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Hfq is a regulator of F-plasmid TraJ and TraM synthesis in Escherichia coli.
J Bacteriol. 2006 Jan; 188(1):124-31.JB

Abstract

The F plasmid of Escherichia coli allows horizontal DNA transfer between an F(+) donor cell and an F(-) recipient. Expression of the transfer genes is tightly controlled by a number of factors, including the following plasmid-encoded regulatory proteins: TraJ, the primary activator of the 33-kb tra operon, and the autoregulators TraM and TraY. Here, we demonstrate that the host RNA binding protein, Hfq, represses TraJ and TraM synthesis by destabilizing their respective mRNAs. Mating assays and immunoblot analyses for TraM and TraJ showed that transfer efficiency and protein levels increased in host cells containing a disruption in hfq compared to wild-type cells in stationary phase. The stability of transcripts containing a putative Hfq binding site located in the intergenic untranslated region between traM and traJ was increased in hfq mutant donor cells, suggesting that Hfq destabilizes these transcripts. Electrophoretic mobility shift assays demonstrated that Hfq specifically binds this region but not the antisense RNA, FinP, encoded on the opposite strand. Together, these findings indicate that Hfq regulates traM and traJ transcript stability by a mechanism separate from FinOP-mediated repression.

Authors+Show Affiliations

CW405 Biological Sciences Building, Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16352828

Citation

Will, William R., and Laura S. Frost. "Hfq Is a Regulator of F-plasmid TraJ and TraM Synthesis in Escherichia Coli." Journal of Bacteriology, vol. 188, no. 1, 2006, pp. 124-31.
Will WR, Frost LS. Hfq is a regulator of F-plasmid TraJ and TraM synthesis in Escherichia coli. J Bacteriol. 2006;188(1):124-31.
Will, W. R., & Frost, L. S. (2006). Hfq is a regulator of F-plasmid TraJ and TraM synthesis in Escherichia coli. Journal of Bacteriology, 188(1), 124-31.
Will WR, Frost LS. Hfq Is a Regulator of F-plasmid TraJ and TraM Synthesis in Escherichia Coli. J Bacteriol. 2006;188(1):124-31. PubMed PMID: 16352828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hfq is a regulator of F-plasmid TraJ and TraM synthesis in Escherichia coli. AU - Will,William R, AU - Frost,Laura S, PY - 2005/12/15/pubmed PY - 2006/3/15/medline PY - 2005/12/15/entrez SP - 124 EP - 31 JF - Journal of bacteriology JO - J Bacteriol VL - 188 IS - 1 N2 - The F plasmid of Escherichia coli allows horizontal DNA transfer between an F(+) donor cell and an F(-) recipient. Expression of the transfer genes is tightly controlled by a number of factors, including the following plasmid-encoded regulatory proteins: TraJ, the primary activator of the 33-kb tra operon, and the autoregulators TraM and TraY. Here, we demonstrate that the host RNA binding protein, Hfq, represses TraJ and TraM synthesis by destabilizing their respective mRNAs. Mating assays and immunoblot analyses for TraM and TraJ showed that transfer efficiency and protein levels increased in host cells containing a disruption in hfq compared to wild-type cells in stationary phase. The stability of transcripts containing a putative Hfq binding site located in the intergenic untranslated region between traM and traJ was increased in hfq mutant donor cells, suggesting that Hfq destabilizes these transcripts. Electrophoretic mobility shift assays demonstrated that Hfq specifically binds this region but not the antisense RNA, FinP, encoded on the opposite strand. Together, these findings indicate that Hfq regulates traM and traJ transcript stability by a mechanism separate from FinOP-mediated repression. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/16352828/Hfq_is_a_regulator_of_F_plasmid_TraJ_and_TraM_synthesis_in_Escherichia_coli_ L2 - https://journals.asm.org/doi/10.1128/JB.188.1.124-131.2006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -