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Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome.
Genome. 2005 Dec; 48(6):959-70.G

Abstract

Overall, 253 genomic wheat (Triticum aestivum) microsatellite markers were studied for their transferability to the diploid species Aegilops speltoides, Aegilops longissima, and Aegilops searsii, representing the S genome. In total, 88% of all the analyzed primer pairs of markers derived from the B genome of hexaploid wheat amplified DNA fragments in the genomes of the studied species. The transferability of simple sequence repeat (SSR) markers of the T. aestivum A and D genomes totaled 74%. Triticum aestivum-Ae. speltoides, T. aestivum-Ae. longissima, and T. aestivum-Ae. searsii chromosome addition lines allowed us to determine the chromosomal localizations of 103 microsatellite markers in the Aegilops genomes. The majority of them were localized to homoeologous chromosomes in the genome of Aegilops. Several instances of nonhomoeologous localization of T. aestivum SSR markers in the Aegilops genome were considered to be either amplification of other loci or putative translocations. The results of microsatellite analysis were used to study phylogenetic relationships among the 3 species of the Sitopsis section (Ae. speltoides, Ae. longissima, and Ae. searsii) and T. aestivum. The dendrogram obtained generally reflects the current views on phylogenetic relationships among these species.

Authors+Show Affiliations

Adonina IG
Institute of Cytology and Genetics, Novosibirsk, Russia. adonina@bionet.nsc.ru
Salina EA
No affiliation info available
Pestsova EG
No affiliation info available
Röder MS
No affiliation info available

MeSH

Chromosome MappingDiploidyGene Transfer TechniquesGenetic MarkersGenome, PlantMicrosatellite RepeatsPhylogenyPoaceaeTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16391665

Citation

Adonina, I G., et al. "Transferability of Wheat Microsatellites to Diploid Aegilops Species and Determination of Chromosomal Localizations of Microsatellites in the S Genome." Genome, vol. 48, no. 6, 2005, pp. 959-70.
Adonina IG, Salina EA, Pestsova EG, et al. Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome. Genome. 2005;48(6):959-70.
Adonina, I. G., Salina, E. A., Pestsova, E. G., & Röder, M. S. (2005). Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome. Genome, 48(6), 959-70.
Adonina IG, et al. Transferability of Wheat Microsatellites to Diploid Aegilops Species and Determination of Chromosomal Localizations of Microsatellites in the S Genome. Genome. 2005;48(6):959-70. PubMed PMID: 16391665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome. AU - Adonina,I G, AU - Salina,E A, AU - Pestsova,E G, AU - Röder,M S, PY - 2006/1/5/pubmed PY - 2007/3/9/medline PY - 2006/1/5/entrez SP - 959 EP - 70 JF - Genome JO - Genome VL - 48 IS - 6 N2 - Overall, 253 genomic wheat (Triticum aestivum) microsatellite markers were studied for their transferability to the diploid species Aegilops speltoides, Aegilops longissima, and Aegilops searsii, representing the S genome. In total, 88% of all the analyzed primer pairs of markers derived from the B genome of hexaploid wheat amplified DNA fragments in the genomes of the studied species. The transferability of simple sequence repeat (SSR) markers of the T. aestivum A and D genomes totaled 74%. Triticum aestivum-Ae. speltoides, T. aestivum-Ae. longissima, and T. aestivum-Ae. searsii chromosome addition lines allowed us to determine the chromosomal localizations of 103 microsatellite markers in the Aegilops genomes. The majority of them were localized to homoeologous chromosomes in the genome of Aegilops. Several instances of nonhomoeologous localization of T. aestivum SSR markers in the Aegilops genome were considered to be either amplification of other loci or putative translocations. The results of microsatellite analysis were used to study phylogenetic relationships among the 3 species of the Sitopsis section (Ae. speltoides, Ae. longissima, and Ae. searsii) and T. aestivum. The dendrogram obtained generally reflects the current views on phylogenetic relationships among these species. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/16391665/Transferability_of_wheat_microsatellites_to_diploid_Aegilops_species_and_determination_of_chromosomal_localizations_of_microsatellites_in_the_S_genome_ DB - PRIME DP - Unbound Medicine ER -