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Immune response induced by Salmonella typhimurium defective in ppGpp synthesis.
Vaccine. 2006 Mar 15; 24(12):2027-34.V

Abstract

Systemic infection by Salmonella typhimurium requires coordinated expression of virulence genes found primarily in Salmonella Pathogenecity Islands (SPIs). We have previously reported that the intracellular signal that induces these virulence genes is a stringent signal molecule, ppGpp [Song et al. J Biol Chem 2003;279:34183]. In this study, we found that relA and spoT double mutant Salmonella (DeltappGpp strain), which is defective in ppGpp synthesis, was virtually avirulent in BALB/c mice. Subsequently, the live vaccine potential of the avirulent DeltappGpp Salmonella strain was determined. A single immunization with live DeltappGpp Salmonella efficiently protected mice from challenge with wild-type Salmonella at a dose 10(6)-fold above the LD50 30 days after immunization. Various assays revealed that immunization of mice with the DeltappGpp strain elicited both systemic and mucosal antibody responses, in addition to cell-mediated immunity.

Authors+Show Affiliations

Genome Research Center for Enteropathogenic Bacteria and Research Institute of Vibrio Infection, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16356600

Citation

Na, Hee Sam, et al. "Immune Response Induced By Salmonella Typhimurium Defective in ppGpp Synthesis." Vaccine, vol. 24, no. 12, 2006, pp. 2027-34.
Na HS, Kim HJ, Lee HC, et al. Immune response induced by Salmonella typhimurium defective in ppGpp synthesis. Vaccine. 2006;24(12):2027-34.
Na, H. S., Kim, H. J., Lee, H. C., Hong, Y., Rhee, J. H., & Choy, H. E. (2006). Immune response induced by Salmonella typhimurium defective in ppGpp synthesis. Vaccine, 24(12), 2027-34.
Na HS, et al. Immune Response Induced By Salmonella Typhimurium Defective in ppGpp Synthesis. Vaccine. 2006 Mar 15;24(12):2027-34. PubMed PMID: 16356600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immune response induced by Salmonella typhimurium defective in ppGpp synthesis. AU - Na,Hee Sam, AU - Kim,Hyun Ju, AU - Lee,Hyun-Chul, AU - Hong,Yeongjin, AU - Rhee,Joon Haeng, AU - Choy,Hyon E, Y1 - 2005/12/01/ PY - 2005/08/26/received PY - 2005/11/09/revised PY - 2005/11/15/accepted PY - 2005/12/17/pubmed PY - 2006/6/29/medline PY - 2005/12/17/entrez SP - 2027 EP - 34 JF - Vaccine JO - Vaccine VL - 24 IS - 12 N2 - Systemic infection by Salmonella typhimurium requires coordinated expression of virulence genes found primarily in Salmonella Pathogenecity Islands (SPIs). We have previously reported that the intracellular signal that induces these virulence genes is a stringent signal molecule, ppGpp [Song et al. J Biol Chem 2003;279:34183]. In this study, we found that relA and spoT double mutant Salmonella (DeltappGpp strain), which is defective in ppGpp synthesis, was virtually avirulent in BALB/c mice. Subsequently, the live vaccine potential of the avirulent DeltappGpp Salmonella strain was determined. A single immunization with live DeltappGpp Salmonella efficiently protected mice from challenge with wild-type Salmonella at a dose 10(6)-fold above the LD50 30 days after immunization. Various assays revealed that immunization of mice with the DeltappGpp strain elicited both systemic and mucosal antibody responses, in addition to cell-mediated immunity. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/16356600/Immune_response_induced_by_Salmonella_typhimurium_defective_in_ppGpp_synthesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(05)01174-6 DB - PRIME DP - Unbound Medicine ER -