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Expression of apoptosis-related proteins in peritoneal, ovarian and colorectal endometriosis.
J Reprod Immunol. 2006 Jun; 70(1-2):151-62.JR

Abstract

Endometriosis is defined as the presence of endometrial glands and stroma outside the uterus. Apoptosis, a physiological process by which multicellular organisms eliminate superfluous cells, is altered in tumor tissue. Here we studied the expression of the apoptosis-related proteins p53, bcl-2, bax, p21 and fas in proliferative (n=9) and secretory (n=9) endometrium, and in peritoneal (n=11), ovarian (n=20) and colorectal (n=20) endometriosis, by qualitative and semi-quantitative immunohistochemical methods using the percentage of positive cells and HSCORE analysis. In endometrium, p53, p21 and fas expression was low, whereas bax and bcl-2 expression was elevated. Using HSCORE analysis, only bcl-2 expression varied during the menstrual cycle (48.9+/-34.2% in the proliferative phase, 11.5+/-24.7% in the secretory phase, p=0.01). Using HSCORE analysis, p53 expression was higher in ovarian endometriosis than in peritoneal (p<0.0001) and colorectal endometriosis (p=0.03). P21 expression was higher in ovarian endometriosis than in peritoneal (p=0.01) and colorectal endometriosis (p=0.01). Bcl-2 expression was lower in ovarian endometriosis than in peritoneal (p=0.0002) and colorectal endometriosis (p<0.0001). Fas expression was higher in peritoneal endometriosis than in ovarian (p=0.02) and colorectal endometriosis (p=0.008). In conclusion, these results confirm the involvement of apoptosis in the pathogenesis of endometriosis. Moreover, expression of apoptosis-related proteins varies according to the location of endometriosis suggesting the involvement of different apoptotic pathways.

Authors+Show Affiliations

Service d'Anatomie Pathologie, Hôpital Tenon, AP-HP, UFR Saint Antoine, Paris VI, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16378643

Citation

Dufournet, Charlotte, et al. "Expression of Apoptosis-related Proteins in Peritoneal, Ovarian and Colorectal Endometriosis." Journal of Reproductive Immunology, vol. 70, no. 1-2, 2006, pp. 151-62.
Dufournet C, Uzan C, Fauvet R, et al. Expression of apoptosis-related proteins in peritoneal, ovarian and colorectal endometriosis. J Reprod Immunol. 2006;70(1-2):151-62.
Dufournet, C., Uzan, C., Fauvet, R., Cortez, A., Siffroi, J. P., & Daraï, E. (2006). Expression of apoptosis-related proteins in peritoneal, ovarian and colorectal endometriosis. Journal of Reproductive Immunology, 70(1-2), 151-62.
Dufournet C, et al. Expression of Apoptosis-related Proteins in Peritoneal, Ovarian and Colorectal Endometriosis. J Reprod Immunol. 2006;70(1-2):151-62. PubMed PMID: 16378643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of apoptosis-related proteins in peritoneal, ovarian and colorectal endometriosis. AU - Dufournet,Charlotte, AU - Uzan,Catherine, AU - Fauvet,Raffaèle, AU - Cortez,Annie, AU - Siffroi,Jean-Pierre, AU - Daraï,Emile, Y1 - 2005/12/27/ PY - 2005/08/29/received PY - 2005/08/30/revised PY - 2005/11/18/accepted PY - 2005/12/28/pubmed PY - 2006/8/4/medline PY - 2005/12/28/entrez SP - 151 EP - 62 JF - Journal of reproductive immunology JO - J. Reprod. Immunol. VL - 70 IS - 1-2 N2 - Endometriosis is defined as the presence of endometrial glands and stroma outside the uterus. Apoptosis, a physiological process by which multicellular organisms eliminate superfluous cells, is altered in tumor tissue. Here we studied the expression of the apoptosis-related proteins p53, bcl-2, bax, p21 and fas in proliferative (n=9) and secretory (n=9) endometrium, and in peritoneal (n=11), ovarian (n=20) and colorectal (n=20) endometriosis, by qualitative and semi-quantitative immunohistochemical methods using the percentage of positive cells and HSCORE analysis. In endometrium, p53, p21 and fas expression was low, whereas bax and bcl-2 expression was elevated. Using HSCORE analysis, only bcl-2 expression varied during the menstrual cycle (48.9+/-34.2% in the proliferative phase, 11.5+/-24.7% in the secretory phase, p=0.01). Using HSCORE analysis, p53 expression was higher in ovarian endometriosis than in peritoneal (p<0.0001) and colorectal endometriosis (p=0.03). P21 expression was higher in ovarian endometriosis than in peritoneal (p=0.01) and colorectal endometriosis (p=0.01). Bcl-2 expression was lower in ovarian endometriosis than in peritoneal (p=0.0002) and colorectal endometriosis (p<0.0001). Fas expression was higher in peritoneal endometriosis than in ovarian (p=0.02) and colorectal endometriosis (p=0.008). In conclusion, these results confirm the involvement of apoptosis in the pathogenesis of endometriosis. Moreover, expression of apoptosis-related proteins varies according to the location of endometriosis suggesting the involvement of different apoptotic pathways. SN - 0165-0378 UR - https://www.unboundmedicine.com/medline/citation/16378643/Expression_of_apoptosis_related_proteins_in_peritoneal_ovarian_and_colorectal_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-0378(05)00169-5 DB - PRIME DP - Unbound Medicine ER -