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[Study on using in vitro 3T3 neutral red uptake to test phototoxicity].
Wei Sheng Yan Jiu 2005; 34(5):628-30WS

Abstract

OBJECTIVE

To establish neutral red uptake test of 3T3 in vitro to detect phototoxicity of chemicals and study the in vitro 3T3 NUR as an alternative method instead of animal skin phototoxicity test.

METHODS

Using incubated Balb/c3T3 cells to establish the optimal conditions of reaction system.

RESULTS

The concentration of neutral red, the incubate time, pH and the density of cells were the factors effecting test results.

CONCLUSION

It is suggested that in vitro 3T3 neutral red uptake test can be used as an alternative instead of animals phototoxicity test.

Authors+Show Affiliations

Institute for Environment and Product Related Health Safety, China Center for Disease Control and Prevention, Beijing 100021, China.No affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16329616

Citation

Zhang, Hong-Wei, and Shao-Xia Dong. "[Study On Using in Vitro 3T3 Neutral Red Uptake to Test Phototoxicity]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 34, no. 5, 2005, pp. 628-30.
Zhang HW, Dong SX. [Study on using in vitro 3T3 neutral red uptake to test phototoxicity]. Wei Sheng Yan Jiu. 2005;34(5):628-30.
Zhang, H. W., & Dong, S. X. (2005). [Study on using in vitro 3T3 neutral red uptake to test phototoxicity]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 34(5), pp. 628-30.
Zhang HW, Dong SX. [Study On Using in Vitro 3T3 Neutral Red Uptake to Test Phototoxicity]. Wei Sheng Yan Jiu. 2005;34(5):628-30. PubMed PMID: 16329616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on using in vitro 3T3 neutral red uptake to test phototoxicity]. AU - Zhang,Hong-Wei, AU - Dong,Shao-Xia, PY - 2005/12/7/pubmed PY - 2010/7/30/medline PY - 2005/12/7/entrez SP - 628 EP - 30 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 34 IS - 5 N2 - OBJECTIVE: To establish neutral red uptake test of 3T3 in vitro to detect phototoxicity of chemicals and study the in vitro 3T3 NUR as an alternative method instead of animal skin phototoxicity test. METHODS: Using incubated Balb/c3T3 cells to establish the optimal conditions of reaction system. RESULTS: The concentration of neutral red, the incubate time, pH and the density of cells were the factors effecting test results. CONCLUSION: It is suggested that in vitro 3T3 neutral red uptake test can be used as an alternative instead of animals phototoxicity test. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/16329616/[Study_on_using_in_vitro_3T3_neutral_red_uptake_to_test_phototoxicity]_ L2 - https://medlineplus.gov/cosmetics.html DB - PRIME DP - Unbound Medicine ER -