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Transcriptome analysis of Paracoccidioides brasiliensis cells undergoing mycelium-to-yeast transition.
Eukaryot Cell. 2005 Dec; 4(12):2115-28.EC

Abstract

Paracoccidioides brasiliensis is a thermodimorphic fungus associated with paracoccidioidomycosis (PCM), a systemic mycosis prevalent in South America. In humans, infection starts by inhalation of fungal propagules, which reach the pulmonary epithelium and transform into the yeast parasitic form. Thus, the mycelium-to-yeast transition is of particular interest because conversion to yeast is essential for infection. We have used a P. brasiliensis biochip carrying sequences of 4,692 genes from this fungus to monitor gene expression at several time points of the mycelium-to-yeast morphological shift (from 5 to 120 h). The results revealed a total of 2,583 genes that displayed statistically significant modulation in at least one experimental time point. Among the identified gene homologues, some encoded enzymes involved in amino acid catabolism, signal transduction, protein synthesis, cell wall metabolism, genome structure, oxidative stress response, growth control, and development. The expression pattern of 20 genes was independently verified by real-time reverse transcription-PCR, revealing a high degree of correlation between the data obtained with the two methodologies. One gene, encoding 4-hydroxyl-phenyl pyruvate dioxygenase (4-HPPD), was highly overexpressed during the mycelium-to-yeast differentiation, and the use of NTBC [2-(2-nitro-4-trifluoromethylbenzoyl)-cyclohexane-1,3-dione], a specific inhibitor of 4-HPPD activity, as well as that of NTBC derivatives, was able to inhibit growth and differentiation of the pathogenic yeast phase of the fungus in vitro. These data set the stage for further studies involving NTBC and its derivatives as new chemotherapeutic agents against PCM and confirm the potential of array-based approaches to identify new targets for the development of alternative treatments against pathogenic microorganisms.

Authors+Show Affiliations

Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16339729

Citation

Nunes, Luiz R., et al. "Transcriptome Analysis of Paracoccidioides Brasiliensis Cells Undergoing Mycelium-to-yeast Transition." Eukaryotic Cell, vol. 4, no. 12, 2005, pp. 2115-28.
Nunes LR, Costa de Oliveira R, Leite DB, et al. Transcriptome analysis of Paracoccidioides brasiliensis cells undergoing mycelium-to-yeast transition. Eukaryotic Cell. 2005;4(12):2115-28.
Nunes, L. R., Costa de Oliveira, R., Leite, D. B., da Silva, V. S., dos Reis Marques, E., da Silva Ferreira, M. E., Ribeiro, D. C., de Souza Bernardes, L. A., Goldman, M. H., Puccia, R., Travassos, L. R., Batista, W. L., Nóbrega, M. P., Nobrega, F. G., Yang, D. Y., de Bragança Pereira, C. A., & Goldman, G. H. (2005). Transcriptome analysis of Paracoccidioides brasiliensis cells undergoing mycelium-to-yeast transition. Eukaryotic Cell, 4(12), 2115-28.
Nunes LR, et al. Transcriptome Analysis of Paracoccidioides Brasiliensis Cells Undergoing Mycelium-to-yeast Transition. Eukaryotic Cell. 2005;4(12):2115-28. PubMed PMID: 16339729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis of Paracoccidioides brasiliensis cells undergoing mycelium-to-yeast transition. AU - Nunes,Luiz R, AU - Costa de Oliveira,Regina, AU - Leite,Daniela Batista, AU - da Silva,Vivian Schmidt, AU - dos Reis Marques,Everaldo, AU - da Silva Ferreira,Márcia Eliana, AU - Ribeiro,Diógenes Custódio Duarte, AU - de Souza Bernardes,Luciano Angelo, AU - Goldman,Maria Helena S, AU - Puccia,Rosana, AU - Travassos,Luiz R, AU - Batista,Wagner L, AU - Nóbrega,Marina Pasetto, AU - Nobrega,Francisco G, AU - Yang,Ding-Yah, AU - de Bragança Pereira,Carlos A, AU - Goldman,Gustavo H, PY - 2005/12/13/pubmed PY - 2006/2/24/medline PY - 2005/12/13/entrez SP - 2115 EP - 28 JF - Eukaryotic cell JO - Eukaryotic Cell VL - 4 IS - 12 N2 - Paracoccidioides brasiliensis is a thermodimorphic fungus associated with paracoccidioidomycosis (PCM), a systemic mycosis prevalent in South America. In humans, infection starts by inhalation of fungal propagules, which reach the pulmonary epithelium and transform into the yeast parasitic form. Thus, the mycelium-to-yeast transition is of particular interest because conversion to yeast is essential for infection. We have used a P. brasiliensis biochip carrying sequences of 4,692 genes from this fungus to monitor gene expression at several time points of the mycelium-to-yeast morphological shift (from 5 to 120 h). The results revealed a total of 2,583 genes that displayed statistically significant modulation in at least one experimental time point. Among the identified gene homologues, some encoded enzymes involved in amino acid catabolism, signal transduction, protein synthesis, cell wall metabolism, genome structure, oxidative stress response, growth control, and development. The expression pattern of 20 genes was independently verified by real-time reverse transcription-PCR, revealing a high degree of correlation between the data obtained with the two methodologies. One gene, encoding 4-hydroxyl-phenyl pyruvate dioxygenase (4-HPPD), was highly overexpressed during the mycelium-to-yeast differentiation, and the use of NTBC [2-(2-nitro-4-trifluoromethylbenzoyl)-cyclohexane-1,3-dione], a specific inhibitor of 4-HPPD activity, as well as that of NTBC derivatives, was able to inhibit growth and differentiation of the pathogenic yeast phase of the fungus in vitro. These data set the stage for further studies involving NTBC and its derivatives as new chemotherapeutic agents against PCM and confirm the potential of array-based approaches to identify new targets for the development of alternative treatments against pathogenic microorganisms. SN - 1535-9778 UR - https://www.unboundmedicine.com/medline/citation/16339729/Transcriptome_analysis_of_Paracoccidioides_brasiliensis_cells_undergoing_mycelium_to_yeast_transition_ L2 - http://ec.asm.org/cgi/pmidlookup?view=long&pmid=16339729 DB - PRIME DP - Unbound Medicine ER -