TY - JOUR T1 - Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids. AU - Kumazawa,T, AU - Sato,K, AU - Seno,H, AU - Suzuki,O, PY - 1992/5/1/pubmed PY - 1992/5/1/medline PY - 1992/5/1/entrez SP - 159 EP - 66 JF - Forensic science international JO - Forensic Sci Int VL - 54 IS - 2 N2 - A simple and rapid method, for the isolation of eight triazine herbicides from human serum and urine, using Sep-Pak C18 cartridges is presented. After mixing with distilled water, serum and urine samples containing the herbicides, were loaded on Sep-Pak C18 cartridges and eluted with either chloroform only or chloroform/methanol (9:1). The herbicides were detected by capillary gas chromatography with both flame ionization detection (FID) and nitrogen-phosphorus detection (NPD). Separation of eight triazine herbicides from each other and from impurities was generally satisfactory with the use of a non-polar DB-1 capillary column. Recovery of most compounds was excellent for both chloroform and chloroform/methanol (9:1) as elution solvents. Backgrounds were cleaner and evaporation time was shorter for the chloroform only than for the chloroform/methanol (9:1). The NPD gave sensitivity more than 10-20 times higher than that of FID. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/1639282/Rapid_isolation_with_Sep_Pak_C18_cartridges_and_capillary_gas_chromatography_of_triazine_herbicides_in_human_body_fluids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0379-0738(92)90160-X DB - PRIME DP - Unbound Medicine ER -