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Peroxisome proliferator-activated receptor-alpha and -gamma mRNA levels are reduced in chronic hepatitis C with steatosis and genotype 3 infection.
Aliment Pharmacol Ther. 2006 Jan 01; 23(1):107-14.AP

Abstract

BACKGROUND

Steatosis in chronic hepatitis C is associated with inflammation and accelerated fibrogenesis.

AIM

To assess the contribution of peroxisome proliferator-activated receptor-alpha and -gamma to the pathogenesis of hepatitis C virus associated steatosis is unknown.

METHODS

We measured peroxisome proliferator-activated receptor (PPAR)-alpha and -gamma mRNA by quantitative polymerase chain reaction in liver biopsies of 35 genotype 1 and 22 genotype 3 infected patients and in Huh7 cells expressing hepatitis C virus 1b or 3a core protein.

RESULTS

PPAR-alpha mRNA was significantly reduced in livers of patients with genotype 3 compared with genotype 1. Steatosis was associated to a decreased expression of PPAR-alpha in genotype 1, but not in genotype 3. PPAR-gamma expression was significantly lower in genotype 3 compared with genotype 1 and steatosis was associated to decreased levels of PPAR-gamma, but only in genotype 1. There was no significant relationship between PPARs mRNA levels and liver activity or fibrosis. Expression of the hepatitis C virus 3a core protein was associated with an increase in triglyceride accumulation and with a significant reduction of PPAR-gamma mRNA compared with hepatitis C virus 1b.

CONCLUSIONS

The presence of steatosis and hepatitis C virus genotype 3 are both associated with a significant down-regulation of PPARs. These receptors, and also additional factors, seem to play a role in the pathogenesis of hepatitis C virus-associated steatosis.

Authors+Show Affiliations

Services de Gastroentérologie et d'Hépatologie, Hopitaux Universitaires, Geneva, Switzerland. andrea.degottardi@medcli.unige.chNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16393287

Citation

de Gottardi, A, et al. "Peroxisome Proliferator-activated Receptor-alpha and -gamma mRNA Levels Are Reduced in Chronic Hepatitis C With Steatosis and Genotype 3 Infection." Alimentary Pharmacology & Therapeutics, vol. 23, no. 1, 2006, pp. 107-14.
de Gottardi A, Pazienza V, Pugnale P, et al. Peroxisome proliferator-activated receptor-alpha and -gamma mRNA levels are reduced in chronic hepatitis C with steatosis and genotype 3 infection. Aliment Pharmacol Ther. 2006;23(1):107-14.
de Gottardi, A., Pazienza, V., Pugnale, P., Bruttin, F., Rubbia-Brandt, L., Juge-Aubry, C. E., Meier, C. A., Hadengue, A., & Negro, F. (2006). Peroxisome proliferator-activated receptor-alpha and -gamma mRNA levels are reduced in chronic hepatitis C with steatosis and genotype 3 infection. Alimentary Pharmacology & Therapeutics, 23(1), 107-14.
de Gottardi A, et al. Peroxisome Proliferator-activated Receptor-alpha and -gamma mRNA Levels Are Reduced in Chronic Hepatitis C With Steatosis and Genotype 3 Infection. Aliment Pharmacol Ther. 2006 Jan 1;23(1):107-14. PubMed PMID: 16393287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peroxisome proliferator-activated receptor-alpha and -gamma mRNA levels are reduced in chronic hepatitis C with steatosis and genotype 3 infection. AU - de Gottardi,A, AU - Pazienza,V, AU - Pugnale,P, AU - Bruttin,F, AU - Rubbia-Brandt,L, AU - Juge-Aubry,C E, AU - Meier,C A, AU - Hadengue,A, AU - Negro,F, PY - 2006/1/6/pubmed PY - 2006/5/4/medline PY - 2006/1/6/entrez SP - 107 EP - 14 JF - Alimentary pharmacology & therapeutics JO - Aliment Pharmacol Ther VL - 23 IS - 1 N2 - BACKGROUND: Steatosis in chronic hepatitis C is associated with inflammation and accelerated fibrogenesis. AIM: To assess the contribution of peroxisome proliferator-activated receptor-alpha and -gamma to the pathogenesis of hepatitis C virus associated steatosis is unknown. METHODS: We measured peroxisome proliferator-activated receptor (PPAR)-alpha and -gamma mRNA by quantitative polymerase chain reaction in liver biopsies of 35 genotype 1 and 22 genotype 3 infected patients and in Huh7 cells expressing hepatitis C virus 1b or 3a core protein. RESULTS: PPAR-alpha mRNA was significantly reduced in livers of patients with genotype 3 compared with genotype 1. Steatosis was associated to a decreased expression of PPAR-alpha in genotype 1, but not in genotype 3. PPAR-gamma expression was significantly lower in genotype 3 compared with genotype 1 and steatosis was associated to decreased levels of PPAR-gamma, but only in genotype 1. There was no significant relationship between PPARs mRNA levels and liver activity or fibrosis. Expression of the hepatitis C virus 3a core protein was associated with an increase in triglyceride accumulation and with a significant reduction of PPAR-gamma mRNA compared with hepatitis C virus 1b. CONCLUSIONS: The presence of steatosis and hepatitis C virus genotype 3 are both associated with a significant down-regulation of PPARs. These receptors, and also additional factors, seem to play a role in the pathogenesis of hepatitis C virus-associated steatosis. SN - 0269-2813 UR - https://www.unboundmedicine.com/medline/citation/16393287/Peroxisome_proliferator_activated_receptor_alpha_and__gamma_mRNA_levels_are_reduced_in_chronic_hepatitis_C_with_steatosis_and_genotype_3_infection_ L2 - https://doi.org/10.1111/j.1365-2036.2006.02729.x DB - PRIME DP - Unbound Medicine ER -