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Aurothiomalate and hydroxychloroquine inhibit nitric oxide production in chondrocytes and in human osteoarthritic cartilage.
Scand J Rheumatol. 2005 Nov-Dec; 34(6):475-9.SJ

Abstract

OBJECTIVES

Nitric oxide (NO) is a destructive mediator produced by activated chondrocytes. The aim of the present study was to investigate the effect of disease-modifying anti-rheumatic drugs (DMARDs) on interleukin-1beta (IL-1beta)-induced NO production in chondrocyte cultures, and in human osteoarthritic cartilage.

RESULTS

Aurothiomalate, hydroxychloroquine, methotrexate and leflunomide inhibited IL-1beta-induced inducible NO synthase (iNOS) expression and NO production in immortalized H4 chondrocytes, while penicillamine and sulfasalazine had no effect. This can be explained by the fact that the four effective DMARDs also suppressed IL-1beta-induced activation of nuclear factor kappa B (NF-kappaB), which is a crucial transcription factor for iNOS. Aurothiomalate and hydroxychloroquine also inhibited IL-1beta-induced NO production in OA cartilage whereas methotrexate and leflunomide had no effect.

CONCLUSION

Aurothiomalate and hydroxychloroquine suppressed IL-1beta-induced NO production in chondrocyte cultures and in OA cartilage. The results suggest an additional anti-inflammatory mechanism for aurothiomalate and hydroxychloroquine and indicates their possible therapeutic value in the treatment of osteoarthritis (OA).

Authors+Show Affiliations

The Immunopharmacological Research Group, Medical School, University of Tampere, Tampere University Hospital, Finland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16393772

Citation

Vuolteenaho, K, et al. "Aurothiomalate and Hydroxychloroquine Inhibit Nitric Oxide Production in Chondrocytes and in Human Osteoarthritic Cartilage." Scandinavian Journal of Rheumatology, vol. 34, no. 6, 2005, pp. 475-9.
Vuolteenaho K, Kujala P, Moilanen T, et al. Aurothiomalate and hydroxychloroquine inhibit nitric oxide production in chondrocytes and in human osteoarthritic cartilage. Scand J Rheumatol. 2005;34(6):475-9.
Vuolteenaho, K., Kujala, P., Moilanen, T., & Moilanen, E. (2005). Aurothiomalate and hydroxychloroquine inhibit nitric oxide production in chondrocytes and in human osteoarthritic cartilage. Scandinavian Journal of Rheumatology, 34(6), 475-9.
Vuolteenaho K, et al. Aurothiomalate and Hydroxychloroquine Inhibit Nitric Oxide Production in Chondrocytes and in Human Osteoarthritic Cartilage. Scand J Rheumatol. 2005 Nov-Dec;34(6):475-9. PubMed PMID: 16393772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aurothiomalate and hydroxychloroquine inhibit nitric oxide production in chondrocytes and in human osteoarthritic cartilage. AU - Vuolteenaho,K, AU - Kujala,P, AU - Moilanen,T, AU - Moilanen,E, PY - 2006/1/6/pubmed PY - 2006/3/29/medline PY - 2006/1/6/entrez SP - 475 EP - 9 JF - Scandinavian journal of rheumatology JO - Scand J Rheumatol VL - 34 IS - 6 N2 - OBJECTIVES: Nitric oxide (NO) is a destructive mediator produced by activated chondrocytes. The aim of the present study was to investigate the effect of disease-modifying anti-rheumatic drugs (DMARDs) on interleukin-1beta (IL-1beta)-induced NO production in chondrocyte cultures, and in human osteoarthritic cartilage. RESULTS: Aurothiomalate, hydroxychloroquine, methotrexate and leflunomide inhibited IL-1beta-induced inducible NO synthase (iNOS) expression and NO production in immortalized H4 chondrocytes, while penicillamine and sulfasalazine had no effect. This can be explained by the fact that the four effective DMARDs also suppressed IL-1beta-induced activation of nuclear factor kappa B (NF-kappaB), which is a crucial transcription factor for iNOS. Aurothiomalate and hydroxychloroquine also inhibited IL-1beta-induced NO production in OA cartilage whereas methotrexate and leflunomide had no effect. CONCLUSION: Aurothiomalate and hydroxychloroquine suppressed IL-1beta-induced NO production in chondrocyte cultures and in OA cartilage. The results suggest an additional anti-inflammatory mechanism for aurothiomalate and hydroxychloroquine and indicates their possible therapeutic value in the treatment of osteoarthritis (OA). SN - 0300-9742 UR - https://www.unboundmedicine.com/medline/citation/16393772/Aurothiomalate_and_hydroxychloroquine_inhibit_nitric_oxide_production_in_chondrocytes_and_in_human_osteoarthritic_cartilage_ L2 - https://www.tandfonline.com/doi/full/10.1080/03009740510026797 DB - PRIME DP - Unbound Medicine ER -