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Optical fiber light-emitting diode-induced fluorescence detection for capillary electrophoresis.
Electrophoresis. 2006 Feb; 27(2):461-7.E

Abstract

A highly sensitive optical fiber light-emitting diode (LED)-induced fluorescence detector for CE has been constructed and evaluated. In this detector, a violet or blue LED was used as the excitation source and an optical fiber with 40 microm OD was used to transmit the excitation light. The upper end of the fiber was inserted into the separation capillary and was situated right at the detection window. Fluorescence emission was collected by a 40 x microscope objective, focused on a spatial filter, and passed through a cutoff filter before reaching the photomultiplier tube. Output signals were recorded and processed with a computer using in-house written software. The present CE/fluorescence detector deploys a simple and inexpensive optical system that requires only an LED as the light source. Its utility was successfully demonstrated by the separation and determination of amino acids (AAs) labeled with naphthalene-2,3-dicarboxaldehyde (NDA) and FITC. Low detection limits were obtained ranging from 17 to 23 nM for NDA-tagged AAs and 8 to 12 nM for FITC-labeled AAs (S/N=3). By virtue of such valuable features as low cost, convenience, and miniaturization, the presented detection scheme was proven to be attractive for sensitive fluorescence detection in CE.

Authors+Show Affiliations

Zhao S
College of Chemical Engineering, Sichuan University, Chengdu, China.
Yuan H
No affiliation info available
Xiao D
No affiliation info available

MeSH

Amino AcidsElectrophoresis, CapillaryFluorescein-5-isothiocyanateFluorometryLightNaphthalenesOptics and Photonics

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16358357

Citation

Zhao, Shulin, et al. "Optical Fiber Light-emitting Diode-induced Fluorescence Detection for Capillary Electrophoresis." Electrophoresis, vol. 27, no. 2, 2006, pp. 461-7.
Zhao S, Yuan H, Xiao D. Optical fiber light-emitting diode-induced fluorescence detection for capillary electrophoresis. Electrophoresis. 2006;27(2):461-7.
Zhao, S., Yuan, H., & Xiao, D. (2006). Optical fiber light-emitting diode-induced fluorescence detection for capillary electrophoresis. Electrophoresis, 27(2), 461-7.
Zhao S, Yuan H, Xiao D. Optical Fiber Light-emitting Diode-induced Fluorescence Detection for Capillary Electrophoresis. Electrophoresis. 2006;27(2):461-7. PubMed PMID: 16358357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optical fiber light-emitting diode-induced fluorescence detection for capillary electrophoresis. AU - Zhao,Shulin, AU - Yuan,Hongyan, AU - Xiao,Dan, PY - 2005/12/17/pubmed PY - 2006/3/18/medline PY - 2005/12/17/entrez SP - 461 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 2 N2 - A highly sensitive optical fiber light-emitting diode (LED)-induced fluorescence detector for CE has been constructed and evaluated. In this detector, a violet or blue LED was used as the excitation source and an optical fiber with 40 microm OD was used to transmit the excitation light. The upper end of the fiber was inserted into the separation capillary and was situated right at the detection window. Fluorescence emission was collected by a 40 x microscope objective, focused on a spatial filter, and passed through a cutoff filter before reaching the photomultiplier tube. Output signals were recorded and processed with a computer using in-house written software. The present CE/fluorescence detector deploys a simple and inexpensive optical system that requires only an LED as the light source. Its utility was successfully demonstrated by the separation and determination of amino acids (AAs) labeled with naphthalene-2,3-dicarboxaldehyde (NDA) and FITC. Low detection limits were obtained ranging from 17 to 23 nM for NDA-tagged AAs and 8 to 12 nM for FITC-labeled AAs (S/N=3). By virtue of such valuable features as low cost, convenience, and miniaturization, the presented detection scheme was proven to be attractive for sensitive fluorescence detection in CE. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16358357/Optical_fiber_light_emitting_diode_induced_fluorescence_detection_for_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -