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Presynaptic low- and high-affinity kainate receptors in nociceptive spinal afferents.
Pain. 2006 Jan; 120(1-2):97-105.PAIN

Abstract

Presynaptic ionotropic glutamate receptors are increasingly attributed a role in the modulation of sensory input at the first synapse of dorsal root ganglion (DRG) neurons in the spinal dorsal horn. Central terminals of DRG neurons express AMPA and NMDA receptors whose activation modulates the release of glutamate, the main transmitter at these synapses. Previous work, with an antibody that recognizes all low-affinity kainate receptor subunits (GluR5, 6, 7), provided microscopic evidence of presynaptic kainate receptors in unidentified primary afferent terminals in superficial laminae of the spinal dorsal horn (Hwang SJ, Pagliardini S, Rustioni A, Valtschanoff JG. Presynaptic kainate receptors in primary afferents to the superficial laminae of the rat spinal cord. J Comp Neurol 2001; 436: pp. 275-289). We show here that, although all such subunits may be expressed in these terminals, GluR5 is the subunit most readily detectable at presynaptic sites in sections processed for immunocytochemistry. We also show that the high-affinity kainate receptor subunits KA1 and KA2 are expressed in central terminals of DRG neurons and are co-expressed with low-affinity receptor subunits in the same terminals. Quantitative data show that kainate-expressing DRG neurons are about six times more likely to express the P2X(3) subunit of the purinergic receptor than to express substance P. Thus, nociceptive afferents that express presynaptic kainate receptors are predominantly non-peptidergic, suggesting a role for these receptors in the modulation of neuropathic rather than inflammatory pain.

Authors+Show Affiliations

Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 27599, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16360275

Citation

Lucifora, Simona, et al. "Presynaptic Low- and High-affinity Kainate Receptors in Nociceptive Spinal Afferents." Pain, vol. 120, no. 1-2, 2006, pp. 97-105.
Lucifora S, Willcockson HH, Lu CR, et al. Presynaptic low- and high-affinity kainate receptors in nociceptive spinal afferents. Pain. 2006;120(1-2):97-105.
Lucifora, S., Willcockson, H. H., Lu, C. R., Darstein, M., Phend, K. D., Valtschanoff, J. G., & Rustioni, A. (2006). Presynaptic low- and high-affinity kainate receptors in nociceptive spinal afferents. Pain, 120(1-2), 97-105.
Lucifora S, et al. Presynaptic Low- and High-affinity Kainate Receptors in Nociceptive Spinal Afferents. Pain. 2006;120(1-2):97-105. PubMed PMID: 16360275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presynaptic low- and high-affinity kainate receptors in nociceptive spinal afferents. AU - Lucifora,Simona, AU - Willcockson,Helen H, AU - Lu,Chun-Rong, AU - Darstein,Melanie, AU - Phend,Kris D, AU - Valtschanoff,Juli G, AU - Rustioni,Aldo, Y1 - 2005/12/15/ PY - 2005/07/21/received PY - 2005/09/26/revised PY - 2005/10/19/accepted PY - 2005/12/20/pubmed PY - 2006/3/31/medline PY - 2005/12/20/entrez SP - 97 EP - 105 JF - Pain JO - Pain VL - 120 IS - 1-2 N2 - Presynaptic ionotropic glutamate receptors are increasingly attributed a role in the modulation of sensory input at the first synapse of dorsal root ganglion (DRG) neurons in the spinal dorsal horn. Central terminals of DRG neurons express AMPA and NMDA receptors whose activation modulates the release of glutamate, the main transmitter at these synapses. Previous work, with an antibody that recognizes all low-affinity kainate receptor subunits (GluR5, 6, 7), provided microscopic evidence of presynaptic kainate receptors in unidentified primary afferent terminals in superficial laminae of the spinal dorsal horn (Hwang SJ, Pagliardini S, Rustioni A, Valtschanoff JG. Presynaptic kainate receptors in primary afferents to the superficial laminae of the rat spinal cord. J Comp Neurol 2001; 436: pp. 275-289). We show here that, although all such subunits may be expressed in these terminals, GluR5 is the subunit most readily detectable at presynaptic sites in sections processed for immunocytochemistry. We also show that the high-affinity kainate receptor subunits KA1 and KA2 are expressed in central terminals of DRG neurons and are co-expressed with low-affinity receptor subunits in the same terminals. Quantitative data show that kainate-expressing DRG neurons are about six times more likely to express the P2X(3) subunit of the purinergic receptor than to express substance P. Thus, nociceptive afferents that express presynaptic kainate receptors are predominantly non-peptidergic, suggesting a role for these receptors in the modulation of neuropathic rather than inflammatory pain. SN - 0304-3959 UR - https://www.unboundmedicine.com/medline/citation/16360275/Presynaptic_low__and_high_affinity_kainate_receptors_in_nociceptive_spinal_afferents_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3959(05)00533-6 DB - PRIME DP - Unbound Medicine ER -