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Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis.
J Cell Biol. 2006 Jan 02; 172(1):103-13.JC

Abstract

We assessed viable Pax7(-/-) mice in 129Sv/J background and observed reduced growth and marked muscle wasting together with a complete absence of functional satellite cells. Acute injury resulted in an extreme deficit in muscle regeneration. However, a small number of regenerated myofibers were detected, suggesting the presence of residual myogenic cells in Pax7-deficient muscle. Rare Pax3(+)/MyoD+ myoblasts were recovered from Pax7(-/-) muscle homogenates and cultures of myofiber bundles but not from single myofibers free of interstitial tissues. Finally, we identified Pax3+ cells in the muscle interstitial environment and demonstrated that they coexpressed MyoD during regeneration. Sublaminar satellite cells in hind limb muscle did not express detectable levels of Pax3 protein or messenger RNA. Therefore, we conclude that interstitial Pax3+ cells represent a novel myogenic population that is distinct from the sublaminar satellite cell lineage and that Pax7 is essential for the formation of functional myogenic progenitors from sublaminar satellite cells.

Authors+Show Affiliations

Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16391000

Citation

Kuang, Shihuan, et al. "Distinct Roles for Pax7 and Pax3 in Adult Regenerative Myogenesis." The Journal of Cell Biology, vol. 172, no. 1, 2006, pp. 103-13.
Kuang S, Chargé SB, Seale P, et al. Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis. J Cell Biol. 2006;172(1):103-13.
Kuang, S., Chargé, S. B., Seale, P., Huh, M., & Rudnicki, M. A. (2006). Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis. The Journal of Cell Biology, 172(1), 103-13.
Kuang S, et al. Distinct Roles for Pax7 and Pax3 in Adult Regenerative Myogenesis. J Cell Biol. 2006 Jan 2;172(1):103-13. PubMed PMID: 16391000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis. AU - Kuang,Shihuan, AU - Chargé,Sophie B, AU - Seale,Patrick, AU - Huh,Michael, AU - Rudnicki,Michael A, PY - 2006/1/5/pubmed PY - 2006/4/14/medline PY - 2006/1/5/entrez SP - 103 EP - 13 JF - The Journal of cell biology JO - J. Cell Biol. VL - 172 IS - 1 N2 - We assessed viable Pax7(-/-) mice in 129Sv/J background and observed reduced growth and marked muscle wasting together with a complete absence of functional satellite cells. Acute injury resulted in an extreme deficit in muscle regeneration. However, a small number of regenerated myofibers were detected, suggesting the presence of residual myogenic cells in Pax7-deficient muscle. Rare Pax3(+)/MyoD+ myoblasts were recovered from Pax7(-/-) muscle homogenates and cultures of myofiber bundles but not from single myofibers free of interstitial tissues. Finally, we identified Pax3+ cells in the muscle interstitial environment and demonstrated that they coexpressed MyoD during regeneration. Sublaminar satellite cells in hind limb muscle did not express detectable levels of Pax3 protein or messenger RNA. Therefore, we conclude that interstitial Pax3+ cells represent a novel myogenic population that is distinct from the sublaminar satellite cell lineage and that Pax7 is essential for the formation of functional myogenic progenitors from sublaminar satellite cells. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/16391000/Distinct_roles_for_Pax7_and_Pax3_in_adult_regenerative_myogenesis_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.200508001 DB - PRIME DP - Unbound Medicine ER -