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Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis.
J Cell Sci. 2006 Jan 01; 119(Pt 1):104-14.JC

Abstract

During determination of the cell division plane, an actomyosin contractile ring is induced at the equatorial cell cortex by signals from the mitotic apparatus and contracts to cause cleavage furrow progression. Although the small GTPase RhoA is known to regulate the progression, probably by controlling actin filament assembly and enhancing actomyosin interaction, any involvement of RhoA in division plane determination is unknown. In this study, using a trichloroacetic acid (TCA) fixation protocol we recently developed, we show that RhoA accumulates at the equatorial cortex before furrow initiation and continues to concentrate at the cleavage furrow during cytokinesis. We also demonstrate that both Rho activity and microtubule organization are required for RhoA localization and proper furrowing. Selective disruption of microtubule organization revealed that both astral and central spindle microtubules can recruit RhoA at the equatorial cortex. We find that centralspindlin and ECT2 are required for RhoA localization and furrowing. Centralspindlin is localized both to central spindle microtubules and at the tips of astral microtubules near the equatorial cortex and recruits ECT2. Positional information for division plane determination from microtubules is transmitted to the cell cortex to organize actin cytoskeleton through a mechanism involving these proteins.

Authors+Show Affiliations

Laboratory for Cellular Morphogenesis, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16352658

Citation

Nishimura, Yukako, and Shigenobu Yonemura. "Centralspindlin Regulates ECT2 and RhoA Accumulation at the Equatorial Cortex During Cytokinesis." Journal of Cell Science, vol. 119, no. Pt 1, 2006, pp. 104-14.
Nishimura Y, Yonemura S. Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis. J Cell Sci. 2006;119(Pt 1):104-14.
Nishimura, Y., & Yonemura, S. (2006). Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis. Journal of Cell Science, 119(Pt 1), 104-14.
Nishimura Y, Yonemura S. Centralspindlin Regulates ECT2 and RhoA Accumulation at the Equatorial Cortex During Cytokinesis. J Cell Sci. 2006 Jan 1;119(Pt 1):104-14. PubMed PMID: 16352658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis. AU - Nishimura,Yukako, AU - Yonemura,Shigenobu, Y1 - 2005/12/13/ PY - 2005/12/15/pubmed PY - 2006/12/16/medline PY - 2005/12/15/entrez SP - 104 EP - 14 JF - Journal of cell science JO - J. Cell. Sci. VL - 119 IS - Pt 1 N2 - During determination of the cell division plane, an actomyosin contractile ring is induced at the equatorial cell cortex by signals from the mitotic apparatus and contracts to cause cleavage furrow progression. Although the small GTPase RhoA is known to regulate the progression, probably by controlling actin filament assembly and enhancing actomyosin interaction, any involvement of RhoA in division plane determination is unknown. In this study, using a trichloroacetic acid (TCA) fixation protocol we recently developed, we show that RhoA accumulates at the equatorial cortex before furrow initiation and continues to concentrate at the cleavage furrow during cytokinesis. We also demonstrate that both Rho activity and microtubule organization are required for RhoA localization and proper furrowing. Selective disruption of microtubule organization revealed that both astral and central spindle microtubules can recruit RhoA at the equatorial cortex. We find that centralspindlin and ECT2 are required for RhoA localization and furrowing. Centralspindlin is localized both to central spindle microtubules and at the tips of astral microtubules near the equatorial cortex and recruits ECT2. Positional information for division plane determination from microtubules is transmitted to the cell cortex to organize actin cytoskeleton through a mechanism involving these proteins. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/16352658/Centralspindlin_regulates_ECT2_and_RhoA_accumulation_at_the_equatorial_cortex_during_cytokinesis_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=16352658 DB - PRIME DP - Unbound Medicine ER -