Tags

Type your tag names separated by a space and hit enter

TNF-alpha enhances estrogen-induced cell proliferation of estrogen-dependent breast tumor cells through a complex containing nuclear factor-kappa B.
Oncogene. 2006 Mar 02; 25(9):1367-77.O

Abstract

Breast tumors are usually classified according to their response to estrogens as hormone-dependent or -independent. In this work, we investigated the role of the proinflammatory cytokine TNF-alpha on the estrogen-receptor-positive T47D breast ductal tumor cells. We have found that TNF-alpha exerts a mitogenic effect, inducing cyclin D1 expression and activation of the transcription factor NF-kappaB. Importantly, activation of NF-kappaB was required for estrogen-induced proliferation and cyclin D1 expression. TNF-alpha enhanced the estrogen response by increasing the levels and availability of NF-kappaB. Chromatin immunoprecipitation analysis suggested that the action of estrogens is mediated by a protein complex that contains the activated estrogen receptor, the nuclear receptor coactivator RAC3 and a member of the NF-kappaB family. Finally, our results demonstrate that activation of this transcription factor could be one of the key signals for estrogen-mediated response.

Authors+Show Affiliations

Laboratorio de Biología Molecular y Apoptosis, Departamento de Sustancias Vasoactivas, Instituto de Investigaciones Médicas Alfredo Lanari, IDIM-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16331275

Citation

Rubio, M F., et al. "TNF-alpha Enhances Estrogen-induced Cell Proliferation of Estrogen-dependent Breast Tumor Cells Through a Complex Containing Nuclear Factor-kappa B." Oncogene, vol. 25, no. 9, 2006, pp. 1367-77.
Rubio MF, Werbajh S, Cafferata EG, et al. TNF-alpha enhances estrogen-induced cell proliferation of estrogen-dependent breast tumor cells through a complex containing nuclear factor-kappa B. Oncogene. 2006;25(9):1367-77.
Rubio, M. F., Werbajh, S., Cafferata, E. G., Quaglino, A., Coló, G. P., Nojek, I. M., Kordon, E. C., Nahmod, V. E., & Costas, M. A. (2006). TNF-alpha enhances estrogen-induced cell proliferation of estrogen-dependent breast tumor cells through a complex containing nuclear factor-kappa B. Oncogene, 25(9), 1367-77.
Rubio MF, et al. TNF-alpha Enhances Estrogen-induced Cell Proliferation of Estrogen-dependent Breast Tumor Cells Through a Complex Containing Nuclear Factor-kappa B. Oncogene. 2006 Mar 2;25(9):1367-77. PubMed PMID: 16331275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TNF-alpha enhances estrogen-induced cell proliferation of estrogen-dependent breast tumor cells through a complex containing nuclear factor-kappa B. AU - Rubio,M F, AU - Werbajh,S, AU - Cafferata,E G A, AU - Quaglino,A, AU - Coló,G P, AU - Nojek,I M, AU - Kordon,E C, AU - Nahmod,V E, AU - Costas,M A, PY - 2005/12/7/pubmed PY - 2006/4/19/medline PY - 2005/12/7/entrez SP - 1367 EP - 77 JF - Oncogene JO - Oncogene VL - 25 IS - 9 N2 - Breast tumors are usually classified according to their response to estrogens as hormone-dependent or -independent. In this work, we investigated the role of the proinflammatory cytokine TNF-alpha on the estrogen-receptor-positive T47D breast ductal tumor cells. We have found that TNF-alpha exerts a mitogenic effect, inducing cyclin D1 expression and activation of the transcription factor NF-kappaB. Importantly, activation of NF-kappaB was required for estrogen-induced proliferation and cyclin D1 expression. TNF-alpha enhanced the estrogen response by increasing the levels and availability of NF-kappaB. Chromatin immunoprecipitation analysis suggested that the action of estrogens is mediated by a protein complex that contains the activated estrogen receptor, the nuclear receptor coactivator RAC3 and a member of the NF-kappaB family. Finally, our results demonstrate that activation of this transcription factor could be one of the key signals for estrogen-mediated response. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/16331275/TNF_alpha_enhances_estrogen_induced_cell_proliferation_of_estrogen_dependent_breast_tumor_cells_through_a_complex_containing_nuclear_factor_kappa_B_ L2 - https://doi.org/10.1038/sj.onc.1209176 DB - PRIME DP - Unbound Medicine ER -