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IL-10 can induce the expression of EBV-encoded latent membrane protein-1 (LMP-1) in the absence of EBNA-2 in B lymphocytes and in Burkitt lymphoma- and NK lymphoma-derived cell lines.
Blood. 2006 Apr 01; 107(7):2928-35.Blood

Abstract

EBV-positive nasopharyngeal carcinoma and Hodgkin, T, and natural killer (NK) lymphomas express EBNA-1 and the latent membrane proteins (LMP1-2; type II latency). In contrast to type III EBV-transformed lymphoblastoid cell lines, in these cells the LMPs are expressed in the absence of EBNA-2. We have previously reported that exposure to CD40 ligand and IL-4 could induce LMP-1 in an in vitro EBV-infected Hodgkin lymphoma-derived cell line, which expressed only EBNA-1. We show now that both human and EBV-encoded IL-10 can induce LMP-1 in the absence of EBNA-2 in the Daudi, P3HR1, and other BL cell lines. Interestingly, induction of LMP-1 was not accompanied by the downregulation of BCL-6. IL-10 could also induce LMP-1 in the conditional lymphoblastoid cell line ER/EB2-5 where EBNA-2 was downregulated in the absence of estrogen. Moreover, IL-10 could induce the expression of LMP-1 in tonsillar B cells infected with the nontransforming, EBNA-2-deficient EBV strain P3HR1 and enhance LMP-1 expression in 2 EBV-positive NK lymphoma lines. The demonstration that IL-10 can induce the expression of LMP-1 in an EBNA-2-independent manner shows that the major transforming EBV gene LMP-1 can be induced by extracellular signals in lymphoid cells, and IL-10 might contribute to the establishment of type II EBV latency.

Authors+Show Affiliations

Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden. kis.lorand.levente@mtc.ki.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16332968

Citation

Kis, Lorand L., et al. "IL-10 Can Induce the Expression of EBV-encoded Latent Membrane Protein-1 (LMP-1) in the Absence of EBNA-2 in B Lymphocytes and in Burkitt Lymphoma- and NK Lymphoma-derived Cell Lines." Blood, vol. 107, no. 7, 2006, pp. 2928-35.
Kis LL, Takahara M, Nagy N, et al. IL-10 can induce the expression of EBV-encoded latent membrane protein-1 (LMP-1) in the absence of EBNA-2 in B lymphocytes and in Burkitt lymphoma- and NK lymphoma-derived cell lines. Blood. 2006;107(7):2928-35.
Kis, L. L., Takahara, M., Nagy, N., Klein, G., & Klein, E. (2006). IL-10 can induce the expression of EBV-encoded latent membrane protein-1 (LMP-1) in the absence of EBNA-2 in B lymphocytes and in Burkitt lymphoma- and NK lymphoma-derived cell lines. Blood, 107(7), 2928-35.
Kis LL, et al. IL-10 Can Induce the Expression of EBV-encoded Latent Membrane Protein-1 (LMP-1) in the Absence of EBNA-2 in B Lymphocytes and in Burkitt Lymphoma- and NK Lymphoma-derived Cell Lines. Blood. 2006 Apr 1;107(7):2928-35. PubMed PMID: 16332968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-10 can induce the expression of EBV-encoded latent membrane protein-1 (LMP-1) in the absence of EBNA-2 in B lymphocytes and in Burkitt lymphoma- and NK lymphoma-derived cell lines. AU - Kis,Lorand L, AU - Takahara,Miki, AU - Nagy,Noemi, AU - Klein,George, AU - Klein,Eva, Y1 - 2005/12/06/ PY - 2005/12/8/pubmed PY - 2006/5/9/medline PY - 2005/12/8/entrez SP - 2928 EP - 35 JF - Blood JO - Blood VL - 107 IS - 7 N2 - EBV-positive nasopharyngeal carcinoma and Hodgkin, T, and natural killer (NK) lymphomas express EBNA-1 and the latent membrane proteins (LMP1-2; type II latency). In contrast to type III EBV-transformed lymphoblastoid cell lines, in these cells the LMPs are expressed in the absence of EBNA-2. We have previously reported that exposure to CD40 ligand and IL-4 could induce LMP-1 in an in vitro EBV-infected Hodgkin lymphoma-derived cell line, which expressed only EBNA-1. We show now that both human and EBV-encoded IL-10 can induce LMP-1 in the absence of EBNA-2 in the Daudi, P3HR1, and other BL cell lines. Interestingly, induction of LMP-1 was not accompanied by the downregulation of BCL-6. IL-10 could also induce LMP-1 in the conditional lymphoblastoid cell line ER/EB2-5 where EBNA-2 was downregulated in the absence of estrogen. Moreover, IL-10 could induce the expression of LMP-1 in tonsillar B cells infected with the nontransforming, EBNA-2-deficient EBV strain P3HR1 and enhance LMP-1 expression in 2 EBV-positive NK lymphoma lines. The demonstration that IL-10 can induce the expression of LMP-1 in an EBNA-2-independent manner shows that the major transforming EBV gene LMP-1 can be induced by extracellular signals in lymphoid cells, and IL-10 might contribute to the establishment of type II EBV latency. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/16332968/IL_10_can_induce_the_expression_of_EBV_encoded_latent_membrane_protein_1__LMP_1__in_the_absence_of_EBNA_2_in_B_lymphocytes_and_in_Burkitt_lymphoma__and_NK_lymphoma_derived_cell_lines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-4971(20)65636-1 DB - PRIME DP - Unbound Medicine ER -