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Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2.
Toxicol Appl Pharmacol. 2006 Jul 01; 214(1):8-15.TA

Abstract

Berberine, a compound isolated from medicinal herbs, has been reported with many pharmacological effects related to anti-cancer and anti-inflammation capabilities. In this study, we observed that berberine exerted a dose- and time-dependent inhibitory effect on the motility and invasion ability of a highly metastatic A549 cells under non-cytotoxic concentrations. In cancer cell migration and invasion process, matrix-degrading proteinases are required. A549 cell treated with berberine at various concentrations showed reduced ECM proteinases including matrix metalloproteinase-2 (MMP2) and urokinase-plasminogen activator (u-PA) by gelatin and casein zymography analysis. The inhibitory effect is likely to be at the transcriptional level, since the reduction in the transcripts levels was corresponding to the proteins. Moreover, berberine also exerted its action via regulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and urokinase-plasminogen activator inhibitor (PAI). The upstream mediators of the effect involved c-jun, c-fos and NF-kappaB, as evidenced by reduced phosphorylation of the proteins. These findings suggest that berberine possesses an anti-metastatic effect in non-small lung cancer cell and may, therefore, be helpful in clinical treatment.

Authors+Show Affiliations

Institute of Biochemistry and Biotechnology, College of Medicine, Chung Shan Medical University, No 110, section 2, Chien Kauo N. Road, Taichung 402, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16387334

Citation

Peng, Pei-Ling, et al. "Inhibitory Effect of Berberine On the Invasion of Human Lung Cancer Cells Via Decreased Productions of Urokinase-plasminogen Activator and Matrix Metalloproteinase-2." Toxicology and Applied Pharmacology, vol. 214, no. 1, 2006, pp. 8-15.
Peng PL, Hsieh YS, Wang CJ, et al. Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. Toxicol Appl Pharmacol. 2006;214(1):8-15.
Peng, P. L., Hsieh, Y. S., Wang, C. J., Hsu, J. L., & Chou, F. P. (2006). Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. Toxicology and Applied Pharmacology, 214(1), 8-15.
Peng PL, et al. Inhibitory Effect of Berberine On the Invasion of Human Lung Cancer Cells Via Decreased Productions of Urokinase-plasminogen Activator and Matrix Metalloproteinase-2. Toxicol Appl Pharmacol. 2006 Jul 1;214(1):8-15. PubMed PMID: 16387334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. AU - Peng,Pei-Ling, AU - Hsieh,Yih-Shou, AU - Wang,Chau-Jong, AU - Hsu,Jui-Ling, AU - Chou,Fen-Pi, Y1 - 2006/01/04/ PY - 2005/07/25/received PY - 2005/10/26/revised PY - 2005/11/19/accepted PY - 2006/1/3/pubmed PY - 2006/8/12/medline PY - 2006/1/3/entrez SP - 8 EP - 15 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 214 IS - 1 N2 - Berberine, a compound isolated from medicinal herbs, has been reported with many pharmacological effects related to anti-cancer and anti-inflammation capabilities. In this study, we observed that berberine exerted a dose- and time-dependent inhibitory effect on the motility and invasion ability of a highly metastatic A549 cells under non-cytotoxic concentrations. In cancer cell migration and invasion process, matrix-degrading proteinases are required. A549 cell treated with berberine at various concentrations showed reduced ECM proteinases including matrix metalloproteinase-2 (MMP2) and urokinase-plasminogen activator (u-PA) by gelatin and casein zymography analysis. The inhibitory effect is likely to be at the transcriptional level, since the reduction in the transcripts levels was corresponding to the proteins. Moreover, berberine also exerted its action via regulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and urokinase-plasminogen activator inhibitor (PAI). The upstream mediators of the effect involved c-jun, c-fos and NF-kappaB, as evidenced by reduced phosphorylation of the proteins. These findings suggest that berberine possesses an anti-metastatic effect in non-small lung cancer cell and may, therefore, be helpful in clinical treatment. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/16387334/Inhibitory_effect_of_berberine_on_the_invasion_of_human_lung_cancer_cells_via_decreased_productions_of_urokinase_plasminogen_activator_and_matrix_metalloproteinase_2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(05)00654-X DB - PRIME DP - Unbound Medicine ER -