Tags

Type your tag names separated by a space and hit enter

Short report: application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis.
Am J Trop Med Hyg. 2005 Dec; 73(6):1162-4.AJ

Abstract

The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis.

Authors+Show Affiliations

Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16354831

Citation

Gal, Daniel, et al. "Short Report: Application of a Polymerase Chain Reaction to Detect Burkholderia Pseudomallei in Clinical Specimens From Patients With Suspected Melioidosis." The American Journal of Tropical Medicine and Hygiene, vol. 73, no. 6, 2005, pp. 1162-4.
Gal D, Mayo M, Spencer E, et al. Short report: application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. Am J Trop Med Hyg. 2005;73(6):1162-4.
Gal, D., Mayo, M., Spencer, E., Cheng, A. C., & Currie, B. J. (2005). Short report: application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. The American Journal of Tropical Medicine and Hygiene, 73(6), 1162-4.
Gal D, et al. Short Report: Application of a Polymerase Chain Reaction to Detect Burkholderia Pseudomallei in Clinical Specimens From Patients With Suspected Melioidosis. Am J Trop Med Hyg. 2005;73(6):1162-4. PubMed PMID: 16354831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Short report: application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. AU - Gal,Daniel, AU - Mayo,Mark, AU - Spencer,Emma, AU - Cheng,Allen C, AU - Currie,Bart J, PY - 2005/12/16/pubmed PY - 2006/6/21/medline PY - 2005/12/16/entrez SP - 1162 EP - 4 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 73 IS - 6 N2 - The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 18 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/16354831/Short_report:_application_of_a_polymerase_chain_reaction_to_detect_Burkholderia_pseudomallei_in_clinical_specimens_from_patients_with_suspected_melioidosis_ DB - PRIME DP - Unbound Medicine ER -