Dengue 2 Virus Proteins Detected in Vitro Using Dengue Fever Patients Sera. Malays J Pathol. 2002;24(1):29-36. PubMed PMID: 16329553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigenic cell associated dengue 2 virus proteins detected in vitro using dengue fever patients sera. AU - Abubakar,Sazaly, AU - Azila,Azmi, AU - Suzana,Misbah, AU - Chang,Li-Yen, PY - 2005/12/7/pubmed PY - 2005/12/24/medline PY - 2005/12/7/entrez SP - 29 EP - 36 JF - The Malaysian journal of pathology JO - Malays J Pathol VL - 24 IS - 1 N2 - At least three major antigenic dengue 2 virus proteins were recognized by pooled dengue fever patients' sera in infected Aedes albopictus (C6/36) mosquito cells. Dengue virus envelope (E), premembrane (PrM) and non-structural protein 1 (NS 1) dimer were detected beginning on day 3 postinfection in both the cell membrane and cytosolic fractions. Using the patients' sera, the presence of antigenic intermediate core protein (C)-PrM and NS1-non-structural protein 2a (NS2a) in the cytoplasmic fraction of dengue 2 virus infected cells was revealed. The presence of a approximately 92 and approximately 84 kDa NS 1 dimer in the membrane (NS 1m) and cytosolic (NS 1c) fractions of C6/36 cells, respectively, was also recognized. Using individual patient's serum, it was further confirmed that all patients' sera contained antibodies that specifically recognized E, NS 1 and PrM present in the dengue 2 virus-infected cell membrane fractions, suggesting that these glycosylated virus proteins were the main antigenic proteins recognized in vivo. Detection of dengue 2 virus C antibody in some patients further suggested that C could be antigenic if presented in vivo. SN - 0126-8635 UR - https://www.unboundmedicine.com/medline/citation/16329553/Antigenic_cell_associated_dengue_2_virus_proteins_detected_in_vitro_using_dengue_fever_patients_sera_ DB - PRIME DP - Unbound Medicine ER -