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Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L.
Theor Appl Genet. 2006 Feb; 112(3):509-16.TA

Abstract

Sclerotinia stem rot, caused by fungus Sclerotinia sclerotiorum, is one of the most devastating diseases in rapeseed (Brassica napus L.). We report the identification of Quantitative trait loci (QTL) involved in the resistance to S. sclerotiorum in two segregating populations of DH lines: the HUA population, derived from a cross between a partially resistant Chinese winter line (Hua dbl2) and a susceptible European spring line (P1804); and the MS population, derived from a partially resistant French winter cultivar (Major) and a susceptible Canadian spring cultivar (Stellar). A petiole inoculation technique and two scoring methods, days to wilt (DW) and stem lesion length (SLL), were used for the resistance assessment. A total of eight genomic regions affecting resistance were detected in the HUA population, with four of these regions affecting both measures of resistance. Only one region, which affected both measurements, was detected in the MS population. Individual QTL explained 6-22% of the variance. At five of the QTL from both populations, alleles from the resistant parent contributed to the resistance. QTL on N2 from the HUA population had the highest LOD score and R (2) value and was detected for SLL in the first evaluation. The N12 resistance allele in Hua dbl2 was detected in a region containing a homeologous non-reciprocal transposition (HNRT) from the resistance-containing portion of N2. This result suggests that QTL in the N12.N2 HNRT enhanced the resistance of Hua dbl2 by increasing the dosage of resistance genes. The relationship of QTL from different genetic backgrounds and their associations with other agronomic traits are discussed.

Authors+Show Affiliations

Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA. jianweizhao@mail.hzau.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16333614

Citation

Zhao, Jianwei, et al. "Quantitative Trait Loci for Resistance to Sclerotinia Sclerotiorum and Its Association With a Homeologous Non-reciprocal Transposition in Brassica Napus L." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 112, no. 3, 2006, pp. 509-16.
Zhao J, Udall JA, Quijada PA, et al. Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. Theor Appl Genet. 2006;112(3):509-16.
Zhao, J., Udall, J. A., Quijada, P. A., Grau, C. R., Meng, J., & Osborn, T. C. (2006). Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 112(3), 509-16.
Zhao J, et al. Quantitative Trait Loci for Resistance to Sclerotinia Sclerotiorum and Its Association With a Homeologous Non-reciprocal Transposition in Brassica Napus L. Theor Appl Genet. 2006;112(3):509-16. PubMed PMID: 16333614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. AU - Zhao,Jianwei, AU - Udall,Joshua A, AU - Quijada,Pablo A, AU - Grau,Craig R, AU - Meng,Jinling, AU - Osborn,Thomas C, Y1 - 2005/12/07/ PY - 2005/06/23/received PY - 2005/11/13/accepted PY - 2005/12/8/pubmed PY - 2006/4/7/medline PY - 2005/12/8/entrez SP - 509 EP - 16 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 112 IS - 3 N2 - Sclerotinia stem rot, caused by fungus Sclerotinia sclerotiorum, is one of the most devastating diseases in rapeseed (Brassica napus L.). We report the identification of Quantitative trait loci (QTL) involved in the resistance to S. sclerotiorum in two segregating populations of DH lines: the HUA population, derived from a cross between a partially resistant Chinese winter line (Hua dbl2) and a susceptible European spring line (P1804); and the MS population, derived from a partially resistant French winter cultivar (Major) and a susceptible Canadian spring cultivar (Stellar). A petiole inoculation technique and two scoring methods, days to wilt (DW) and stem lesion length (SLL), were used for the resistance assessment. A total of eight genomic regions affecting resistance were detected in the HUA population, with four of these regions affecting both measures of resistance. Only one region, which affected both measurements, was detected in the MS population. Individual QTL explained 6-22% of the variance. At five of the QTL from both populations, alleles from the resistant parent contributed to the resistance. QTL on N2 from the HUA population had the highest LOD score and R (2) value and was detected for SLL in the first evaluation. The N12 resistance allele in Hua dbl2 was detected in a region containing a homeologous non-reciprocal transposition (HNRT) from the resistance-containing portion of N2. This result suggests that QTL in the N12.N2 HNRT enhanced the resistance of Hua dbl2 by increasing the dosage of resistance genes. The relationship of QTL from different genetic backgrounds and their associations with other agronomic traits are discussed. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16333614/Quantitative_trait_loci_for_resistance_to_Sclerotinia_sclerotiorum_and_its_association_with_a_homeologous_non_reciprocal_transposition_in_Brassica_napus_L_ L2 - https://dx.doi.org/10.1007/s00122-005-0154-5 DB - PRIME DP - Unbound Medicine ER -