Tags

Type your tag names separated by a space and hit enter

CpG island methylation as an early event during adenoma progression in carcinogenesis of sporadic colorectal cancer.
J Gastroenterol Hepatol. 2005 Dec; 20(12):1920-6.JG

Abstract

BACKGROUND AND AIMS

CpG island methylation is present in various tumors, including colorectal carcinomas, and is thought to be an important mechanism in carcinogenesis. We evaluated the methylation status of primary colorectal tumors to determine its role in the adenoma to carcinoma sequence.

METHODS

The methylation status of APC, THBS1, MGMT, hMLH1 and GSTP1, as determined by methylation specific PCR (MSP), and microsatellite instability (MSI) using three mononucleotide markers were assessed in 40 colorectal adenomas and 36 adenocarcinomas. The correlations of methylation status and MSI with the clinicopathologic parameters of the tumors were determined.

RESULTS

Of the 40 adenomas, 24 (60%) were methylated at one or more loci, and 12 (30%) at two or more loci (CpG island methylation phenotype-high, CIMP-H). Of 36 carcinomas, 27 (75%) were methylated at one or more loci and 11 (30.5%) at two or more loci (CIMP-H). THBSI was the most frequently methylated locus in both adenomas (n = 19, 47.5%) and carcinomas (n = 16, 44.4%). Overall, methylation status of adenomas and carcinomas did not differ significantly (P = 0.53), nor did the methylation status of individual genes. For adenomas, size (P = 0.049) and histologic classification of the villous components (P = 0.018) were each associated with methylation status. For carcinomas, however, no clinicopathologic variable was related to methylation status. MSI was detected in three adenomas (7.5%) and five carcinomas (13.9%), and was closely correlated with hMLH1 methylation (P < 0.001).

CONCLUSIONS

In colorectal tumors, CpG island methylation of tumor suppressor genes appears to be common and may be involved in the progression of adenomas.

Authors+Show Affiliations

Department of Surgery, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea. hckim@amc.seoul.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16336454

Citation

Kim, Hee Cheol, et al. "CpG Island Methylation as an Early Event During Adenoma Progression in Carcinogenesis of Sporadic Colorectal Cancer." Journal of Gastroenterology and Hepatology, vol. 20, no. 12, 2005, pp. 1920-6.
Kim HC, Roh SA, Ga IH, et al. CpG island methylation as an early event during adenoma progression in carcinogenesis of sporadic colorectal cancer. J Gastroenterol Hepatol. 2005;20(12):1920-6.
Kim, H. C., Roh, S. A., Ga, I. H., Kim, J. S., Yu, C. S., & Kim, J. C. (2005). CpG island methylation as an early event during adenoma progression in carcinogenesis of sporadic colorectal cancer. Journal of Gastroenterology and Hepatology, 20(12), 1920-6.
Kim HC, et al. CpG Island Methylation as an Early Event During Adenoma Progression in Carcinogenesis of Sporadic Colorectal Cancer. J Gastroenterol Hepatol. 2005;20(12):1920-6. PubMed PMID: 16336454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CpG island methylation as an early event during adenoma progression in carcinogenesis of sporadic colorectal cancer. AU - Kim,Hee Cheol, AU - Roh,Sun Ae, AU - Ga,In Hwa, AU - Kim,Jung-Sun, AU - Yu,Chang Sik, AU - Kim,Jin Cheon, PY - 2005/12/13/pubmed PY - 2006/3/1/medline PY - 2005/12/13/entrez SP - 1920 EP - 6 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 20 IS - 12 N2 - BACKGROUND AND AIMS: CpG island methylation is present in various tumors, including colorectal carcinomas, and is thought to be an important mechanism in carcinogenesis. We evaluated the methylation status of primary colorectal tumors to determine its role in the adenoma to carcinoma sequence. METHODS: The methylation status of APC, THBS1, MGMT, hMLH1 and GSTP1, as determined by methylation specific PCR (MSP), and microsatellite instability (MSI) using three mononucleotide markers were assessed in 40 colorectal adenomas and 36 adenocarcinomas. The correlations of methylation status and MSI with the clinicopathologic parameters of the tumors were determined. RESULTS: Of the 40 adenomas, 24 (60%) were methylated at one or more loci, and 12 (30%) at two or more loci (CpG island methylation phenotype-high, CIMP-H). Of 36 carcinomas, 27 (75%) were methylated at one or more loci and 11 (30.5%) at two or more loci (CIMP-H). THBSI was the most frequently methylated locus in both adenomas (n = 19, 47.5%) and carcinomas (n = 16, 44.4%). Overall, methylation status of adenomas and carcinomas did not differ significantly (P = 0.53), nor did the methylation status of individual genes. For adenomas, size (P = 0.049) and histologic classification of the villous components (P = 0.018) were each associated with methylation status. For carcinomas, however, no clinicopathologic variable was related to methylation status. MSI was detected in three adenomas (7.5%) and five carcinomas (13.9%), and was closely correlated with hMLH1 methylation (P < 0.001). CONCLUSIONS: In colorectal tumors, CpG island methylation of tumor suppressor genes appears to be common and may be involved in the progression of adenomas. SN - 0815-9319 UR - https://www.unboundmedicine.com/medline/citation/16336454/CpG_island_methylation_as_an_early_event_during_adenoma_progression_in_carcinogenesis_of_sporadic_colorectal_cancer_ L2 - https://doi.org/10.1111/j.1440-1746.2005.03943.x DB - PRIME DP - Unbound Medicine ER -