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Analysis of porcine differential gene expression following challenge with Salmonella enterica serovar Choleraesuis using suppression subtractive hybridization.
Vet Microbiol. 2006 Apr 16; 114(1-2):60-71.VM

Abstract

Swine-adapted Salmonella enterica subsp. enterica serovar Choleraesuis (S. Choleraesuis) is the pathogen most frequently isolated from diseased pigs and may affect host gene expression in a species-specific manner. To characterize the porcine transcriptional response to S. Choleraesuis infection, the mRNA profiles from the mesenteric lymph nodes of three non-infected and three experimentally infected pigs at 24 h post-inoculation were analyzed by suppression subtractive hybridization (SSH). Forty-four up-regulated and 44 down-regulated genes were revealed by differential cDNA screening of 384 forward and 288 reverse subtracted cDNA clones. The DNA sequence of the cDNA clones identified genes with a role in a variety of cellular functions as well as gene products of unknown function. Seven up-regulated genes (CXCL10, CXCR4, SDCBP, DNAJA1, HSPH1, HSP90 and ANXA5) and two functionally related genes (HSP70 and DNAJA4:pDJA1) were selected for further analysis based on their predicted roles in infection and immunity. Real-time RT-PCR was performed using RNA collected from a time course of infection spanning from the acute phase (8 h) to the chronic phase (21 days) to confirm and quantitate the up-regulation of the SSH-enriched genes. Correlating with the clinical signs of infection (fever, diarrhea and lethargy), the most dramatic induction of gene expression for all nine genes occurred at 48 h post-inoculation. This investigation further defines the porcine response to a host-adapted strain of Salmonella by revealing the differential expression of genes with a role in a variety of host cellular functions including innate immunity and cytoskeleton regulation.

Authors+Show Affiliations

Pre-harvest Food Safety and Enteric Diseases Research Unit, USDA, ARS, National Animal Disease Center, Ames, IA 50010, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16364571

Citation

Uthe, Jolita J., et al. "Analysis of Porcine Differential Gene Expression Following Challenge With Salmonella Enterica Serovar Choleraesuis Using Suppression Subtractive Hybridization." Veterinary Microbiology, vol. 114, no. 1-2, 2006, pp. 60-71.
Uthe JJ, Stabel TJ, Zhao SH, et al. Analysis of porcine differential gene expression following challenge with Salmonella enterica serovar Choleraesuis using suppression subtractive hybridization. Vet Microbiol. 2006;114(1-2):60-71.
Uthe, J. J., Stabel, T. J., Zhao, S. H., Tuggle, C. K., & Bearson, S. M. (2006). Analysis of porcine differential gene expression following challenge with Salmonella enterica serovar Choleraesuis using suppression subtractive hybridization. Veterinary Microbiology, 114(1-2), 60-71.
Uthe JJ, et al. Analysis of Porcine Differential Gene Expression Following Challenge With Salmonella Enterica Serovar Choleraesuis Using Suppression Subtractive Hybridization. Vet Microbiol. 2006 Apr 16;114(1-2):60-71. PubMed PMID: 16364571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of porcine differential gene expression following challenge with Salmonella enterica serovar Choleraesuis using suppression subtractive hybridization. AU - Uthe,Jolita J, AU - Stabel,Thomas J, AU - Zhao,Shu-hong, AU - Tuggle,Christopher K, AU - Bearson,Shawn M D, Y1 - 2005/12/20/ PY - 2005/06/24/received PY - 2005/10/26/revised PY - 2005/10/28/accepted PY - 2005/12/21/pubmed PY - 2007/6/6/medline PY - 2005/12/21/entrez SP - 60 EP - 71 JF - Veterinary microbiology JO - Vet Microbiol VL - 114 IS - 1-2 N2 - Swine-adapted Salmonella enterica subsp. enterica serovar Choleraesuis (S. Choleraesuis) is the pathogen most frequently isolated from diseased pigs and may affect host gene expression in a species-specific manner. To characterize the porcine transcriptional response to S. Choleraesuis infection, the mRNA profiles from the mesenteric lymph nodes of three non-infected and three experimentally infected pigs at 24 h post-inoculation were analyzed by suppression subtractive hybridization (SSH). Forty-four up-regulated and 44 down-regulated genes were revealed by differential cDNA screening of 384 forward and 288 reverse subtracted cDNA clones. The DNA sequence of the cDNA clones identified genes with a role in a variety of cellular functions as well as gene products of unknown function. Seven up-regulated genes (CXCL10, CXCR4, SDCBP, DNAJA1, HSPH1, HSP90 and ANXA5) and two functionally related genes (HSP70 and DNAJA4:pDJA1) were selected for further analysis based on their predicted roles in infection and immunity. Real-time RT-PCR was performed using RNA collected from a time course of infection spanning from the acute phase (8 h) to the chronic phase (21 days) to confirm and quantitate the up-regulation of the SSH-enriched genes. Correlating with the clinical signs of infection (fever, diarrhea and lethargy), the most dramatic induction of gene expression for all nine genes occurred at 48 h post-inoculation. This investigation further defines the porcine response to a host-adapted strain of Salmonella by revealing the differential expression of genes with a role in a variety of host cellular functions including innate immunity and cytoskeleton regulation. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/16364571/Analysis_of_porcine_differential_gene_expression_following_challenge_with_Salmonella_enterica_serovar_Choleraesuis_using_suppression_subtractive_hybridization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(05)00415-3 DB - PRIME DP - Unbound Medicine ER -