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Human T lymphotropic virus types I and II western blot seroindeterminate status and its association with exposure to prototype HTLV-I.
J Infect Dis. 2006 Feb 01; 193(3):427-37.JI

Abstract

Human T lymphotropic virus types I and II (HTLV-I/II) Western blot (WB) seroindeterminate status, which is defined as an incomplete banding pattern of HTLV protein Gag (p19 or p24) or Env (GD21 or rgp46), is commonly observed. To investigate the significance of this finding, we examined HTLV-I/II serostatus and HTLV-I proviral load in 2 groups of individuals with WB seroindeterminate status. Low proviral loads were detected in 42% of patients with neurologic symptoms and 44% of voluntary blood donors. These data suggest that a subset of WB seroindeterminate individuals may be infected with prototype HTLV-I. To confirm this hypothesis, we evaluated HTLV-I/II serostatus and proviral load in prospectively collected specimens from 66 WB seronegative patients who had received HTLV-I-infected blood products by transfusion. Eight individuals developed WB seroindeterminate profiles after the transfusion. In addition, using a human leukocyte antigen type A*201-restricted HTLV-I Tax11-19 tetramer, we detected virus-specific CD8(+) T cells in peripheral blood mononuclear cells from WB seroindeterminate patients. These CD8(+) T cells were effective at targeting HTLV-I-infected cells. Collectively, the results suggest that HTLV-I/II WB seroindeterminate status may reflect a history of HTLV-I exposure. Our findings warrant further investigation of the possible clinical outcomes associated with WB seroindeterminate status.

Authors+Show Affiliations

Viral Immunology Section, Neuroimmunology Branch, National Institute of Neurologic Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16388491

Citation

Yao, Karen, et al. "Human T Lymphotropic Virus Types I and II Western Blot Seroindeterminate Status and Its Association With Exposure to Prototype HTLV-I." The Journal of Infectious Diseases, vol. 193, no. 3, 2006, pp. 427-37.
Yao K, Hisada M, Maloney E, et al. Human T lymphotropic virus types I and II western blot seroindeterminate status and its association with exposure to prototype HTLV-I. J Infect Dis. 2006;193(3):427-37.
Yao, K., Hisada, M., Maloney, E., Yamano, Y., Hanchard, B., Wilks, R., Rios, M., & Jacobson, S. (2006). Human T lymphotropic virus types I and II western blot seroindeterminate status and its association with exposure to prototype HTLV-I. The Journal of Infectious Diseases, 193(3), 427-37.
Yao K, et al. Human T Lymphotropic Virus Types I and II Western Blot Seroindeterminate Status and Its Association With Exposure to Prototype HTLV-I. J Infect Dis. 2006 Feb 1;193(3):427-37. PubMed PMID: 16388491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human T lymphotropic virus types I and II western blot seroindeterminate status and its association with exposure to prototype HTLV-I. AU - Yao,Karen, AU - Hisada,Michie, AU - Maloney,Elizabeth, AU - Yamano,Yoshihisa, AU - Hanchard,Barrie, AU - Wilks,Rainford, AU - Rios,Maria, AU - Jacobson,Steven, Y1 - 2005/12/28/ PY - 2005/04/05/received PY - 2005/07/06/accepted PY - 2006/1/3/pubmed PY - 2006/3/31/medline PY - 2006/1/3/entrez SP - 427 EP - 37 JF - The Journal of infectious diseases JO - J Infect Dis VL - 193 IS - 3 N2 - Human T lymphotropic virus types I and II (HTLV-I/II) Western blot (WB) seroindeterminate status, which is defined as an incomplete banding pattern of HTLV protein Gag (p19 or p24) or Env (GD21 or rgp46), is commonly observed. To investigate the significance of this finding, we examined HTLV-I/II serostatus and HTLV-I proviral load in 2 groups of individuals with WB seroindeterminate status. Low proviral loads were detected in 42% of patients with neurologic symptoms and 44% of voluntary blood donors. These data suggest that a subset of WB seroindeterminate individuals may be infected with prototype HTLV-I. To confirm this hypothesis, we evaluated HTLV-I/II serostatus and proviral load in prospectively collected specimens from 66 WB seronegative patients who had received HTLV-I-infected blood products by transfusion. Eight individuals developed WB seroindeterminate profiles after the transfusion. In addition, using a human leukocyte antigen type A*201-restricted HTLV-I Tax11-19 tetramer, we detected virus-specific CD8(+) T cells in peripheral blood mononuclear cells from WB seroindeterminate patients. These CD8(+) T cells were effective at targeting HTLV-I-infected cells. Collectively, the results suggest that HTLV-I/II WB seroindeterminate status may reflect a history of HTLV-I exposure. Our findings warrant further investigation of the possible clinical outcomes associated with WB seroindeterminate status. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/16388491/Human_T_lymphotropic_virus_types_I_and_II_western_blot_seroindeterminate_status_and_its_association_with_exposure_to_prototype_HTLV_I_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1086/499273 DB - PRIME DP - Unbound Medicine ER -