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Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration.
Matrix Biol. 2006 Apr; 25(3):158-65.MB

Abstract

The ability to generate tissue-specific ablation of gene expression has been extremely useful in connective tissue biology, as it can potentially overcome the early embryonic lethal phenotype often associated with universal gene knockout. The value of tissue-specific knockouts can be enhanced by also allowing gene ablation to occur at specific times during development, growth or aging. In the present work a transgenic mouse has been generated in which expression of Cre recombinase is under control of both the type II collagen promoter to allow cartilage-specific expression and a doxycycline response element to permit temporal control of expression. This mouse has been crossed with the Rosa26R reporter mouse, which possesses a floxed repressor element associated with a lacZ transgene, in order to validate the functional efficacy of the conditionally expressed Cre. The results demonstrate that excision of the floxed element can be achieved specifically in cartilage at different times during embryonic and juvenile development. The conditional Cre transgenic mouse should be a valuable tool to all interested in skeletal development.

Authors+Show Affiliations

Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada, H3G 1A6.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16386413

Citation

Grover, Judy, and Peter J. Roughley. "Generation of a Transgenic Mouse in Which Cre Recombinase Is Expressed Under Control of the Type II Collagen Promoter and Doxycycline Administration." Matrix Biology : Journal of the International Society for Matrix Biology, vol. 25, no. 3, 2006, pp. 158-65.
Grover J, Roughley PJ. Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration. Matrix Biol. 2006;25(3):158-65.
Grover, J., & Roughley, P. J. (2006). Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration. Matrix Biology : Journal of the International Society for Matrix Biology, 25(3), 158-65.
Grover J, Roughley PJ. Generation of a Transgenic Mouse in Which Cre Recombinase Is Expressed Under Control of the Type II Collagen Promoter and Doxycycline Administration. Matrix Biol. 2006;25(3):158-65. PubMed PMID: 16386413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration. AU - Grover,Judy, AU - Roughley,Peter J, Y1 - 2005/12/28/ PY - 2005/08/24/received PY - 2005/11/07/revised PY - 2005/11/09/accepted PY - 2006/1/3/pubmed PY - 2006/11/1/medline PY - 2006/1/3/entrez SP - 158 EP - 65 JF - Matrix biology : journal of the International Society for Matrix Biology JO - Matrix Biol VL - 25 IS - 3 N2 - The ability to generate tissue-specific ablation of gene expression has been extremely useful in connective tissue biology, as it can potentially overcome the early embryonic lethal phenotype often associated with universal gene knockout. The value of tissue-specific knockouts can be enhanced by also allowing gene ablation to occur at specific times during development, growth or aging. In the present work a transgenic mouse has been generated in which expression of Cre recombinase is under control of both the type II collagen promoter to allow cartilage-specific expression and a doxycycline response element to permit temporal control of expression. This mouse has been crossed with the Rosa26R reporter mouse, which possesses a floxed repressor element associated with a lacZ transgene, in order to validate the functional efficacy of the conditionally expressed Cre. The results demonstrate that excision of the floxed element can be achieved specifically in cartilage at different times during embryonic and juvenile development. The conditional Cre transgenic mouse should be a valuable tool to all interested in skeletal development. SN - 0945-053X UR - https://www.unboundmedicine.com/medline/citation/16386413/Generation_of_a_transgenic_mouse_in_which_Cre_recombinase_is_expressed_under_control_of_the_type_II_collagen_promoter_and_doxycycline_administration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0945-053X(05)00151-4 DB - PRIME DP - Unbound Medicine ER -