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Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids.
Forensic Sci Int. 1992 May; 54(2):159-66.FS

Abstract

A simple and rapid method, for the isolation of eight triazine herbicides from human serum and urine, using Sep-Pak C18 cartridges is presented. After mixing with distilled water, serum and urine samples containing the herbicides, were loaded on Sep-Pak C18 cartridges and eluted with either chloroform only or chloroform/methanol (9:1). The herbicides were detected by capillary gas chromatography with both flame ionization detection (FID) and nitrogen-phosphorus detection (NPD). Separation of eight triazine herbicides from each other and from impurities was generally satisfactory with the use of a non-polar DB-1 capillary column. Recovery of most compounds was excellent for both chloroform and chloroform/methanol (9:1) as elution solvents. Backgrounds were cleaner and evaporation time was shorter for the chloroform only than for the chloroform/methanol (9:1). The NPD gave sensitivity more than 10-20 times higher than that of FID.

Authors+Show Affiliations

Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1639282

Citation

Kumazawa, T, et al. "Rapid Isolation With Sep-Pak C18 Cartridges and Capillary Gas Chromatography of Triazine Herbicides in Human Body Fluids." Forensic Science International, vol. 54, no. 2, 1992, pp. 159-66.
Kumazawa T, Sato K, Seno H, et al. Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids. Forensic Sci Int. 1992;54(2):159-66.
Kumazawa, T., Sato, K., Seno, H., & Suzuki, O. (1992). Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids. Forensic Science International, 54(2), 159-66.
Kumazawa T, et al. Rapid Isolation With Sep-Pak C18 Cartridges and Capillary Gas Chromatography of Triazine Herbicides in Human Body Fluids. Forensic Sci Int. 1992;54(2):159-66. PubMed PMID: 1639282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids. AU - Kumazawa,T, AU - Sato,K, AU - Seno,H, AU - Suzuki,O, PY - 1992/5/1/pubmed PY - 1992/5/1/medline PY - 1992/5/1/entrez SP - 159 EP - 66 JF - Forensic science international JO - Forensic Sci Int VL - 54 IS - 2 N2 - A simple and rapid method, for the isolation of eight triazine herbicides from human serum and urine, using Sep-Pak C18 cartridges is presented. After mixing with distilled water, serum and urine samples containing the herbicides, were loaded on Sep-Pak C18 cartridges and eluted with either chloroform only or chloroform/methanol (9:1). The herbicides were detected by capillary gas chromatography with both flame ionization detection (FID) and nitrogen-phosphorus detection (NPD). Separation of eight triazine herbicides from each other and from impurities was generally satisfactory with the use of a non-polar DB-1 capillary column. Recovery of most compounds was excellent for both chloroform and chloroform/methanol (9:1) as elution solvents. Backgrounds were cleaner and evaporation time was shorter for the chloroform only than for the chloroform/methanol (9:1). The NPD gave sensitivity more than 10-20 times higher than that of FID. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/1639282/Rapid_isolation_with_Sep_Pak_C18_cartridges_and_capillary_gas_chromatography_of_triazine_herbicides_in_human_body_fluids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0379-0738(92)90160-X DB - PRIME DP - Unbound Medicine ER -
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