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Dorzolamide and timolol saves retinal ganglion cells in glaucomatous adult rats.
J Ocul Pharmacol Ther. 2005 Dec; 21(6):454-62.JO

Abstract

PURPOSE

This study was designed to evaluate the effects of a dorzolamide-timolol combination or dorzolamide on retinal ganglion cell (RGC) density and intraocular pressure (IOP) in glaucomatous eyes of adult rats.

METHODS

Glaucoma was induced in the right eye of adult Wistar rats by episcleral venous occlusion. One experimental group was administered dorzolamide 2%-timolol 0.5% combination eye drops, while the other experimental group was administered dorzolamide 2% eye drops. Control groups had surgery without drug administration. Drug application was initiated either 2 weeks before surgery (Group A), from the day of surgery (Group B), 2 weeks after surgery (Group C), or 4 weeks after surgery (Group D). RGCs were labeled by intratectal Fluorogold injections and counted from flat-mount preparations, and IOP was measured using Tonopen.

RESULTS

Both dorzolamide-timolol combination and dorzolamide, when applied topically, significantly reduced IOP and improved RGC densities in experimental eyes when compared to control eyes. Earlier initiation, as well as longer duration of drug application, resulted in higher RGC densities.

CONCLUSIONS

Topical application of a dorzolamide-timolol combination or dorzolamide saved RGCs to a significant extent and reduced IOP in glaucomatous rat eyes.

Authors+Show Affiliations

Department of Ophthalmology & Cell Biology and Anatomy, New York Medical College, Valhalla, NY 10595, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16386087

Citation

Sarup, Vimal, et al. "Dorzolamide and Timolol Saves Retinal Ganglion Cells in Glaucomatous Adult Rats." Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics, vol. 21, no. 6, 2005, pp. 454-62.
Sarup V, McEwan GC, Thompson C, et al. Dorzolamide and timolol saves retinal ganglion cells in glaucomatous adult rats. J Ocul Pharmacol Ther. 2005;21(6):454-62.
Sarup, V., McEwan, G. C., Thompson, C., Patil, K. A., & Sharma, S. C. (2005). Dorzolamide and timolol saves retinal ganglion cells in glaucomatous adult rats. Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics, 21(6), 454-62.
Sarup V, et al. Dorzolamide and Timolol Saves Retinal Ganglion Cells in Glaucomatous Adult Rats. J Ocul Pharmacol Ther. 2005;21(6):454-62. PubMed PMID: 16386087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dorzolamide and timolol saves retinal ganglion cells in glaucomatous adult rats. AU - Sarup,Vimal, AU - McEwan,Gavin C, AU - Thompson,Carl, AU - Patil,Kiran A, AU - Sharma,Sansar C, PY - 2006/1/3/pubmed PY - 2006/2/4/medline PY - 2006/1/3/entrez SP - 454 EP - 62 JF - Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics JO - J Ocul Pharmacol Ther VL - 21 IS - 6 N2 - PURPOSE: This study was designed to evaluate the effects of a dorzolamide-timolol combination or dorzolamide on retinal ganglion cell (RGC) density and intraocular pressure (IOP) in glaucomatous eyes of adult rats. METHODS: Glaucoma was induced in the right eye of adult Wistar rats by episcleral venous occlusion. One experimental group was administered dorzolamide 2%-timolol 0.5% combination eye drops, while the other experimental group was administered dorzolamide 2% eye drops. Control groups had surgery without drug administration. Drug application was initiated either 2 weeks before surgery (Group A), from the day of surgery (Group B), 2 weeks after surgery (Group C), or 4 weeks after surgery (Group D). RGCs were labeled by intratectal Fluorogold injections and counted from flat-mount preparations, and IOP was measured using Tonopen. RESULTS: Both dorzolamide-timolol combination and dorzolamide, when applied topically, significantly reduced IOP and improved RGC densities in experimental eyes when compared to control eyes. Earlier initiation, as well as longer duration of drug application, resulted in higher RGC densities. CONCLUSIONS: Topical application of a dorzolamide-timolol combination or dorzolamide saved RGCs to a significant extent and reduced IOP in glaucomatous rat eyes. SN - 1080-7683 UR - https://www.unboundmedicine.com/medline/citation/16386087/Dorzolamide_and_timolol_saves_retinal_ganglion_cells_in_glaucomatous_adult_rats_ L2 - https://www.liebertpub.com/doi/10.1089/jop.2005.21.454?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -