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Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis.
J Periodontol. 2005 Dec; 76(12):2223-9.JP

Abstract

BACKGROUND

Enamel matrix derivative (EMD) is a tissue regenerative agent used clinically as an adjunct to periodontal surgery. It was previously demonstrated that Porphyromonas gingivalis, a periodontal pathogen, significantly diminished the efficacy of EMD with periodontal ligament (PDL) cells through the proteolytic actions of Arg- and Lys-gingipains (Rgp and Kgp). Thus, antiproteolytic supplements are considered clinically desirable for effective periodontal regenerative therapies. In the present study, we examined apple- (AP) and hop-polyphenols to determine their ability to protect EMD-stimulated PDL cells from P. gingivalis.

METHODS

AP, apple condensed tannin (ACT), hop bract polyphenol (HBP), high and low molecular weight fractions of HBP (HMW-HBP and LMW-HBP), and epigallocatechin gallate (EGCg) were used. PDL cells were grown on EMD-coated dishes and infected with P. gingivalis, and cellular migration and proliferation were evaluated with an in vitro assay of wound healing assay in the presence or absence of the polyphenols.

RESULTS

Each polyphenol significantly enhanced the viability of PDL cells infected with P. gingivalis, whereas only EGCg demonstrated cytotoxicity. Further, all polyphenols significantly inhibited Rgp activity, with AP, ACT, and HBP more effective toward Kgp. P. gingivalis markedly diminished the migration and proliferation of EMD-stimulated PDL cells, whereas the addition of AP, ACT, HBP, and HMW-HBP significantly protected the cells from bacterial cytotoxicity. In contrast, EGCg and LMW-HBP did not show protective effects.

CONCLUSION

These results suggest that AP, ACT, AP, HBP, and HMW-HBP protect EMD-stimulated PDL cells from P. gingivalis and may be therapeutically useful supplements for EMD therapy.

Authors+Show Affiliations

Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16332233

Citation

Inaba, Hiroaki, et al. "Apple- and Hop-polyphenols Protect Periodontal Ligament Cells Stimulated With Enamel Matrix Derivative From Porphyromonas Gingivalis." Journal of Periodontology, vol. 76, no. 12, 2005, pp. 2223-9.
Inaba H, Tagashira M, Kanda T, et al. Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis. J Periodontol. 2005;76(12):2223-9.
Inaba, H., Tagashira, M., Kanda, T., Ohno, T., Kawai, S., & Amano, A. (2005). Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis. Journal of Periodontology, 76(12), 2223-9.
Inaba H, et al. Apple- and Hop-polyphenols Protect Periodontal Ligament Cells Stimulated With Enamel Matrix Derivative From Porphyromonas Gingivalis. J Periodontol. 2005;76(12):2223-9. PubMed PMID: 16332233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis. AU - Inaba,Hiroaki, AU - Tagashira,Motoyuki, AU - Kanda,Tomomasa, AU - Ohno,Takashi, AU - Kawai,Shinji, AU - Amano,Atsuo, PY - 2005/12/8/pubmed PY - 2006/3/24/medline PY - 2005/12/8/entrez SP - 2223 EP - 9 JF - Journal of periodontology JO - J Periodontol VL - 76 IS - 12 N2 - BACKGROUND: Enamel matrix derivative (EMD) is a tissue regenerative agent used clinically as an adjunct to periodontal surgery. It was previously demonstrated that Porphyromonas gingivalis, a periodontal pathogen, significantly diminished the efficacy of EMD with periodontal ligament (PDL) cells through the proteolytic actions of Arg- and Lys-gingipains (Rgp and Kgp). Thus, antiproteolytic supplements are considered clinically desirable for effective periodontal regenerative therapies. In the present study, we examined apple- (AP) and hop-polyphenols to determine their ability to protect EMD-stimulated PDL cells from P. gingivalis. METHODS: AP, apple condensed tannin (ACT), hop bract polyphenol (HBP), high and low molecular weight fractions of HBP (HMW-HBP and LMW-HBP), and epigallocatechin gallate (EGCg) were used. PDL cells were grown on EMD-coated dishes and infected with P. gingivalis, and cellular migration and proliferation were evaluated with an in vitro assay of wound healing assay in the presence or absence of the polyphenols. RESULTS: Each polyphenol significantly enhanced the viability of PDL cells infected with P. gingivalis, whereas only EGCg demonstrated cytotoxicity. Further, all polyphenols significantly inhibited Rgp activity, with AP, ACT, and HBP more effective toward Kgp. P. gingivalis markedly diminished the migration and proliferation of EMD-stimulated PDL cells, whereas the addition of AP, ACT, HBP, and HMW-HBP significantly protected the cells from bacterial cytotoxicity. In contrast, EGCg and LMW-HBP did not show protective effects. CONCLUSION: These results suggest that AP, ACT, AP, HBP, and HMW-HBP protect EMD-stimulated PDL cells from P. gingivalis and may be therapeutically useful supplements for EMD therapy. SN - 0022-3492 UR - https://www.unboundmedicine.com/medline/citation/16332233/Apple__and_hop_polyphenols_protect_periodontal_ligament_cells_stimulated_with_enamel_matrix_derivative_from_Porphyromonas_gingivalis_ L2 - https://doi.org/10.1902/jop.2005.76.12.2223 DB - PRIME DP - Unbound Medicine ER -