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Screening the allergenic repertoires of wheat and maize with sera from double-blind, placebo-controlled food challenge positive patients.
Allergy 2006; 61(1):128-35A

Abstract

BACKGROUND

Food allergy to wheat and maize is an increasing factor of deterioration of life quality, especially childhood and can, in rare cases, even induce anaphylaxis. Although omega-5 gliadin from wheat and maize lipid transfer protein have been characterized as major cereal allergens on the molecular level, the list of food allergens is far to be complete.

METHODS

To identify the IgE-binding repertoires of wheat and maize we screened respective cDNA libraries displayed on phage surface with sera from patients with a confirmed food allergy. The study included six patients with a positive double-blind, placebo-controlled food challenge (DBPCFC) to wheat, nine patients with a positive DBPCFC to maize, and six patients with anaphylactic reactions after ingestion of wheat.

RESULTS

The enriched sequences encoding IgE-binding proteins showed heterogeneous repertoires for both, wheat and maize. The selected wheat repertoire yielded 12, the maize repertoire 11 open reading frames. Among these we identified allergens belonging to already characterized allergens families, such as gliadin, profilin and beta-expansin. Besides, we found novel proteins with high cross-reactive potential, such as thioredoxins, as well as sequences that had so far not been related to cereal allergy at all. The IgE-binding capacity of some selected proteins was evaluated in vitro and cross-reactivity was demonstrated by competition ELISA.

CONCLUSION

With regard to the heterogeneity of the characterized sequences as well as to the biochemical nature of the new allergens detected we conclude that wheat and maize-related food allergy is more complex than so far anticipated.

Authors+Show Affiliations

Swiss Institute of Allergy and Asthma Research (SIAF), Davos Platz, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16364168

Citation

Weichel, M, et al. "Screening the Allergenic Repertoires of Wheat and Maize With Sera From Double-blind, Placebo-controlled Food Challenge Positive Patients." Allergy, vol. 61, no. 1, 2006, pp. 128-35.
Weichel M, Vergoossen NJ, Bonomi S, et al. Screening the allergenic repertoires of wheat and maize with sera from double-blind, placebo-controlled food challenge positive patients. Allergy. 2006;61(1):128-35.
Weichel, M., Vergoossen, N. J., Bonomi, S., Scibilia, J., Ortolani, C., Ballmer-Weber, B. K., ... Crameri, R. (2006). Screening the allergenic repertoires of wheat and maize with sera from double-blind, placebo-controlled food challenge positive patients. Allergy, 61(1), pp. 128-35.
Weichel M, et al. Screening the Allergenic Repertoires of Wheat and Maize With Sera From Double-blind, Placebo-controlled Food Challenge Positive Patients. Allergy. 2006;61(1):128-35. PubMed PMID: 16364168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening the allergenic repertoires of wheat and maize with sera from double-blind, placebo-controlled food challenge positive patients. AU - Weichel,M, AU - Vergoossen,N J, AU - Bonomi,S, AU - Scibilia,J, AU - Ortolani,C, AU - Ballmer-Weber,B K, AU - Pastorello,E A, AU - Crameri,R, PY - 2005/12/21/pubmed PY - 2006/6/8/medline PY - 2005/12/21/entrez SP - 128 EP - 35 JF - Allergy JO - Allergy VL - 61 IS - 1 N2 - BACKGROUND: Food allergy to wheat and maize is an increasing factor of deterioration of life quality, especially childhood and can, in rare cases, even induce anaphylaxis. Although omega-5 gliadin from wheat and maize lipid transfer protein have been characterized as major cereal allergens on the molecular level, the list of food allergens is far to be complete. METHODS: To identify the IgE-binding repertoires of wheat and maize we screened respective cDNA libraries displayed on phage surface with sera from patients with a confirmed food allergy. The study included six patients with a positive double-blind, placebo-controlled food challenge (DBPCFC) to wheat, nine patients with a positive DBPCFC to maize, and six patients with anaphylactic reactions after ingestion of wheat. RESULTS: The enriched sequences encoding IgE-binding proteins showed heterogeneous repertoires for both, wheat and maize. The selected wheat repertoire yielded 12, the maize repertoire 11 open reading frames. Among these we identified allergens belonging to already characterized allergens families, such as gliadin, profilin and beta-expansin. Besides, we found novel proteins with high cross-reactive potential, such as thioredoxins, as well as sequences that had so far not been related to cereal allergy at all. The IgE-binding capacity of some selected proteins was evaluated in vitro and cross-reactivity was demonstrated by competition ELISA. CONCLUSION: With regard to the heterogeneity of the characterized sequences as well as to the biochemical nature of the new allergens detected we conclude that wheat and maize-related food allergy is more complex than so far anticipated. SN - 0105-4538 UR - https://www.unboundmedicine.com/medline/citation/16364168/Screening_the_allergenic_repertoires_of_wheat_and_maize_with_sera_from_double_blind_placebo_controlled_food_challenge_positive_patients_ L2 - https://doi.org/10.1111/j.1398-9995.2006.00999.x DB - PRIME DP - Unbound Medicine ER -