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Derivation of Mutants of Erwinia carotovora subsp. betavasculorum Deficient in Export of Pectolytic Enzymes with Potential for Biological Control of Potato Soft Rot.
Appl Environ Microbiol. 1994 Jul; 60(7):2278-85.AE

Abstract

Erwinia carotovora subsp. betavasculorum Ecb168 produces an antibiotic(s) that suppresses growth of the related bacterium Erwinia carotovora subsp. carotovora in culture and in wounds of potato tubers. Strain Ecb168 also produces and secretes pectolytic enzymes and causes a vascular necrosis and root rot of sugar beet. Genes (out) involved in secretion of pectolytic enzymes by Ecb168 were localized to two HindIII fragments (8.5 and 10.5 kb) of Ecb168 genomic DNA by hybridization to the cloned out region of E. carotovora subsp. carotovora and by complementation of Out mutants of E. carotovora subsp. carotovora. Out mutants of Ecb168, which did not secrete pectate lyase into the culture medium, were obtained when deletions internal to either HindIII fragment were introduced into the genome of Ecb168 through marker exchange mutagenesis. Out mutants of Ecb168 were complemented to the Out phenotype by introduction of the corresponding cloned HindIII fragment. Out mutants of Ecb168 were less virulent than the Out parental strain on potato tubers. Strain Ecb168 and Out derivatives inhibited the growth of E. carotovora subsp. carotovora in culture, indicating that the uncharacterized antibiotic(s) responsible for antagonism was exported through an out-independent mechanism. Strain Ecb168 and Out derivatives reduced the establishment of large populations of E. carotovora subsp. carotovora in wounds of potato tubers and suppressed tuber soft rot caused by E. carotovora subsp. carotovora.

Authors+Show Affiliations

Horticultural Crops Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16349316

Citation

Costa, J M., and J E. Loper. "Derivation of Mutants of Erwinia Carotovora Subsp. Betavasculorum Deficient in Export of Pectolytic Enzymes With Potential for Biological Control of Potato Soft Rot." Applied and Environmental Microbiology, vol. 60, no. 7, 1994, pp. 2278-85.
Costa JM, Loper JE. Derivation of Mutants of Erwinia carotovora subsp. betavasculorum Deficient in Export of Pectolytic Enzymes with Potential for Biological Control of Potato Soft Rot. Appl Environ Microbiol. 1994;60(7):2278-85.
Costa, J. M., & Loper, J. E. (1994). Derivation of Mutants of Erwinia carotovora subsp. betavasculorum Deficient in Export of Pectolytic Enzymes with Potential for Biological Control of Potato Soft Rot. Applied and Environmental Microbiology, 60(7), 2278-85.
Costa JM, Loper JE. Derivation of Mutants of Erwinia Carotovora Subsp. Betavasculorum Deficient in Export of Pectolytic Enzymes With Potential for Biological Control of Potato Soft Rot. Appl Environ Microbiol. 1994;60(7):2278-85. PubMed PMID: 16349316.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Derivation of Mutants of Erwinia carotovora subsp. betavasculorum Deficient in Export of Pectolytic Enzymes with Potential for Biological Control of Potato Soft Rot. AU - Costa,J M, AU - Loper,J E, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 2278 EP - 85 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 60 IS - 7 N2 - Erwinia carotovora subsp. betavasculorum Ecb168 produces an antibiotic(s) that suppresses growth of the related bacterium Erwinia carotovora subsp. carotovora in culture and in wounds of potato tubers. Strain Ecb168 also produces and secretes pectolytic enzymes and causes a vascular necrosis and root rot of sugar beet. Genes (out) involved in secretion of pectolytic enzymes by Ecb168 were localized to two HindIII fragments (8.5 and 10.5 kb) of Ecb168 genomic DNA by hybridization to the cloned out region of E. carotovora subsp. carotovora and by complementation of Out mutants of E. carotovora subsp. carotovora. Out mutants of Ecb168, which did not secrete pectate lyase into the culture medium, were obtained when deletions internal to either HindIII fragment were introduced into the genome of Ecb168 through marker exchange mutagenesis. Out mutants of Ecb168 were complemented to the Out phenotype by introduction of the corresponding cloned HindIII fragment. Out mutants of Ecb168 were less virulent than the Out parental strain on potato tubers. Strain Ecb168 and Out derivatives inhibited the growth of E. carotovora subsp. carotovora in culture, indicating that the uncharacterized antibiotic(s) responsible for antagonism was exported through an out-independent mechanism. Strain Ecb168 and Out derivatives reduced the establishment of large populations of E. carotovora subsp. carotovora in wounds of potato tubers and suppressed tuber soft rot caused by E. carotovora subsp. carotovora. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16349316/Derivation_of_Mutants_of_Erwinia_carotovora_subsp__betavasculorum_Deficient_in_Export_of_Pectolytic_Enzymes_with_Potential_for_Biological_Control_of_Potato_Soft_Rot_ L2 - https://journals.asm.org/doi/10.1128/aem.60.7.2278-2285.1994?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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