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Effects of dexamethasone on primarily cultured newly differentiated rat adipocytes from different adipose tissue regions.
Obes Res. 1993 Mar; 1(2):99-105.OR

Abstract

The effects of dexamethasone (dex) on newly differentiated adipocytes in primary culture derived from mesenteric, retroperitoneal, epididymal, and inguinal subcutaneous adipose tissues of male rats were studied. The degree of differentiation was similar in these adipose precursor cells derived from different regions as assessed by lipoprotein lipase (LPL) activity, an early marker of adipocyte differentiation. LPL activity was increased by addition of dex, and no differences in degree of activation were observed in cells from different adipose tissue regions. Development of both basal and isoproterenol-stimulated lipolysis was also similar in adipose precursor cells from different adipose tissue regions. Dex addition enhanced the isoproterenol-stimulated lipolysis with no regional differences. Studies of binding of [3H]-dex showed no regional differences in either binding affinity or maximal binding capacity. It is concluded that dex stimulates both LPL activity and lipolytic activity in newly differentiated rat adipocytes in primary culture. This seems, however, not to vary in magnitude in cells obtained from different adipose tissue regions. This might be due to the apparent similarity of number and affinity of glucocorticoid binding sites. Regional variations in glucocorticoid regulated LPL and lipolytic activity in adipose tissue might therefore not be due to inherent differences between adipocytes.

Authors+Show Affiliations

Wallenberg Laboratory, Sahlgren's Hospital, University of Gothenburg, S-413 45 Gothenberg, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16350566

Citation

Yang, S, et al. "Effects of Dexamethasone On Primarily Cultured Newly Differentiated Rat Adipocytes From Different Adipose Tissue Regions." Obesity Research, vol. 1, no. 2, 1993, pp. 99-105.
Yang S, Xu X, Björntorp P. Effects of dexamethasone on primarily cultured newly differentiated rat adipocytes from different adipose tissue regions. Obes Res. 1993;1(2):99-105.
Yang, S., Xu, X., & Björntorp, P. (1993). Effects of dexamethasone on primarily cultured newly differentiated rat adipocytes from different adipose tissue regions. Obesity Research, 1(2), 99-105.
Yang S, Xu X, Björntorp P. Effects of Dexamethasone On Primarily Cultured Newly Differentiated Rat Adipocytes From Different Adipose Tissue Regions. Obes Res. 1993;1(2):99-105. PubMed PMID: 16350566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dexamethasone on primarily cultured newly differentiated rat adipocytes from different adipose tissue regions. AU - Yang,S, AU - Xu,X, AU - Björntorp,P, PY - 1993/3/1/pubmed PY - 2006/2/8/medline PY - 1993/3/1/entrez SP - 99 EP - 105 JF - Obesity research JO - Obes. Res. VL - 1 IS - 2 N2 - The effects of dexamethasone (dex) on newly differentiated adipocytes in primary culture derived from mesenteric, retroperitoneal, epididymal, and inguinal subcutaneous adipose tissues of male rats were studied. The degree of differentiation was similar in these adipose precursor cells derived from different regions as assessed by lipoprotein lipase (LPL) activity, an early marker of adipocyte differentiation. LPL activity was increased by addition of dex, and no differences in degree of activation were observed in cells from different adipose tissue regions. Development of both basal and isoproterenol-stimulated lipolysis was also similar in adipose precursor cells from different adipose tissue regions. Dex addition enhanced the isoproterenol-stimulated lipolysis with no regional differences. Studies of binding of [3H]-dex showed no regional differences in either binding affinity or maximal binding capacity. It is concluded that dex stimulates both LPL activity and lipolytic activity in newly differentiated rat adipocytes in primary culture. This seems, however, not to vary in magnitude in cells obtained from different adipose tissue regions. This might be due to the apparent similarity of number and affinity of glucocorticoid binding sites. Regional variations in glucocorticoid regulated LPL and lipolytic activity in adipose tissue might therefore not be due to inherent differences between adipocytes. SN - 1071-7323 UR - https://www.unboundmedicine.com/medline/citation/16350566/Effects_of_dexamethasone_on_primarily_cultured_newly_differentiated_rat_adipocytes_from_different_adipose_tissue_regions_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1071-7323&date=1993&volume=1&issue=2&spage=99 DB - PRIME DP - Unbound Medicine ER -