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Red blood cells, platelets and polymorphonuclear neutrophils of patients with sickle cell disease exhibit oxidative stress that can be ameliorated by antioxidants.

Abstract

Sickle cell disease (SCD) is basically a red blood cell (RBC) disorder characterised by sickling and haemolysis, but platelets and polymorphonuclear neutrophils (PMN) are also involved. Oxidative damage may play a role in the pathogenesis of SCD. Using flow cytometry, we measured oxidative-state markers simultaneously in RBC, platelets and PMN obtained from 25 normal donors, nine homozygous (SS) patients and six SS/beta-thalassaemia patients. Reactive oxygen species (ROS) and reduced glutathione (GSH) were measured following staining of blood samples with fluorescence probes and gating on specific subpopulations based on size and granularity. Ten- to 30-fold higher ROS production and 20-50% lower GSH content were found in RBC, platelets and PMN from SCD patients versus those of their normal counterparts. This could in part account for the clinical manifestations, such as haemolysis, a hypercoagulable state, recurrent bacterial infections and vaso-occlusive incidences, in SCD. We further showed that exposure of SCD samples to antioxidants, such as N-acetyl-cysteine, vitamin C and vitamin E, decreased their oxidative stress. These results suggest that antioxidant treatment of patients with SCD could reduce oxidative damage to RBC, PMN and platelets, thereby alleviating symptoms associated with their pathology. The flow cytometry techniques presented herein could assist in monitoring the efficacy of such treatment.

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  • Authors+Show Affiliations

    ,

    Hadassah - Hebrew University Medical Centre, Jerusalem.

    , , , ,

    Source

    British journal of haematology 132:1 2006 Jan pg 108-13

    MeSH

    Adolescent
    Adult
    Anemia, Sickle Cell
    Antioxidants
    Blood Platelets
    Cell Separation
    Cells, Cultured
    Child
    Child, Preschool
    Erythrocytes
    Female
    Flow Cytometry
    Glutathione
    Humans
    Male
    Neutrophils
    Oxidative Stress
    Reactive Oxygen Species

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    16371026

    Citation

    Amer, Johnny, et al. "Red Blood Cells, Platelets and Polymorphonuclear Neutrophils of Patients With Sickle Cell Disease Exhibit Oxidative Stress That Can Be Ameliorated By Antioxidants." British Journal of Haematology, vol. 132, no. 1, 2006, pp. 108-13.
    Amer J, Ghoti H, Rachmilewitz E, et al. Red blood cells, platelets and polymorphonuclear neutrophils of patients with sickle cell disease exhibit oxidative stress that can be ameliorated by antioxidants. Br J Haematol. 2006;132(1):108-13.
    Amer, J., Ghoti, H., Rachmilewitz, E., Koren, A., Levin, C., & Fibach, E. (2006). Red blood cells, platelets and polymorphonuclear neutrophils of patients with sickle cell disease exhibit oxidative stress that can be ameliorated by antioxidants. British Journal of Haematology, 132(1), pp. 108-13.
    Amer J, et al. Red Blood Cells, Platelets and Polymorphonuclear Neutrophils of Patients With Sickle Cell Disease Exhibit Oxidative Stress That Can Be Ameliorated By Antioxidants. Br J Haematol. 2006;132(1):108-13. PubMed PMID: 16371026.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Red blood cells, platelets and polymorphonuclear neutrophils of patients with sickle cell disease exhibit oxidative stress that can be ameliorated by antioxidants. AU - Amer,Johnny, AU - Ghoti,Hussam, AU - Rachmilewitz,Eliezer, AU - Koren,Ariel, AU - Levin,Carina, AU - Fibach,Eitan, PY - 2005/12/24/pubmed PY - 2006/1/18/medline PY - 2005/12/24/entrez SP - 108 EP - 13 JF - British journal of haematology JO - Br. J. Haematol. VL - 132 IS - 1 N2 - Sickle cell disease (SCD) is basically a red blood cell (RBC) disorder characterised by sickling and haemolysis, but platelets and polymorphonuclear neutrophils (PMN) are also involved. Oxidative damage may play a role in the pathogenesis of SCD. Using flow cytometry, we measured oxidative-state markers simultaneously in RBC, platelets and PMN obtained from 25 normal donors, nine homozygous (SS) patients and six SS/beta-thalassaemia patients. Reactive oxygen species (ROS) and reduced glutathione (GSH) were measured following staining of blood samples with fluorescence probes and gating on specific subpopulations based on size and granularity. Ten- to 30-fold higher ROS production and 20-50% lower GSH content were found in RBC, platelets and PMN from SCD patients versus those of their normal counterparts. This could in part account for the clinical manifestations, such as haemolysis, a hypercoagulable state, recurrent bacterial infections and vaso-occlusive incidences, in SCD. We further showed that exposure of SCD samples to antioxidants, such as N-acetyl-cysteine, vitamin C and vitamin E, decreased their oxidative stress. These results suggest that antioxidant treatment of patients with SCD could reduce oxidative damage to RBC, PMN and platelets, thereby alleviating symptoms associated with their pathology. The flow cytometry techniques presented herein could assist in monitoring the efficacy of such treatment. SN - 0007-1048 UR - https://www.unboundmedicine.com/medline/citation/16371026/Red_blood_cells_platelets_and_polymorphonuclear_neutrophils_of_patients_with_sickle_cell_disease_exhibit_oxidative_stress_that_can_be_ameliorated_by_antioxidants_ L2 - https://doi.org/10.1111/j.1365-2141.2005.05834.x DB - PRIME DP - Unbound Medicine ER -