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PecS and PecT coregulate the synthesis of HrpN and pectate lyases, two virulence determinants in Erwinia chrysanthemi 3937.
Mol Plant Microbe Interact. 2005 Nov; 18(11):1205-14.MP

Abstract

Erwinia chrysanthemi strain 3937 is a necrotrophic bacterial plant pathogen. Pectinolytic enzymes and, in particular, pectate lyases play a key role in soft rot symptoms; however, the efficient colonization of plants by E. chrysanthemi requires additional factors. These factors include HrpN (harpin), a heat-stable, glycine-rich hydrophilic protein, which is secreted by the type III secretion system. We investigated the expression of hrpN in E. chrysanthemi 3937 in various environmental conditions and different regulatory backgrounds. Using lacZ fusions, hrpN expression was markedly influenced by the carbon source, osmolarity, growth phase, and growth substrate. hrpN was repressed when pectinolysis started and negatively regulated by the repressors of pectate lyase synthesis, PecS and PecT. Primer extension data and in vitro DNA-protein interaction experiments support a model whereby PecS represses hrpN expression by binding to the hrpN regulatory region and inhibiting transcript elongation. The results suggest coordinated regulation of HrpN and pectate lyases by PecS and PecT. A putative model of the synthesis of these two virulence factors in E. chrysanthemi during pathogenesis is presented.

Authors+Show Affiliations

Nasser W
Unité de Microbiologie et Génétique UMR CNRS-INSA-UCBL 5122 Domaine Scientifique de la Doua, Université Claude Bernard Lyon I Bâtiment A Lwoff, Villeurbanne, France. William.nasser@insa-lyon.fr
Reverchon S
No affiliation info available
Vedel R
No affiliation info available
Boccara M
No affiliation info available

MeSH

Bacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceChicoryCulture MediaDNA, BacterialDickeya chrysanthemiGene Expression Regulation, BacterialGene Expression Regulation, EnzymologicMolecular Sequence DataOsmolar ConcentrationPlant DiseasesPolysaccharide-LyasesRepressor ProteinsTranscription FactorsVirulence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16353555

Citation

Nasser, William, et al. "PecS and PecT Coregulate the Synthesis of HrpN and Pectate Lyases, Two Virulence Determinants in Erwinia Chrysanthemi 3937." Molecular Plant-microbe Interactions : MPMI, vol. 18, no. 11, 2005, pp. 1205-14.
Nasser W, Reverchon S, Vedel R, et al. PecS and PecT coregulate the synthesis of HrpN and pectate lyases, two virulence determinants in Erwinia chrysanthemi 3937. Mol Plant Microbe Interact. 2005;18(11):1205-14.
Nasser, W., Reverchon, S., Vedel, R., & Boccara, M. (2005). PecS and PecT coregulate the synthesis of HrpN and pectate lyases, two virulence determinants in Erwinia chrysanthemi 3937. Molecular Plant-microbe Interactions : MPMI, 18(11), 1205-14.
Nasser W, et al. PecS and PecT Coregulate the Synthesis of HrpN and Pectate Lyases, Two Virulence Determinants in Erwinia Chrysanthemi 3937. Mol Plant Microbe Interact. 2005;18(11):1205-14. PubMed PMID: 16353555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PecS and PecT coregulate the synthesis of HrpN and pectate lyases, two virulence determinants in Erwinia chrysanthemi 3937. AU - Nasser,William, AU - Reverchon,Sylvie, AU - Vedel,Regine, AU - Boccara,Martine, PY - 2005/12/16/pubmed PY - 2006/3/10/medline PY - 2005/12/16/entrez SP - 1205 EP - 14 JF - Molecular plant-microbe interactions : MPMI JO - Mol Plant Microbe Interact VL - 18 IS - 11 N2 - Erwinia chrysanthemi strain 3937 is a necrotrophic bacterial plant pathogen. Pectinolytic enzymes and, in particular, pectate lyases play a key role in soft rot symptoms; however, the efficient colonization of plants by E. chrysanthemi requires additional factors. These factors include HrpN (harpin), a heat-stable, glycine-rich hydrophilic protein, which is secreted by the type III secretion system. We investigated the expression of hrpN in E. chrysanthemi 3937 in various environmental conditions and different regulatory backgrounds. Using lacZ fusions, hrpN expression was markedly influenced by the carbon source, osmolarity, growth phase, and growth substrate. hrpN was repressed when pectinolysis started and negatively regulated by the repressors of pectate lyase synthesis, PecS and PecT. Primer extension data and in vitro DNA-protein interaction experiments support a model whereby PecS represses hrpN expression by binding to the hrpN regulatory region and inhibiting transcript elongation. The results suggest coordinated regulation of HrpN and pectate lyases by PecS and PecT. A putative model of the synthesis of these two virulence factors in E. chrysanthemi during pathogenesis is presented. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/16353555/PecS_and_PecT_coregulate_the_synthesis_of_HrpN_and_pectate_lyases_two_virulence_determinants_in_Erwinia_chrysanthemi_3937_ DB - PRIME DP - Unbound Medicine ER -