Tags

Type your tag names separated by a space and hit enter

A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio.

Abstract

Delta aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on %Pb-P(plasma lead)/Pb-B(whole blood) ratio in 142 subjects environmentally exposed to lead. Genotypes for the ALAD G177C polymorphism were determined by PCR and restriction fragment length digestion. Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. The allele frequencies for ALAD1 and ALAD2 alleles were 0.897 and 0.103, respectively. We combined both ALAD 1-2 and ALAD 2-2 genotypes together (ALAD 1-2/2-2 group) and compared with the ALAD 1-1 genotype group. While no significant differences were found in Pb-B, subjects from the ALAD 1-2/2-2 genotype group presented significantly higher Pb-P concentrations and %Pb-P/Pb-B ratios (0.89+/-0.07 microg/l, and 1.45+/-0.10%, respectively) when compared with subjects from the ALAD 1-1 genotype group (0.44+/-0.05 microg/l, and 0.48+/-0.02, respectively; both P<0.0001). The higher %Pb-P/Pb-B ratios in carriers of the ALAD-2 allele compared with noncarriers indicate that ALAD 1-2/2-2 subjects are probably at increased health risks associated with lead exposure.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes 3900, 14049-900 Ribeirao Preto, SP, Brazil.

    , , ,

    Source

    Archives of toxicology 80:7 2006 Jul pg 394-8

    MeSH

    Adolescent
    Adult
    Brazil
    Environmental Exposure
    Female
    Genetic Predisposition to Disease
    Genotype
    Humans
    Lead
    Lead Poisoning
    Male
    Middle Aged
    Polymorphism, Restriction Fragment Length
    Porphobilinogen Synthase

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16341691

    Citation

    Montenegro, Marcelo F., et al. "A Polymorphism in the Delta-aminolevulinic Acid Dehydratase Gene Modifies Plasma/whole Blood Lead Ratio." Archives of Toxicology, vol. 80, no. 7, 2006, pp. 394-8.
    Montenegro MF, Barbosa F, Sandrim VC, et al. A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio. Arch Toxicol. 2006;80(7):394-8.
    Montenegro, M. F., Barbosa, F., Sandrim, V. C., Gerlach, R. F., & Tanus-Santos, J. E. (2006). A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio. Archives of Toxicology, 80(7), pp. 394-8.
    Montenegro MF, et al. A Polymorphism in the Delta-aminolevulinic Acid Dehydratase Gene Modifies Plasma/whole Blood Lead Ratio. Arch Toxicol. 2006;80(7):394-8. PubMed PMID: 16341691.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio. AU - Montenegro,Marcelo F, AU - Barbosa,Fernando,Jr AU - Sandrim,Valeria C, AU - Gerlach,Raquel F, AU - Tanus-Santos,Jose E, Y1 - 2005/12/09/ PY - 2005/11/01/received PY - 2005/11/18/accepted PY - 2005/12/13/pubmed PY - 2006/9/1/medline PY - 2005/12/13/entrez SP - 394 EP - 8 JF - Archives of toxicology JO - Arch. Toxicol. VL - 80 IS - 7 N2 - Delta aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on %Pb-P(plasma lead)/Pb-B(whole blood) ratio in 142 subjects environmentally exposed to lead. Genotypes for the ALAD G177C polymorphism were determined by PCR and restriction fragment length digestion. Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. The allele frequencies for ALAD1 and ALAD2 alleles were 0.897 and 0.103, respectively. We combined both ALAD 1-2 and ALAD 2-2 genotypes together (ALAD 1-2/2-2 group) and compared with the ALAD 1-1 genotype group. While no significant differences were found in Pb-B, subjects from the ALAD 1-2/2-2 genotype group presented significantly higher Pb-P concentrations and %Pb-P/Pb-B ratios (0.89+/-0.07 microg/l, and 1.45+/-0.10%, respectively) when compared with subjects from the ALAD 1-1 genotype group (0.44+/-0.05 microg/l, and 0.48+/-0.02, respectively; both P<0.0001). The higher %Pb-P/Pb-B ratios in carriers of the ALAD-2 allele compared with noncarriers indicate that ALAD 1-2/2-2 subjects are probably at increased health risks associated with lead exposure. SN - 0340-5761 UR - https://www.unboundmedicine.com/medline/citation/16341691/A_polymorphism_in_the_delta_aminolevulinic_acid_dehydratase_gene_modifies_plasma/whole_blood_lead_ratio_ L2 - https://dx.doi.org/10.1007/s00204-005-0056-y DB - PRIME DP - Unbound Medicine ER -