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Identification of anhydroecgonine ethyl ester in the urine of a drug overdose victim.
J Forensic Sci. 2005 Nov; 50(6):1481-5.JF

Abstract

Toxicological evaluation of postmortem urine collected from a 41-year-old deceased white male detected anhydroecgonine ethyl ester (ethylecgonidine, AEEE), a transesterification product of smoked cocaine co-abused with ethanol. A solid phase extraction (SPE) method was used to extract cocaine, AEEE, and related metabolites from urine. SPE on a 1 mL urine sample from the decedent followed by GC-MS detected AEEE. Other metabolites identified by GC-MS included cocaine, cocaethylene, and anhydroecgonine methyl ester (AEME). To determine whether some or all of the AEEE was artifactually produced in the heated GC injector port, an alternative LC-MS method was developed. LC/MS following SPE found at least 50 ng/mL of AEEE in the extract. The mass fragmentation (MS/MS and MS3) of AEEE detected in the urine was compared to spectra of authentic, synthesized compound. AEEE is a potential additional forensic marker for the co-abuse of smoked cocaine and ethanol.

Authors+Show Affiliations

Department of Basic Pharmaceutical Sciences, School of Pharmacy, West Virginia University, Morgantown, WV 26506, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16382849

Citation

Myers, Alan L., et al. "Identification of Anhydroecgonine Ethyl Ester in the Urine of a Drug Overdose Victim." Journal of Forensic Sciences, vol. 50, no. 6, 2005, pp. 1481-5.
Myers AL, Williams HE, Kraner JC, et al. Identification of anhydroecgonine ethyl ester in the urine of a drug overdose victim. J Forensic Sci. 2005;50(6):1481-5.
Myers, A. L., Williams, H. E., Kraner, J. C., & Callery, P. S. (2005). Identification of anhydroecgonine ethyl ester in the urine of a drug overdose victim. Journal of Forensic Sciences, 50(6), 1481-5.
Myers AL, et al. Identification of Anhydroecgonine Ethyl Ester in the Urine of a Drug Overdose Victim. J Forensic Sci. 2005;50(6):1481-5. PubMed PMID: 16382849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of anhydroecgonine ethyl ester in the urine of a drug overdose victim. AU - Myers,Alan L, AU - Williams,Heather E, AU - Kraner,James C, AU - Callery,Patrick S, PY - 2005/12/31/pubmed PY - 2006/2/8/medline PY - 2005/12/31/entrez SP - 1481 EP - 5 JF - Journal of forensic sciences JO - J Forensic Sci VL - 50 IS - 6 N2 - Toxicological evaluation of postmortem urine collected from a 41-year-old deceased white male detected anhydroecgonine ethyl ester (ethylecgonidine, AEEE), a transesterification product of smoked cocaine co-abused with ethanol. A solid phase extraction (SPE) method was used to extract cocaine, AEEE, and related metabolites from urine. SPE on a 1 mL urine sample from the decedent followed by GC-MS detected AEEE. Other metabolites identified by GC-MS included cocaine, cocaethylene, and anhydroecgonine methyl ester (AEME). To determine whether some or all of the AEEE was artifactually produced in the heated GC injector port, an alternative LC-MS method was developed. LC/MS following SPE found at least 50 ng/mL of AEEE in the extract. The mass fragmentation (MS/MS and MS3) of AEEE detected in the urine was compared to spectra of authentic, synthesized compound. AEEE is a potential additional forensic marker for the co-abuse of smoked cocaine and ethanol. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/16382849/Identification_of_anhydroecgonine_ethyl_ester_in_the_urine_of_a_drug_overdose_victim_ DB - PRIME DP - Unbound Medicine ER -