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Sample stacking for the analysis of penicillins by microemulsion electrokinetic capillary chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 02; 831(1-2):196-204.JC

Abstract

We present a method for determining eight penicillin antibiotics using microemulsion electrokinetic chromatography (MEEKC). We studied how the composition of the microemulsion affected separation by modifying such parameters as the surfactant or the addition of organic solvents. The best microemulsion system consisted of 0.5% ethyl acetate, 1.2% 1-butanol, 2% Brij 35, 10% 2-butanol and 86.3% 10 mM borate buffer at pH 10. We studied the suitability of this microemulsion composition for analyzing a commercial drug. To improve the sensitivity of the method, we used the stacking technique reversed electrode polarity stacking mode (REPSM), which increased the detection limits by about 40-fold.

Authors+Show Affiliations

Puig P
Department of Analytical Chemistry and Organic Chemistry, Rovira i Virgili University, Marcel lí Domingo s/n, E-43007 Tarragona, Spain.
Borrull F
No affiliation info available
Aguilar C
No affiliation info available
Calull M
No affiliation info available

MeSH

BuffersChromatography, Micellar Electrokinetic CapillaryEmulsionsHydrogen-Ion ConcentrationPenicillinsSensitivity and SpecificitySolventsSurface-Active Agents

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

16386475

Citation

Puig, Patricia, et al. "Sample Stacking for the Analysis of Penicillins By Microemulsion Electrokinetic Capillary Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 831, no. 1-2, 2006, pp. 196-204.
Puig P, Borrull F, Aguilar C, et al. Sample stacking for the analysis of penicillins by microemulsion electrokinetic capillary chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;831(1-2):196-204.
Puig, P., Borrull, F., Aguilar, C., & Calull, M. (2006). Sample stacking for the analysis of penicillins by microemulsion electrokinetic capillary chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 831(1-2), 196-204.
Puig P, et al. Sample Stacking for the Analysis of Penicillins By Microemulsion Electrokinetic Capillary Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 2;831(1-2):196-204. PubMed PMID: 16386475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sample stacking for the analysis of penicillins by microemulsion electrokinetic capillary chromatography. AU - Puig,Patricia, AU - Borrull,Francesc, AU - Aguilar,Carme, AU - Calull,Marta, Y1 - 2005/12/28/ PY - 2005/02/03/received PY - 2005/11/30/revised PY - 2005/12/05/accepted PY - 2006/1/3/pubmed PY - 2006/4/4/medline PY - 2006/1/3/entrez SP - 196 EP - 204 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 831 IS - 1-2 N2 - We present a method for determining eight penicillin antibiotics using microemulsion electrokinetic chromatography (MEEKC). We studied how the composition of the microemulsion affected separation by modifying such parameters as the surfactant or the addition of organic solvents. The best microemulsion system consisted of 0.5% ethyl acetate, 1.2% 1-butanol, 2% Brij 35, 10% 2-butanol and 86.3% 10 mM borate buffer at pH 10. We studied the suitability of this microemulsion composition for analyzing a commercial drug. To improve the sensitivity of the method, we used the stacking technique reversed electrode polarity stacking mode (REPSM), which increased the detection limits by about 40-fold. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16386475/Sample_stacking_for_the_analysis_of_penicillins_by_microemulsion_electrokinetic_capillary_chromatography_ DB - PRIME DP - Unbound Medicine ER -