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Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry.
Ecotoxicol Environ Saf. 2007 Jan; 66(1):49-56.EE

Abstract

This study was conducted to determine the role of algal-algal interactions in a multispecies microalgal population on their sensitivities to copper based on an enzyme inhibition assay using flow cytometric measures. Autofluorescence (chlorophyll a and phycocyanin) was used to identify species and count algal signals. The effect of multispecies population on copper toxicity of Microcystis aeruginousa was detected (1) at the same initial cell density, (2) at the same surface area, and (3) in the presence and absence of Chlorella pyrenoidosa and Scenedesmus obliquus. As copper concentrations increased, esterase activity of M. aeruginosa changed in a concentration-dependent manner. The 24 h EC(50) value of M. aeruginosa in the multispecies population was significantly (P < 0.05) higher than those in the single-species population. Compared with S. obliquus, the effect of C. pyrenoidosa on M. aeruginosa was more marked (the 24 h EC(50) value of copper on fluorescin diacetate fluorescence of M. aeruginosa was 11 microg/L). At 48 h copper exposure (6 microg/L) analysis of intracellular reactive oxygen species levels also showed similar algal-algal interactions in multispecies microalgal populations. The pigment assay suggested that these algal-algal interactions occurred only at low concentrations (< 13 microg/L, 24 and 48 h copper exposure). This study demonstrates the importance of using multispecies populations to estimate metal toxicity in natural waters.

Authors+Show Affiliations

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16368143

Citation

Yu, Yang, et al. "Determination of Short-term Copper Toxicity in a Multispecies Microalgal Population Using Flow Cytometry." Ecotoxicology and Environmental Safety, vol. 66, no. 1, 2007, pp. 49-56.
Yu Y, Kong F, Wang M, et al. Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry. Ecotoxicol Environ Saf. 2007;66(1):49-56.
Yu, Y., Kong, F., Wang, M., Qian, L., & Shi, X. (2007). Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry. Ecotoxicology and Environmental Safety, 66(1), 49-56.
Yu Y, et al. Determination of Short-term Copper Toxicity in a Multispecies Microalgal Population Using Flow Cytometry. Ecotoxicol Environ Saf. 2007;66(1):49-56. PubMed PMID: 16368143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry. AU - Yu,Yang, AU - Kong,Fanxiang, AU - Wang,Meilin, AU - Qian,Leilei, AU - Shi,Xiaoli, Y1 - 2005/12/20/ PY - 2005/02/23/received PY - 2005/10/10/revised PY - 2005/10/15/accepted PY - 2005/12/22/pubmed PY - 2007/3/1/medline PY - 2005/12/22/entrez SP - 49 EP - 56 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 66 IS - 1 N2 - This study was conducted to determine the role of algal-algal interactions in a multispecies microalgal population on their sensitivities to copper based on an enzyme inhibition assay using flow cytometric measures. Autofluorescence (chlorophyll a and phycocyanin) was used to identify species and count algal signals. The effect of multispecies population on copper toxicity of Microcystis aeruginousa was detected (1) at the same initial cell density, (2) at the same surface area, and (3) in the presence and absence of Chlorella pyrenoidosa and Scenedesmus obliquus. As copper concentrations increased, esterase activity of M. aeruginosa changed in a concentration-dependent manner. The 24 h EC(50) value of M. aeruginosa in the multispecies population was significantly (P < 0.05) higher than those in the single-species population. Compared with S. obliquus, the effect of C. pyrenoidosa on M. aeruginosa was more marked (the 24 h EC(50) value of copper on fluorescin diacetate fluorescence of M. aeruginosa was 11 microg/L). At 48 h copper exposure (6 microg/L) analysis of intracellular reactive oxygen species levels also showed similar algal-algal interactions in multispecies microalgal populations. The pigment assay suggested that these algal-algal interactions occurred only at low concentrations (< 13 microg/L, 24 and 48 h copper exposure). This study demonstrates the importance of using multispecies populations to estimate metal toxicity in natural waters. SN - 0147-6513 UR - https://www.unboundmedicine.com/medline/citation/16368143/Determination_of_short_term_copper_toxicity_in_a_multispecies_microalgal_population_using_flow_cytometry_ DB - PRIME DP - Unbound Medicine ER -