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Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation.
J Histochem Cytochem 2006; 54(5):525-37JH

Abstract

To determine the functions of fibromodulin (Fmod), a small leucine-rich keratan sulfate proteoglycan in tooth formation, we investigated the distribution of Fmod in dental tissues by immunohistochemistry and characterized the dental phenotype of 1-day-old Fmod-deficient mice using light and transmission electron microscopy. Immunohistochemistry was also used to compare the relative protein expression of dentin sialoprotein (DSP), dentin matrix protein-1 (DMP 1), bone sialoprotein (BSP), and osteopontin (OPN) between Fmod-deficient mice and wild-type mice. In normal mice and rats, Fmod immunostaining was mostly detected in the distal cell bodies of odontoblasts and in the stratum intermedium and was weaker in odontoblast processes and predentin. The absence of Fmod impaired dentin mineralization, increased the diameter of the collagen fibrils throughout the whole predentin, and delayed enamel formation. Immunohistochemistry provides evidence for compensatory mechanisms in Fmod-deficient mice. Staining for DSP and OPN was decreased in molars, whereas DMP 1 and BSP were enhanced. In the incisors, labeling for DSP, DMP 1, and BSP was strongly increased in the pulp and odontoblasts, whereas OPN staining was decreased. Positive staining was also seen for DMP 1 and BSP in secretory ameloblasts. Together these studies indicate that Fmod restricts collagen fibrillogenesis in predentin while promoting dentin mineralization and the early stages of enamel formation.

Authors+Show Affiliations

Laboratoire Réparation et Remodelage des Tissus Oro-Faciaux, EA 4296, Groupe Matrices extracellulaires et biominéralisations, Faculté de Chirurgie Dentaire, Université Paris V, Montrouge, France. mgoldod@aol.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16344330

Citation

Goldberg, Michel, et al. "Fibromodulin-deficient Mice Display Impaired Collagen Fibrillogenesis in Predentin as Well as Altered Dentin Mineralization and Enamel Formation." The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, vol. 54, no. 5, 2006, pp. 525-37.
Goldberg M, Septier D, Oldberg A, et al. Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation. J Histochem Cytochem. 2006;54(5):525-37.
Goldberg, M., Septier, D., Oldberg, A., Young, M. F., & Ameye, L. G. (2006). Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, 54(5), pp. 525-37.
Goldberg M, et al. Fibromodulin-deficient Mice Display Impaired Collagen Fibrillogenesis in Predentin as Well as Altered Dentin Mineralization and Enamel Formation. J Histochem Cytochem. 2006;54(5):525-37. PubMed PMID: 16344330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation. AU - Goldberg,Michel, AU - Septier,Dominique, AU - Oldberg,Ake, AU - Young,Marian F, AU - Ameye,Laurent G, Y1 - 2005/12/12/ PY - 2005/12/14/pubmed PY - 2006/5/19/medline PY - 2005/12/14/entrez SP - 525 EP - 37 JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society JO - J. Histochem. Cytochem. VL - 54 IS - 5 N2 - To determine the functions of fibromodulin (Fmod), a small leucine-rich keratan sulfate proteoglycan in tooth formation, we investigated the distribution of Fmod in dental tissues by immunohistochemistry and characterized the dental phenotype of 1-day-old Fmod-deficient mice using light and transmission electron microscopy. Immunohistochemistry was also used to compare the relative protein expression of dentin sialoprotein (DSP), dentin matrix protein-1 (DMP 1), bone sialoprotein (BSP), and osteopontin (OPN) between Fmod-deficient mice and wild-type mice. In normal mice and rats, Fmod immunostaining was mostly detected in the distal cell bodies of odontoblasts and in the stratum intermedium and was weaker in odontoblast processes and predentin. The absence of Fmod impaired dentin mineralization, increased the diameter of the collagen fibrils throughout the whole predentin, and delayed enamel formation. Immunohistochemistry provides evidence for compensatory mechanisms in Fmod-deficient mice. Staining for DSP and OPN was decreased in molars, whereas DMP 1 and BSP were enhanced. In the incisors, labeling for DSP, DMP 1, and BSP was strongly increased in the pulp and odontoblasts, whereas OPN staining was decreased. Positive staining was also seen for DMP 1 and BSP in secretory ameloblasts. Together these studies indicate that Fmod restricts collagen fibrillogenesis in predentin while promoting dentin mineralization and the early stages of enamel formation. SN - 0022-1554 UR - https://www.unboundmedicine.com/medline/citation/16344330/Fibromodulin_deficient_mice_display_impaired_collagen_fibrillogenesis_in_predentin_as_well_as_altered_dentin_mineralization_and_enamel_formation_ L2 - http://journals.sagepub.com/doi/full/10.1369/jhc.5A6650.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -