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Histamine excites neonatal rat sympathetic preganglionic neurons in vitro via activation of H1 receptors.
J Neurophysiol. 2006 Apr; 95(4):2492-500.JN

Abstract

The role of histamine in regulating excitability of sympathetic preganglionic neurons (SPNs) and the expression of histamine receptor mRNA in SPNs was investigated using whole-cell patch-clamp electrophysiological recording techniques combined with single-cell reverse transcriptase polymerase chain reaction (RT-PCR) in transverse neonatal rat spinal cord slices. Bath application of histamine (100 microM) or the H1 receptor agonist histamine trifluoromethyl toluidide dimaleate (HTMT; 10 microM) induced membrane depolarization associated with a decrease in membrane conductance in the majority (70%) of SPNs tested, via activation of postsynaptic H1 receptors negatively coupled to one or more unidentified K+ conductances. Histamine and HTMT application also induced or increased the amplitude and/or frequency of membrane potential oscillations in electrotonically coupled SPNs. The H2 receptor agonist dimaprit (10 microM) or the H3 receptor agonist imetit (100 nM) were without significant effect on the membrane properties of SPNs. Histamine responses were sensitive to the H1 receptor antagonist triprolidine (10 microM) and the nonselective potassium channel blocker barium (1 mM) but were unaffected by the H2 receptor antagonist tiotidine (10 microM) and the H3 receptor antagonist, clobenpropit (5 microM). Single cell RT-PCR revealed mRNA expression for H1 receptors in 75% of SPNs tested, with no expression of mRNA for H2, H3, or H4 receptors. These data represent the first demonstration of H1 receptor expression in SPNs and suggest that histamine acts to regulate excitability of these neurons via a direct postsynaptic effect on H1 receptors.

Authors+Show Affiliations

Warwick Medical School, University of Warwick, Coventry, CV4 7AL, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16354729

Citation

Whyment, Andrew D., et al. "Histamine Excites Neonatal Rat Sympathetic Preganglionic Neurons in Vitro Via Activation of H1 Receptors." Journal of Neurophysiology, vol. 95, no. 4, 2006, pp. 2492-500.
Whyment AD, Blanks AM, Lee K, et al. Histamine excites neonatal rat sympathetic preganglionic neurons in vitro via activation of H1 receptors. J Neurophysiol. 2006;95(4):2492-500.
Whyment, A. D., Blanks, A. M., Lee, K., Renaud, L. P., & Spanswick, D. (2006). Histamine excites neonatal rat sympathetic preganglionic neurons in vitro via activation of H1 receptors. Journal of Neurophysiology, 95(4), 2492-500.
Whyment AD, et al. Histamine Excites Neonatal Rat Sympathetic Preganglionic Neurons in Vitro Via Activation of H1 Receptors. J Neurophysiol. 2006;95(4):2492-500. PubMed PMID: 16354729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Histamine excites neonatal rat sympathetic preganglionic neurons in vitro via activation of H1 receptors. AU - Whyment,Andrew D, AU - Blanks,Andrew M, AU - Lee,Kevin, AU - Renaud,Leo P, AU - Spanswick,David, Y1 - 2005/12/14/ PY - 2005/12/16/pubmed PY - 2006/9/26/medline PY - 2005/12/16/entrez SP - 2492 EP - 500 JF - Journal of neurophysiology JO - J Neurophysiol VL - 95 IS - 4 N2 - The role of histamine in regulating excitability of sympathetic preganglionic neurons (SPNs) and the expression of histamine receptor mRNA in SPNs was investigated using whole-cell patch-clamp electrophysiological recording techniques combined with single-cell reverse transcriptase polymerase chain reaction (RT-PCR) in transverse neonatal rat spinal cord slices. Bath application of histamine (100 microM) or the H1 receptor agonist histamine trifluoromethyl toluidide dimaleate (HTMT; 10 microM) induced membrane depolarization associated with a decrease in membrane conductance in the majority (70%) of SPNs tested, via activation of postsynaptic H1 receptors negatively coupled to one or more unidentified K+ conductances. Histamine and HTMT application also induced or increased the amplitude and/or frequency of membrane potential oscillations in electrotonically coupled SPNs. The H2 receptor agonist dimaprit (10 microM) or the H3 receptor agonist imetit (100 nM) were without significant effect on the membrane properties of SPNs. Histamine responses were sensitive to the H1 receptor antagonist triprolidine (10 microM) and the nonselective potassium channel blocker barium (1 mM) but were unaffected by the H2 receptor antagonist tiotidine (10 microM) and the H3 receptor antagonist, clobenpropit (5 microM). Single cell RT-PCR revealed mRNA expression for H1 receptors in 75% of SPNs tested, with no expression of mRNA for H2, H3, or H4 receptors. These data represent the first demonstration of H1 receptor expression in SPNs and suggest that histamine acts to regulate excitability of these neurons via a direct postsynaptic effect on H1 receptors. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/16354729/Histamine_excites_neonatal_rat_sympathetic_preganglionic_neurons_in_vitro_via_activation_of_H1_receptors_ L2 - https://journals.physiology.org/doi/10.1152/jn.01135.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -