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Antagonistic effects of nobiletin, a polymethoxyflavonoid, on eosinophilic airway inflammation of asthmatic rats and relevant mechanisms.
Life Sci. 2006 May 01; 78(23):2689-96.LS

Abstract

Eosinophils are known to be the important effector cells in asthmatic airway inflammation. The purpose of this study was to investigate the effects of nobiletin, a polymethoxyflavonoid, on eosinophilic airway inflammation of asthmatic rats, and explore its possible mechanisms. Animals were actively sensitized by subcutaneous injection of ovalbumin (OVA). The inflammation in lung tissues of asthmatic rats was observed by hematoxylin and eosin (HE) staining. The eosinophils in blood and BALF were separated by Percoll density gradient centrifugation and counted under microscope. The level of Eotaxin was detected by enzyme-linked immunosorbent assay (ELISA). In addition, the apoptosis of eosinophils was labeled by TdT-mediated dUTP nick end labeling (TUNEL) technique, the semi-quantitative detection for Fas mRNA expression of eosinophils was performed by reverse transcription-polymerase chain reaction (RT-PCR). The airway inflammation of asthmatic rats pretreated with nobiletin was obviously alleviated. Nobiletin (1.5 and 5.0 mg/kg given intraperitoneally) significantly reduced OVA-induced increases in eosinophils, remarkably lowered the level of Eotaxin in blood and broncho-alveolar lavage fluid (BALF) of asthmatic rats. On the other hand, semi-quantitative RT-PCR analysis for Fas of eosinophils from OVA aerosol-challenged sensitized rats showed that Fas mRNA expression of eosinophils was obviously enhanced by nobiletin. Meanwhile, the apoptosis index of cultured eosinophils was significantly elevated after treatment with different doses of nobiletin. These results indicated that nobiletin could inhibit the eosinophilic airway inflammation. Lowering the levels of Eotaxin, relieving airway infiltration of eosinophils and promoting apoptosis of eosinophils by enhancing expression of Fas mRNA may be important mechanisms for nobiletin to antagonize eosinophilic airway inflammation of asthmatic rats.

Authors+Show Affiliations

Department of Pharmacology, Nanjing Medical University, Nanjing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16337971

Citation

Wu, Yu-Qing, et al. "Antagonistic Effects of Nobiletin, a Polymethoxyflavonoid, On Eosinophilic Airway Inflammation of Asthmatic Rats and Relevant Mechanisms." Life Sciences, vol. 78, no. 23, 2006, pp. 2689-96.
Wu YQ, Zhou CH, Tao J, et al. Antagonistic effects of nobiletin, a polymethoxyflavonoid, on eosinophilic airway inflammation of asthmatic rats and relevant mechanisms. Life Sci. 2006;78(23):2689-96.
Wu, Y. Q., Zhou, C. H., Tao, J., & Li, S. N. (2006). Antagonistic effects of nobiletin, a polymethoxyflavonoid, on eosinophilic airway inflammation of asthmatic rats and relevant mechanisms. Life Sciences, 78(23), 2689-96.
Wu YQ, et al. Antagonistic Effects of Nobiletin, a Polymethoxyflavonoid, On Eosinophilic Airway Inflammation of Asthmatic Rats and Relevant Mechanisms. Life Sci. 2006 May 1;78(23):2689-96. PubMed PMID: 16337971.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antagonistic effects of nobiletin, a polymethoxyflavonoid, on eosinophilic airway inflammation of asthmatic rats and relevant mechanisms. AU - Wu,Yu-Qing, AU - Zhou,Cheng-Hua, AU - Tao,Jin, AU - Li,Sheng-Nan, Y1 - 2005/12/09/ PY - 2005/07/10/received PY - 2005/10/21/accepted PY - 2005/12/13/pubmed PY - 2006/5/26/medline PY - 2005/12/13/entrez SP - 2689 EP - 96 JF - Life sciences JO - Life Sci VL - 78 IS - 23 N2 - Eosinophils are known to be the important effector cells in asthmatic airway inflammation. The purpose of this study was to investigate the effects of nobiletin, a polymethoxyflavonoid, on eosinophilic airway inflammation of asthmatic rats, and explore its possible mechanisms. Animals were actively sensitized by subcutaneous injection of ovalbumin (OVA). The inflammation in lung tissues of asthmatic rats was observed by hematoxylin and eosin (HE) staining. The eosinophils in blood and BALF were separated by Percoll density gradient centrifugation and counted under microscope. The level of Eotaxin was detected by enzyme-linked immunosorbent assay (ELISA). In addition, the apoptosis of eosinophils was labeled by TdT-mediated dUTP nick end labeling (TUNEL) technique, the semi-quantitative detection for Fas mRNA expression of eosinophils was performed by reverse transcription-polymerase chain reaction (RT-PCR). The airway inflammation of asthmatic rats pretreated with nobiletin was obviously alleviated. Nobiletin (1.5 and 5.0 mg/kg given intraperitoneally) significantly reduced OVA-induced increases in eosinophils, remarkably lowered the level of Eotaxin in blood and broncho-alveolar lavage fluid (BALF) of asthmatic rats. On the other hand, semi-quantitative RT-PCR analysis for Fas of eosinophils from OVA aerosol-challenged sensitized rats showed that Fas mRNA expression of eosinophils was obviously enhanced by nobiletin. Meanwhile, the apoptosis index of cultured eosinophils was significantly elevated after treatment with different doses of nobiletin. These results indicated that nobiletin could inhibit the eosinophilic airway inflammation. Lowering the levels of Eotaxin, relieving airway infiltration of eosinophils and promoting apoptosis of eosinophils by enhancing expression of Fas mRNA may be important mechanisms for nobiletin to antagonize eosinophilic airway inflammation of asthmatic rats. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/16337971/Antagonistic_effects_of_nobiletin_a_polymethoxyflavonoid_on_eosinophilic_airway_inflammation_of_asthmatic_rats_and_relevant_mechanisms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(05)01139-2 DB - PRIME DP - Unbound Medicine ER -