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[Method of determination of biogenic amines in wines by high-performance liquid chromatography with fluorescence detector and ultraviolet detector].
Wei Sheng Yan Jiu. 2005 Sep; 34(5):577-80.WS

Abstract

OBJECTIVE

A pre-column derivation reversed-phase high-performance liquid chromatography method was developed for the determination of biogenic amines (tryptamine, phenylethyamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine) in wines with fluorescecse detector (FLD) and ultraviolet detector (UVD).

METHODS

Liqiud-liqiud extraction (LLE) was used to clean up wine samples with chloroform/butanol prior to derivatization with dansyl chloride, biogenic amines were separated using gradient elution. Mobile phase A was methanol and mobile phase B was water with the flow rate 1.5 ml/min. The column used was a CAPCELL PAK C18 MG (4.6 mm I.D. x 150 mm, 5 microm) and gradient elution at constant column tempereture with 30 degrees C under fluorescence detector with Ex 350nm and Em 520nm, The method and ultraviolet detector with 254nm. 1,7-Diaminoheptane was used as the internal standard (IS).

RESULTS

was linear for the amines studied at concentration ranging from 0.05 to 25 mg/L, except for spermidine and spermine, which ranging from 0.05 to 15 mg/L. The average recoveries ranged from 79.2% to 127.5% for all amines, The RSDs were less than 10%. With this method, 5 wine samples were determined, the results were favorable.

CONCLUSION

method can be applied for determination of biogenic amines in wines.

Authors+Show Affiliations

Department of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16329601

Citation

Li, Zhi-Jun, et al. "[Method of Determination of Biogenic Amines in Wines By High-performance Liquid Chromatography With Fluorescence Detector and Ultraviolet Detector]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 34, no. 5, 2005, pp. 577-80.
Li ZJ, Wu YN, Liu H, et al. [Method of determination of biogenic amines in wines by high-performance liquid chromatography with fluorescence detector and ultraviolet detector]. Wei Sheng Yan Jiu. 2005;34(5):577-80.
Li, Z. J., Wu, Y. N., Liu, H., & Xue, C. H. (2005). [Method of determination of biogenic amines in wines by high-performance liquid chromatography with fluorescence detector and ultraviolet detector]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 34(5), 577-80.
Li ZJ, et al. [Method of Determination of Biogenic Amines in Wines By High-performance Liquid Chromatography With Fluorescence Detector and Ultraviolet Detector]. Wei Sheng Yan Jiu. 2005;34(5):577-80. PubMed PMID: 16329601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Method of determination of biogenic amines in wines by high-performance liquid chromatography with fluorescence detector and ultraviolet detector]. AU - Li,Zhi-Jun, AU - Wu,Yong-Ning, AU - Liu,Hao, AU - Xue,Chang-Hu, PY - 2005/12/7/pubmed PY - 2010/7/30/medline PY - 2005/12/7/entrez SP - 577 EP - 80 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 34 IS - 5 N2 - OBJECTIVE: A pre-column derivation reversed-phase high-performance liquid chromatography method was developed for the determination of biogenic amines (tryptamine, phenylethyamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine) in wines with fluorescecse detector (FLD) and ultraviolet detector (UVD). METHODS: Liqiud-liqiud extraction (LLE) was used to clean up wine samples with chloroform/butanol prior to derivatization with dansyl chloride, biogenic amines were separated using gradient elution. Mobile phase A was methanol and mobile phase B was water with the flow rate 1.5 ml/min. The column used was a CAPCELL PAK C18 MG (4.6 mm I.D. x 150 mm, 5 microm) and gradient elution at constant column tempereture with 30 degrees C under fluorescence detector with Ex 350nm and Em 520nm, The method and ultraviolet detector with 254nm. 1,7-Diaminoheptane was used as the internal standard (IS). RESULTS: was linear for the amines studied at concentration ranging from 0.05 to 25 mg/L, except for spermidine and spermine, which ranging from 0.05 to 15 mg/L. The average recoveries ranged from 79.2% to 127.5% for all amines, The RSDs were less than 10%. With this method, 5 wine samples were determined, the results were favorable. CONCLUSION: method can be applied for determination of biogenic amines in wines. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/16329601/[Method_of_determination_of_biogenic_amines_in_wines_by_high_performance_liquid_chromatography_with_fluorescence_detector_and_ultraviolet_detector]_ DB - PRIME DP - Unbound Medicine ER -