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Effect of Dracocephalum argunense on mast-cell-mediated hypersensitivity.
Int Arch Allergy Immunol. 2006; 139(2):87-95.IA

Abstract

BACKGROUND

Stimulation of mast cells starts the process of degranulation resulting in release of mediators such as histamine and an array of inflammatory cytokines. In this report, we investigated the effect of an aqueous extract of Dracocephalum argunense Fisch. (Labiatae) (DAAE) on immediate-type hypersensitivity and studied its possible mechanisms of action, focusing on histamine release and proinflammatory cytokine expression in mast cells.

METHODS

An in vivo model of systemic and local allergic reaction was investigated. Compound 48/80- or IgE-induced histamine release from mast cells was measured. The expression of proinflammatory cytokines such as TNF-alpha and IL-6 was measured by RT-PCR and ELISA. The level of intracellular calcium was measured by spectrofluorometry. NF-kappaB activation was measured by Western blot, EMSA and luciferase assay.

RESULTS

DAAE inhibited systemic anaphylaxis, local allergic reactions, and serum histamine release in a dose-dependent manner in mice. DAAE dose-dependently reduced histamine release from mast cells activated by compound 48/80 or IgE. The inhibitory effect of DAAE on histamine release was mediated by the modulation of intracellular calcium. In addition, DAAE decreased TNF-alpha and IL-6 gene expression and production in human mast cells stimulated by phorbol-12-myristate-13-acetate (PMA) plus calcium ionophore A23187. The inhibitory effect of DAAE on the TNF-alpha and IL-6 expression was NF-kappaB dependent.

CONCLUSION

Our findings provide evidence that DAAE inhibits mast-cell-derived immediate-type hypersensitivity. Taken together, the anti-allergic effect of DAAE in vivo and in vitro suggests a possible clinical use of this agent in immediate-type hypersensitivity.

Authors+Show Affiliations

Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16357489

Citation

Kim, Sang-Hyun, and Tae-Yong Shin. "Effect of Dracocephalum Argunense On Mast-cell-mediated Hypersensitivity." International Archives of Allergy and Immunology, vol. 139, no. 2, 2006, pp. 87-95.
Kim SH, Shin TY. Effect of Dracocephalum argunense on mast-cell-mediated hypersensitivity. Int Arch Allergy Immunol. 2006;139(2):87-95.
Kim, S. H., & Shin, T. Y. (2006). Effect of Dracocephalum argunense on mast-cell-mediated hypersensitivity. International Archives of Allergy and Immunology, 139(2), 87-95.
Kim SH, Shin TY. Effect of Dracocephalum Argunense On Mast-cell-mediated Hypersensitivity. Int Arch Allergy Immunol. 2006;139(2):87-95. PubMed PMID: 16357489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Dracocephalum argunense on mast-cell-mediated hypersensitivity. AU - Kim,Sang-Hyun, AU - Shin,Tae-Yong, Y1 - 2005/12/16/ PY - 2005/07/25/received PY - 2005/09/01/accepted PY - 2005/12/17/pubmed PY - 2006/3/16/medline PY - 2005/12/17/entrez SP - 87 EP - 95 JF - International archives of allergy and immunology JO - Int Arch Allergy Immunol VL - 139 IS - 2 N2 - BACKGROUND: Stimulation of mast cells starts the process of degranulation resulting in release of mediators such as histamine and an array of inflammatory cytokines. In this report, we investigated the effect of an aqueous extract of Dracocephalum argunense Fisch. (Labiatae) (DAAE) on immediate-type hypersensitivity and studied its possible mechanisms of action, focusing on histamine release and proinflammatory cytokine expression in mast cells. METHODS: An in vivo model of systemic and local allergic reaction was investigated. Compound 48/80- or IgE-induced histamine release from mast cells was measured. The expression of proinflammatory cytokines such as TNF-alpha and IL-6 was measured by RT-PCR and ELISA. The level of intracellular calcium was measured by spectrofluorometry. NF-kappaB activation was measured by Western blot, EMSA and luciferase assay. RESULTS: DAAE inhibited systemic anaphylaxis, local allergic reactions, and serum histamine release in a dose-dependent manner in mice. DAAE dose-dependently reduced histamine release from mast cells activated by compound 48/80 or IgE. The inhibitory effect of DAAE on histamine release was mediated by the modulation of intracellular calcium. In addition, DAAE decreased TNF-alpha and IL-6 gene expression and production in human mast cells stimulated by phorbol-12-myristate-13-acetate (PMA) plus calcium ionophore A23187. The inhibitory effect of DAAE on the TNF-alpha and IL-6 expression was NF-kappaB dependent. CONCLUSION: Our findings provide evidence that DAAE inhibits mast-cell-derived immediate-type hypersensitivity. Taken together, the anti-allergic effect of DAAE in vivo and in vitro suggests a possible clinical use of this agent in immediate-type hypersensitivity. SN - 1018-2438 UR - https://www.unboundmedicine.com/medline/citation/16357489/Effect_of_Dracocephalum_argunense_on_mast_cell_mediated_hypersensitivity_ L2 - https://www.karger.com?DOI=10.1159/000090383 DB - PRIME DP - Unbound Medicine ER -