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Bacterial diversity in water samples from uranium wastes as demonstrated by 16S rDNA and ribosomal intergenic spacer amplification retrievals.
Can J Microbiol. 2005 Nov; 51(11):910-23.CJ

Abstract

Bacterial diversity was assessed in water samples collected from several uranium mining wastes in Ger many and in the United States by using 16S rDNA and ribosomal intergenic spacer amplification retrievals. The results obtained using the 16S rDNA retrieval showed that the samples collected from the uranium mill tailings of Schlema/Alberoda, Germany, were predominated by Nitrospina-like bacteria, whereas those from the mill tailings of Shiprock, New Mexico, USA, were predominated by gamma-Pseudomonas and Frauteria spp. Additional smaller populations of the Cytophaga-Flavobacterium-Bacteroides group and alpha- and delta-Proteobacteria were identified in the Shiprock samples as well. Proteobacteria and Cytophaga-Flavobacterium-Bacteroides were also found in the third uranium mill tailings studied, Gittersee/Coschütz, Germany, but the groups of the predominant clones were rather small. Most of the clones of the Gittersee/Coschütz samples represented individual sequences, which indicates a high level of bacterial diversity. The samples from the fourth uranium waste studied, Steinsee Deponie B1, Germany, were predominantly occupied by Acinetobacter spp. The ribosomal intergenic spacer amplification retrieval provided results complementary to those obtained by the 16S rDNA analyses. For instance, in the Shiprock samples, an additional predominant bacterial group was identified and affiliated with Nitrosomonas sp., whereas in the Gittersee/Coschütz samples, anammox populations were identified that were not retrieved by the applied 16S rDNA approach.

Authors+Show Affiliations

Institute of Radiochemistry, Forschungszentrum Rossendorf, D-10314 Dresden, Germany. gradeva@obzor.bio21.bas.bgNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16333330

Citation

Radeva, Galina, and Sonja Selenska-Pobell. "Bacterial Diversity in Water Samples From Uranium Wastes as Demonstrated By 16S rDNA and Ribosomal Intergenic Spacer Amplification Retrievals." Canadian Journal of Microbiology, vol. 51, no. 11, 2005, pp. 910-23.
Radeva G, Selenska-Pobell S. Bacterial diversity in water samples from uranium wastes as demonstrated by 16S rDNA and ribosomal intergenic spacer amplification retrievals. Can J Microbiol. 2005;51(11):910-23.
Radeva, G., & Selenska-Pobell, S. (2005). Bacterial diversity in water samples from uranium wastes as demonstrated by 16S rDNA and ribosomal intergenic spacer amplification retrievals. Canadian Journal of Microbiology, 51(11), 910-23.
Radeva G, Selenska-Pobell S. Bacterial Diversity in Water Samples From Uranium Wastes as Demonstrated By 16S rDNA and Ribosomal Intergenic Spacer Amplification Retrievals. Can J Microbiol. 2005;51(11):910-23. PubMed PMID: 16333330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacterial diversity in water samples from uranium wastes as demonstrated by 16S rDNA and ribosomal intergenic spacer amplification retrievals. AU - Radeva,Galina, AU - Selenska-Pobell,Sonja, PY - 2005/12/8/pubmed PY - 2006/6/16/medline PY - 2005/12/8/entrez SP - 910 EP - 23 JF - Canadian journal of microbiology JO - Can J Microbiol VL - 51 IS - 11 N2 - Bacterial diversity was assessed in water samples collected from several uranium mining wastes in Ger many and in the United States by using 16S rDNA and ribosomal intergenic spacer amplification retrievals. The results obtained using the 16S rDNA retrieval showed that the samples collected from the uranium mill tailings of Schlema/Alberoda, Germany, were predominated by Nitrospina-like bacteria, whereas those from the mill tailings of Shiprock, New Mexico, USA, were predominated by gamma-Pseudomonas and Frauteria spp. Additional smaller populations of the Cytophaga-Flavobacterium-Bacteroides group and alpha- and delta-Proteobacteria were identified in the Shiprock samples as well. Proteobacteria and Cytophaga-Flavobacterium-Bacteroides were also found in the third uranium mill tailings studied, Gittersee/Coschütz, Germany, but the groups of the predominant clones were rather small. Most of the clones of the Gittersee/Coschütz samples represented individual sequences, which indicates a high level of bacterial diversity. The samples from the fourth uranium waste studied, Steinsee Deponie B1, Germany, were predominantly occupied by Acinetobacter spp. The ribosomal intergenic spacer amplification retrieval provided results complementary to those obtained by the 16S rDNA analyses. For instance, in the Shiprock samples, an additional predominant bacterial group was identified and affiliated with Nitrosomonas sp., whereas in the Gittersee/Coschütz samples, anammox populations were identified that were not retrieved by the applied 16S rDNA approach. SN - 0008-4166 UR - https://www.unboundmedicine.com/medline/citation/16333330/Bacterial_diversity_in_water_samples_from_uranium_wastes_as_demonstrated_by_16S_rDNA_and_ribosomal_intergenic_spacer_amplification_retrievals_ L2 - https://cdnsciencepub.com/doi/10.1139/w05-082?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -