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Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder.
Am J Physiol Renal Physiol 2006; 290(5):F1143-52AJ

Abstract

The distribution and function of P2X and P2Y receptor subtypes were investigated on intact or cultured intramural ganglia of the cat urinary bladder by immunocytochemistry and calcium-imaging techniques, respectively. Neurons were labeled by all seven P2X receptor subtype antibodies and antibodies for P2Y(2), P2Y(4), P2Y(6), and P2Y(12) receptor subtypes with a staining intensity of immunoreactivity in the following order: P2X(3)=P2Y(2)=P2Y(4)=P2Y(6)=P2Y(12)>P2X(1)=P2X(2)=P2X(4)>P2X(5)=P2X(6)=P2X(7). P2Y(1) receptor antibodies labeled glial cells, but not neurons. P2X(3) and P2Y(4) polyclonal antibodies labeled approximately 95 and 40% of neurons, respectively. Double staining showed that 100, 48.8, and 97.4% of P2X(3) receptor-positive neurons coexpressed choline acetyl transferase (ChAT), nitric oxide synthase (NOS), and neurofilament 200 (NF200), respectively, whereas 100, 59.2, and 97.6% of P2Y(4) receptor-positive neurons coexpressed ChAT, NOS, and NF200, respectively. Application of ATP, alpha,beta-methylene ATP, and uridine triphosphate elevated intracellular Ca(2+) concentration in a subpopulation of dissociated cultured cat intramural ganglia neurons, demonstrating the presence of functional P2Y(4) and P2X(3) receptors. This study indicates that P2X and P2Y receptor subtypes are expressed by cholinergic parasympathetic neurons innervating the urinary bladder. The neurons were also stained for NF200, usually regarded as a marker for large sensory neurons. These novel histochemical properties of cholinergic neurons in the cat bladder suggest that the parasympathetic pathways to the cat bladder may be modulated by complex purinergic synaptic mechanisms.

Authors+Show Affiliations

Autonomic Neuroscience Centre, Royal Free and Univ. College Medical School, Rowland Hill St., London NW3 2PF, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16332929

Citation

Ruan, Huai Zhen, et al. "Expression of P2X and P2Y Receptors in the Intramural Parasympathetic Ganglia of the Cat Urinary Bladder." American Journal of Physiology. Renal Physiology, vol. 290, no. 5, 2006, pp. F1143-52.
Ruan HZ, Birder LA, Xiang Z, et al. Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder. Am J Physiol Renal Physiol. 2006;290(5):F1143-52.
Ruan, H. Z., Birder, L. A., Xiang, Z., Chopra, B., Buffington, T., Tai, C., ... Burnstock, G. (2006). Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder. American Journal of Physiology. Renal Physiology, 290(5), pp. F1143-52.
Ruan HZ, et al. Expression of P2X and P2Y Receptors in the Intramural Parasympathetic Ganglia of the Cat Urinary Bladder. Am J Physiol Renal Physiol. 2006;290(5):F1143-52. PubMed PMID: 16332929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder. AU - Ruan,Huai Zhen, AU - Birder,Lori A, AU - Xiang,Zhenghua, AU - Chopra,Bikramjit, AU - Buffington,Tony, AU - Tai,Changfeng, AU - Roppolo,James R, AU - de Groat,William C, AU - Burnstock,Geoffrey, Y1 - 2005/12/06/ PY - 2005/12/8/pubmed PY - 2006/5/23/medline PY - 2005/12/8/entrez SP - F1143 EP - 52 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 290 IS - 5 N2 - The distribution and function of P2X and P2Y receptor subtypes were investigated on intact or cultured intramural ganglia of the cat urinary bladder by immunocytochemistry and calcium-imaging techniques, respectively. Neurons were labeled by all seven P2X receptor subtype antibodies and antibodies for P2Y(2), P2Y(4), P2Y(6), and P2Y(12) receptor subtypes with a staining intensity of immunoreactivity in the following order: P2X(3)=P2Y(2)=P2Y(4)=P2Y(6)=P2Y(12)>P2X(1)=P2X(2)=P2X(4)>P2X(5)=P2X(6)=P2X(7). P2Y(1) receptor antibodies labeled glial cells, but not neurons. P2X(3) and P2Y(4) polyclonal antibodies labeled approximately 95 and 40% of neurons, respectively. Double staining showed that 100, 48.8, and 97.4% of P2X(3) receptor-positive neurons coexpressed choline acetyl transferase (ChAT), nitric oxide synthase (NOS), and neurofilament 200 (NF200), respectively, whereas 100, 59.2, and 97.6% of P2Y(4) receptor-positive neurons coexpressed ChAT, NOS, and NF200, respectively. Application of ATP, alpha,beta-methylene ATP, and uridine triphosphate elevated intracellular Ca(2+) concentration in a subpopulation of dissociated cultured cat intramural ganglia neurons, demonstrating the presence of functional P2Y(4) and P2X(3) receptors. This study indicates that P2X and P2Y receptor subtypes are expressed by cholinergic parasympathetic neurons innervating the urinary bladder. The neurons were also stained for NF200, usually regarded as a marker for large sensory neurons. These novel histochemical properties of cholinergic neurons in the cat bladder suggest that the parasympathetic pathways to the cat bladder may be modulated by complex purinergic synaptic mechanisms. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/16332929/Expression_of_P2X_and_P2Y_receptors_in_the_intramural_parasympathetic_ganglia_of_the_cat_urinary_bladder_ L2 - http://www.physiology.org/doi/full/10.1152/ajprenal.00333.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -