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The expression of a Pichia stipitis xylose reductase mutant with higher K(M) for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae.
Biotechnol Bioeng. 2006 Mar 05; 93(4):665-73.BB

Abstract

Xylose fermentation by Saccharomyces cerevisiae requires the introduction of a xylose pathway, either similar to that found in the natural xylose-utilizing yeasts Pichia stipitis and Candida shehatae or similar to the bacterial pathway. The use of NAD(P)H-dependent XR and NAD(+)-dependent XDH from P. stipitis creates a cofactor imbalance resulting in xylitol formation. The effect of replacing the native P. stipitis XR with a mutated XR with increased K(M) for NADPH was investigated for xylose fermentation to ethanol by recombinant S. cerevisiae strains. Enhanced ethanol yields accompanied by decreased xylitol yields were obtained in strains carrying the mutated XR. Flux analysis showed that strains harboring the mutated XR utilized a larger fraction of NADH for xylose reduction. The overproduction of the mutated XR resulted in an ethanol yield of 0.40 g per gram of sugar and a xylose consumption rate of 0.16 g per gram of biomass per hour in chemostat culture (0.06/h) with 10 g/L glucose and 10 g/L xylose as carbon source.

Authors+Show Affiliations

Jeppsson M
Department of Applied Microbiology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden.
Bengtsson O
No affiliation info available
Franke K
No affiliation info available
Lee H
No affiliation info available
Hahn-Hägerdal B
No affiliation info available
Gorwa-Grauslund MF
No affiliation info available

MeSH

Aldehyde ReductaseEthanolFermentationGene ExpressionGenes, FungalMutationNADPPichiaRecombination, GeneticSaccharomyces cerevisiaeXylose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16372361

Citation

Jeppsson, Marie, et al. "The Expression of a Pichia Stipitis Xylose Reductase Mutant With Higher K(M) for NADPH Increases Ethanol Production From Xylose in Recombinant Saccharomyces Cerevisiae." Biotechnology and Bioengineering, vol. 93, no. 4, 2006, pp. 665-73.
Jeppsson M, Bengtsson O, Franke K, et al. The expression of a Pichia stipitis xylose reductase mutant with higher K(M) for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae. Biotechnol Bioeng. 2006;93(4):665-73.
Jeppsson, M., Bengtsson, O., Franke, K., Lee, H., Hahn-Hägerdal, B., & Gorwa-Grauslund, M. F. (2006). The expression of a Pichia stipitis xylose reductase mutant with higher K(M) for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae. Biotechnology and Bioengineering, 93(4), 665-73.
Jeppsson M, et al. The Expression of a Pichia Stipitis Xylose Reductase Mutant With Higher K(M) for NADPH Increases Ethanol Production From Xylose in Recombinant Saccharomyces Cerevisiae. Biotechnol Bioeng. 2006 Mar 5;93(4):665-73. PubMed PMID: 16372361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The expression of a Pichia stipitis xylose reductase mutant with higher K(M) for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae. AU - Jeppsson,Marie, AU - Bengtsson,Oskar, AU - Franke,Katja, AU - Lee,Hung, AU - Hahn-Hägerdal,Bärbel, AU - Gorwa-Grauslund,Marie F, PY - 2005/12/24/pubmed PY - 2006/5/5/medline PY - 2005/12/24/entrez SP - 665 EP - 73 JF - Biotechnology and bioengineering JO - Biotechnol Bioeng VL - 93 IS - 4 N2 - Xylose fermentation by Saccharomyces cerevisiae requires the introduction of a xylose pathway, either similar to that found in the natural xylose-utilizing yeasts Pichia stipitis and Candida shehatae or similar to the bacterial pathway. The use of NAD(P)H-dependent XR and NAD(+)-dependent XDH from P. stipitis creates a cofactor imbalance resulting in xylitol formation. The effect of replacing the native P. stipitis XR with a mutated XR with increased K(M) for NADPH was investigated for xylose fermentation to ethanol by recombinant S. cerevisiae strains. Enhanced ethanol yields accompanied by decreased xylitol yields were obtained in strains carrying the mutated XR. Flux analysis showed that strains harboring the mutated XR utilized a larger fraction of NADH for xylose reduction. The overproduction of the mutated XR resulted in an ethanol yield of 0.40 g per gram of sugar and a xylose consumption rate of 0.16 g per gram of biomass per hour in chemostat culture (0.06/h) with 10 g/L glucose and 10 g/L xylose as carbon source. SN - 0006-3592 UR - https://www.unboundmedicine.com/medline/citation/16372361/The_expression_of_a_Pichia_stipitis_xylose_reductase_mutant_with_higher_K_M__for_NADPH_increases_ethanol_production_from_xylose_in_recombinant_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -
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