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NKp46 and NKG2D receptor expression in NK cells with CD56dim and CD56bright phenotype: regulation by histamine and reactive oxygen species.
Br J Haematol 2006; 132(1):91-8BJ

Abstract

The cytotoxicity of natural killer (NK) cells is dependent on the interaction between target cell ligands and a series of stimulatory receptors on NK cells. Two of these triggering receptors, the NKp46 natural cytotoxicity receptor (NKp46) and the major histocompatibility complex (MHC) class I-interactive NKG2D receptor, are deficiently expressed by NK cells recovered from patients with acute myeloid leukaemia (AML), but little is known regarding the regulation of NKp46 and NKG2D expression. Here we report that mononuclear and polymorphonuclear phagocytes downregulate the cell surface density of NKp46 and NKG2D on NK cells with CD56(dim) phenotype in vitro by a mechanism that is dependent on the availability of phagocyte-derived reactive oxygen species (ROS). Histamine maintained NKp46 and NKG2D expression despite the presence of inhibitory phagocytes by targeting an H2 receptor on phagocytes. By contrast, NKp46 and NKG2D expression by the CD56(bright) subset of NK cells was resistant to inhibition by phagocytes. Our findings are suggestive of a novel mechanism of relevance to the regulation of NKp46/NKG2D receptor expression. Moreover, our findings suggest that the previously reported action of histamine on NK cell-mediated killing of leukaemic cells may be related to the preservation of activatory NK-cell receptors.

Authors+Show Affiliations

Department of Virology, Göteborg University, Gothenburg, Sweden. ana.romero@microbio.gu.seNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16371024

Citation

Romero, Ana I., et al. "NKp46 and NKG2D Receptor Expression in NK Cells With CD56dim and CD56bright Phenotype: Regulation By Histamine and Reactive Oxygen Species." British Journal of Haematology, vol. 132, no. 1, 2006, pp. 91-8.
Romero AI, Thorén FB, Brune M, et al. NKp46 and NKG2D receptor expression in NK cells with CD56dim and CD56bright phenotype: regulation by histamine and reactive oxygen species. Br J Haematol. 2006;132(1):91-8.
Romero, A. I., Thorén, F. B., Brune, M., & Hellstrand, K. (2006). NKp46 and NKG2D receptor expression in NK cells with CD56dim and CD56bright phenotype: regulation by histamine and reactive oxygen species. British Journal of Haematology, 132(1), pp. 91-8.
Romero AI, et al. NKp46 and NKG2D Receptor Expression in NK Cells With CD56dim and CD56bright Phenotype: Regulation By Histamine and Reactive Oxygen Species. Br J Haematol. 2006;132(1):91-8. PubMed PMID: 16371024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NKp46 and NKG2D receptor expression in NK cells with CD56dim and CD56bright phenotype: regulation by histamine and reactive oxygen species. AU - Romero,Ana I, AU - Thorén,Fredrik B, AU - Brune,Mats, AU - Hellstrand,Kristoffer, PY - 2005/12/24/pubmed PY - 2006/1/18/medline PY - 2005/12/24/entrez SP - 91 EP - 8 JF - British journal of haematology JO - Br. J. Haematol. VL - 132 IS - 1 N2 - The cytotoxicity of natural killer (NK) cells is dependent on the interaction between target cell ligands and a series of stimulatory receptors on NK cells. Two of these triggering receptors, the NKp46 natural cytotoxicity receptor (NKp46) and the major histocompatibility complex (MHC) class I-interactive NKG2D receptor, are deficiently expressed by NK cells recovered from patients with acute myeloid leukaemia (AML), but little is known regarding the regulation of NKp46 and NKG2D expression. Here we report that mononuclear and polymorphonuclear phagocytes downregulate the cell surface density of NKp46 and NKG2D on NK cells with CD56(dim) phenotype in vitro by a mechanism that is dependent on the availability of phagocyte-derived reactive oxygen species (ROS). Histamine maintained NKp46 and NKG2D expression despite the presence of inhibitory phagocytes by targeting an H2 receptor on phagocytes. By contrast, NKp46 and NKG2D expression by the CD56(bright) subset of NK cells was resistant to inhibition by phagocytes. Our findings are suggestive of a novel mechanism of relevance to the regulation of NKp46/NKG2D receptor expression. Moreover, our findings suggest that the previously reported action of histamine on NK cell-mediated killing of leukaemic cells may be related to the preservation of activatory NK-cell receptors. SN - 0007-1048 UR - https://www.unboundmedicine.com/medline/citation/16371024/NKp46_and_NKG2D_receptor_expression_in_NK_cells_with_CD56dim_and_CD56bright_phenotype:_regulation_by_histamine_and_reactive_oxygen_species_ L2 - https://doi.org/10.1111/j.1365-2141.2005.05842.x DB - PRIME DP - Unbound Medicine ER -