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Phenolic antioxidants (hydrolyzable tannins, flavonols, and anthocyanins) identified by LC-ESI-MS and MALDI-QIT-TOF MS from Rosa chinensis flowers.
J Agric Food Chem. 2005 Dec 28; 53(26):9940-8.JA

Abstract

Rosa chinensis (Yuejihua) is a well-known ornamental plant, and its flowers are commonly used in traditional Chinese medicine. Methanolic crude extracts of dried R. chinensis flowers were used for simultaneous determination of phenolic constituents by liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS). A total of 36 known and unknown phenolics were identified as hydrolyzable tannins, flavonols, and anthocyanins, mainly including gallotannins (mono-, di-, or trigalloylglucopyranosides), ellagitannins, quercetin, quercetin/kaempferol mono- and diglycosides, and cyanidin/pelargonidin diglycosides. MALDI-QIT-TOF MS was applied not only to verify most phenolics isolated and identified by LC-MS but also to tentatively identify two ellagitannins (rugosins B and C) not isolated and unidentified by LC-MS. This study is the first to demonstrate the rapid and successful use of MALDI-QIT-TOF MS and LC-MS to directly and simultaneously identify phenolics in the crude extracts of R. chinensis flowers without any purification. The antioxidant activity of the crude extracts from R. chinensis flowers was also measured with three assay methods. The results showed that the phenolic antioxidants from R. chinensis flowers exhibited very strong radical scavenging effect and antioxidant power. High levels of flavonols and hydrolyzable tannins might be important bioactive principles in the dried R. chinensis flowers.

Authors+Show Affiliations

Cai YZ
Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong.
Xing J
No affiliation info available
Sun M
No affiliation info available
Zhan ZQ
No affiliation info available
Corke H
No affiliation info available

MeSH

AnthocyaninsAntioxidantsBiphenyl CompoundsFerric CompoundsFlavonolsFlowersFree Radical ScavengersIndicators and ReagentsOxidation-ReductionPicratesPlant ExtractsRosaSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrophotometry, UltravioletTannins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16366678

Citation

Cai, Yi-Zhong, et al. "Phenolic Antioxidants (hydrolyzable Tannins, Flavonols, and Anthocyanins) Identified By LC-ESI-MS and MALDI-QIT-TOF MS From Rosa Chinensis Flowers." Journal of Agricultural and Food Chemistry, vol. 53, no. 26, 2005, pp. 9940-8.
Cai YZ, Xing J, Sun M, et al. Phenolic antioxidants (hydrolyzable tannins, flavonols, and anthocyanins) identified by LC-ESI-MS and MALDI-QIT-TOF MS from Rosa chinensis flowers. J Agric Food Chem. 2005;53(26):9940-8.
Cai, Y. Z., Xing, J., Sun, M., Zhan, Z. Q., & Corke, H. (2005). Phenolic antioxidants (hydrolyzable tannins, flavonols, and anthocyanins) identified by LC-ESI-MS and MALDI-QIT-TOF MS from Rosa chinensis flowers. Journal of Agricultural and Food Chemistry, 53(26), 9940-8.
Cai YZ, et al. Phenolic Antioxidants (hydrolyzable Tannins, Flavonols, and Anthocyanins) Identified By LC-ESI-MS and MALDI-QIT-TOF MS From Rosa Chinensis Flowers. J Agric Food Chem. 2005 Dec 28;53(26):9940-8. PubMed PMID: 16366678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenolic antioxidants (hydrolyzable tannins, flavonols, and anthocyanins) identified by LC-ESI-MS and MALDI-QIT-TOF MS from Rosa chinensis flowers. AU - Cai,Yi-Zhong, AU - Xing,Jie, AU - Sun,Mei, AU - Zhan,Zhao-Qi, AU - Corke,Harold, PY - 2005/12/22/pubmed PY - 2006/2/4/medline PY - 2005/12/22/entrez SP - 9940 EP - 8 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 26 N2 - Rosa chinensis (Yuejihua) is a well-known ornamental plant, and its flowers are commonly used in traditional Chinese medicine. Methanolic crude extracts of dried R. chinensis flowers were used for simultaneous determination of phenolic constituents by liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS). A total of 36 known and unknown phenolics were identified as hydrolyzable tannins, flavonols, and anthocyanins, mainly including gallotannins (mono-, di-, or trigalloylglucopyranosides), ellagitannins, quercetin, quercetin/kaempferol mono- and diglycosides, and cyanidin/pelargonidin diglycosides. MALDI-QIT-TOF MS was applied not only to verify most phenolics isolated and identified by LC-MS but also to tentatively identify two ellagitannins (rugosins B and C) not isolated and unidentified by LC-MS. This study is the first to demonstrate the rapid and successful use of MALDI-QIT-TOF MS and LC-MS to directly and simultaneously identify phenolics in the crude extracts of R. chinensis flowers without any purification. The antioxidant activity of the crude extracts from R. chinensis flowers was also measured with three assay methods. The results showed that the phenolic antioxidants from R. chinensis flowers exhibited very strong radical scavenging effect and antioxidant power. High levels of flavonols and hydrolyzable tannins might be important bioactive principles in the dried R. chinensis flowers. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16366678/Phenolic_antioxidants__hydrolyzable_tannins_flavonols_and_anthocyanins__identified_by_LC_ESI_MS_and_MALDI_QIT_TOF_MS_from_Rosa_chinensis_flowers_ DB - PRIME DP - Unbound Medicine ER -
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