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N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusion.
Nephrol Dial Transplant 2006; 21(5):1240-7ND

Abstract

BACKGROUND

The aim of the present study is to examine the effects of N-acetylcysteine (NAC), a thiol-containing anti-oxidant, on renal function and morphology, and biomarkers of oxidative stress, in rats subjected to renal ischaemia-reperfusion (IR).

METHODS

Sprague-Dawley rats underwent unilateral nephrectomy and either contralateral renal IR (40 min of renal arterial clamping), or sham manipulation. Treatment groups were: (1) IR-Saline, (2) IR-NAC, (3) Sham-Saline and (4) Sham-NAC. The N-acetylcysteine was administered in a dose of 200 mg/kg intraperitoneally at 24, 12 and 2 h before, and 24, 48 and 72 h after, renal IR. Plasma creatinine was measured on days 1, 3 and 7 after IR, and kidney histology was assessed on day 7. In separate groups of animals we measured renal levels of the anti-oxidant glutathione, markers of systemic oxidative stress (plasma ascorbyl radical, urinary 8-iso-prostaglandin F2alpha), and glomerular filtration rate (GFR) by 51Cr-EDTA clearance, on day 1 after renal IR.

RESULTS

Treatment with NAC ameliorated the decline in GFR and reduced hyperkalaemia on day 1 (P<0.05), lowered plasma creatinine levels on days 1 and 3 (P<0.05), and decreased renal interstitial inflammation on day 7 (P<0.05), after renal IR. Kidney glutathione levels decreased significantly in group IR-Saline in response to IR (P<0.05), but were completely repleted in group IR-NAC. Groups with renal IR injury and acute renal failure showed increased plasma ascorbyl radical levels, and elevated urinary 8-iso-prostaglandin F2alpha excretion, compared with sham (P<0.05). N-acetylcysteine treatment reduced plasma ascorbyl concentrations 24 h after renal IR (P<0.05), but had no effect on the rate of urinary 8-iso-prostaglandin F2alpha excretion.

CONCLUSIONS

N-acetylcysteine improves kidney function, and reduces renal interstitial inflammation, in rats subjected to renal IR. These effects were associated with increased renal glutathione levels, and decreased plasma ascorbyl concentrations, suggesting that NAC attenuates renal and systemic oxidative stress in this model.

Authors+Show Affiliations

Department of Anaesthesiology and Intensive Care, Institute of Surgical Sciences, The Sahlgrenska Academy at Göteborg University, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. nicoletta.nitescu@vgregion.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16390850

Citation

Nitescu, Nicoletta, et al. "N-acetylcysteine Attenuates Kidney Injury in Rats Subjected to Renal Ischaemia-reperfusion." Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association, vol. 21, no. 5, 2006, pp. 1240-7.
Nitescu N, Ricksten SE, Marcussen N, et al. N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusion. Nephrol Dial Transplant. 2006;21(5):1240-7.
Nitescu, N., Ricksten, S. E., Marcussen, N., Haraldsson, B., Nilsson, U., Basu, S., & Guron, G. (2006). N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusion. Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association, 21(5), pp. 1240-7.
Nitescu N, et al. N-acetylcysteine Attenuates Kidney Injury in Rats Subjected to Renal Ischaemia-reperfusion. Nephrol Dial Transplant. 2006;21(5):1240-7. PubMed PMID: 16390850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-acetylcysteine attenuates kidney injury in rats subjected to renal ischaemia-reperfusion. AU - Nitescu,Nicoletta, AU - Ricksten,Sven-Erik, AU - Marcussen,Niels, AU - Haraldsson,Börje, AU - Nilsson,Ulf, AU - Basu,Samar, AU - Guron,Gregor, Y1 - 2006/01/03/ PY - 2006/1/5/pubmed PY - 2006/7/11/medline PY - 2006/1/5/entrez SP - 1240 EP - 7 JF - Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association JO - Nephrol. Dial. Transplant. VL - 21 IS - 5 N2 - BACKGROUND: The aim of the present study is to examine the effects of N-acetylcysteine (NAC), a thiol-containing anti-oxidant, on renal function and morphology, and biomarkers of oxidative stress, in rats subjected to renal ischaemia-reperfusion (IR). METHODS: Sprague-Dawley rats underwent unilateral nephrectomy and either contralateral renal IR (40 min of renal arterial clamping), or sham manipulation. Treatment groups were: (1) IR-Saline, (2) IR-NAC, (3) Sham-Saline and (4) Sham-NAC. The N-acetylcysteine was administered in a dose of 200 mg/kg intraperitoneally at 24, 12 and 2 h before, and 24, 48 and 72 h after, renal IR. Plasma creatinine was measured on days 1, 3 and 7 after IR, and kidney histology was assessed on day 7. In separate groups of animals we measured renal levels of the anti-oxidant glutathione, markers of systemic oxidative stress (plasma ascorbyl radical, urinary 8-iso-prostaglandin F2alpha), and glomerular filtration rate (GFR) by 51Cr-EDTA clearance, on day 1 after renal IR. RESULTS: Treatment with NAC ameliorated the decline in GFR and reduced hyperkalaemia on day 1 (P<0.05), lowered plasma creatinine levels on days 1 and 3 (P<0.05), and decreased renal interstitial inflammation on day 7 (P<0.05), after renal IR. Kidney glutathione levels decreased significantly in group IR-Saline in response to IR (P<0.05), but were completely repleted in group IR-NAC. Groups with renal IR injury and acute renal failure showed increased plasma ascorbyl radical levels, and elevated urinary 8-iso-prostaglandin F2alpha excretion, compared with sham (P<0.05). N-acetylcysteine treatment reduced plasma ascorbyl concentrations 24 h after renal IR (P<0.05), but had no effect on the rate of urinary 8-iso-prostaglandin F2alpha excretion. CONCLUSIONS: N-acetylcysteine improves kidney function, and reduces renal interstitial inflammation, in rats subjected to renal IR. These effects were associated with increased renal glutathione levels, and decreased plasma ascorbyl concentrations, suggesting that NAC attenuates renal and systemic oxidative stress in this model. SN - 0931-0509 UR - https://www.unboundmedicine.com/medline/citation/16390850/N_acetylcysteine_attenuates_kidney_injury_in_rats_subjected_to_renal_ischaemia_reperfusion_ L2 - https://academic.oup.com/ndt/article-lookup/doi/10.1093/ndt/gfk032 DB - PRIME DP - Unbound Medicine ER -