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Preparative isolation and purification of four compounds from the Chinese medicinal herb rhizoma Anemarrhenae by high-speed counter-current chromatography.
J Chromatogr A. 2006 Feb 03; 1104(1-2):69-74.JC

Abstract

High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of mangiferin, neomangiferin, cis-hinkiresinol and (-)-4'-O-methylnyasol from the Chinese medicinal herb rhizoma Anemarrhenae. Five hundred milligrams of crude extracts were separated by using n-butanol-acetic acid (1%) (1:1, v/v) as the two-phase solvent system and yielded 35.3 mg of neomangiferin and 245.4 mg of mangiferin. During this separation, cis-hinkiresinol and (-)-4'-O-methylnyasol were still maintained in the stationary phase. The stationary phase was collected, evaporated to dryness and separated with light petroleum-ethyl acetate-methanol-water (1:1:1.2:0.8, v/v) and 1:1:1.4:0.6 (v/v) in gradient elution, which yielded 17.2 mg of cis-hinkiresinol and 12.4 mg of (-)-4'-O-methylnyasol. The purities of mangiferin, neomangiferin, cis-hinkiresinol and (-)-4'-O-methylnyasol were 96.3, 98.0, 97.3 and 98.2%, respectively, as determined by HPLC. The chemical structures of these components were identified by 1H NMR and 13C NMR.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16364341

Citation

Sun, Qinghua, et al. "Preparative Isolation and Purification of Four Compounds From the Chinese Medicinal Herb Rhizoma Anemarrhenae By High-speed Counter-current Chromatography." Journal of Chromatography. A, vol. 1104, no. 1-2, 2006, pp. 69-74.
Sun Q, Sun A, Liu R. Preparative isolation and purification of four compounds from the Chinese medicinal herb rhizoma Anemarrhenae by high-speed counter-current chromatography. J Chromatogr A. 2006;1104(1-2):69-74.
Sun, Q., Sun, A., & Liu, R. (2006). Preparative isolation and purification of four compounds from the Chinese medicinal herb rhizoma Anemarrhenae by high-speed counter-current chromatography. Journal of Chromatography. A, 1104(1-2), 69-74.
Sun Q, Sun A, Liu R. Preparative Isolation and Purification of Four Compounds From the Chinese Medicinal Herb Rhizoma Anemarrhenae By High-speed Counter-current Chromatography. J Chromatogr A. 2006 Feb 3;1104(1-2):69-74. PubMed PMID: 16364341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative isolation and purification of four compounds from the Chinese medicinal herb rhizoma Anemarrhenae by high-speed counter-current chromatography. AU - Sun,Qinghua, AU - Sun,Ailing, AU - Liu,Renmin, Y1 - 2005/12/20/ PY - 2005/10/06/received PY - 2005/11/10/revised PY - 2005/11/15/accepted PY - 2005/12/21/pubmed PY - 2006/4/7/medline PY - 2005/12/21/entrez SP - 69 EP - 74 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1104 IS - 1-2 N2 - High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of mangiferin, neomangiferin, cis-hinkiresinol and (-)-4'-O-methylnyasol from the Chinese medicinal herb rhizoma Anemarrhenae. Five hundred milligrams of crude extracts were separated by using n-butanol-acetic acid (1%) (1:1, v/v) as the two-phase solvent system and yielded 35.3 mg of neomangiferin and 245.4 mg of mangiferin. During this separation, cis-hinkiresinol and (-)-4'-O-methylnyasol were still maintained in the stationary phase. The stationary phase was collected, evaporated to dryness and separated with light petroleum-ethyl acetate-methanol-water (1:1:1.2:0.8, v/v) and 1:1:1.4:0.6 (v/v) in gradient elution, which yielded 17.2 mg of cis-hinkiresinol and 12.4 mg of (-)-4'-O-methylnyasol. The purities of mangiferin, neomangiferin, cis-hinkiresinol and (-)-4'-O-methylnyasol were 96.3, 98.0, 97.3 and 98.2%, respectively, as determined by HPLC. The chemical structures of these components were identified by 1H NMR and 13C NMR. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16364341/Preparative_isolation_and_purification_of_four_compounds_from_the_Chinese_medicinal_herb_rhizoma_Anemarrhenae_by_high_speed_counter_current_chromatography_ DB - PRIME DP - Unbound Medicine ER -