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Virucidal activity of a beta-triketone-rich essential oil of Leptospermum scoparium (manuka oil) against HSV-1 and HSV-2 in cell culture.
Planta Med. 2005 Dec; 71(12):1123-7.PM

Abstract

The inhibitory activity of manuka oil against Herpes simplex virus type 1 (HSV-1) and Herpes simplex virus type 2 (HSV-2) was tested in vitro on RC-37 cells (monkey kidney cells) using a plaque reduction assay. In order to determine the mode of antiviral action of the essential oil, manuka oil was added at different times to the cells or viruses during the infection cycle. Both HSV types were significantly inhibited when the viruses were pretreated with manuka oil 1 h prior to cell infection. At non-cytotoxic concentrations of the essential oil, plaque formation was significantly reduced by 99.5 % and 98.9 % for HSV-1 and HSV-2, respectively. The 50 % inhibitory concentration (IC (50)) of manuka oil for virus plaque formation was determined at 0.0001 % v/v (= 0.96 microg/mL) and 0.00006 % v/v (= 0.58 microg/mL) for HSV-1 and HSV-2, respectively. On the other hand, pretreatment of host cells with the essential oil before viral infection did not affect plaque formation. After virus penetration into the host cells only replication of HSV-1 particle was significantly inhibited to about 41 % by manuka oil. Flavesone and leptospermone, two characteristic ss-triketones of manuka oil, inhibited the virulence of HSV-1 in the same manner as the essential oil itself. When added at non-cytotoxic concentrations to the virus 1 h prior to cell infection, plaque formation was reduced by 99.1 % and 79.7 % for flavesone and leptospermone, respectively.

Authors+Show Affiliations

Reichling J
Institute of Pharmacy and Molecular Biotechnology, Department of Biology, University of Heidelberg, Heidelberg, Germany. juergen.reichling@urz.uni-heidelberg.de
Koch C
No affiliation info available
Stahl-Biskup E
No affiliation info available
Sojka C
No affiliation info available
Schnitzler P
No affiliation info available

MeSH

AnimalsAntiviral AgentsCell LineChlorocebus aethiopsHerpesvirus 1, HumanHerpesvirus 2, HumanInhibitory Concentration 50LeptospermumMolecular StructureOils, VolatilePhloroglucinolPhytotherapyPlant OilsVirus Replication

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16395648

Citation

Reichling, Jürgen, et al. "Virucidal Activity of a Beta-triketone-rich Essential Oil of Leptospermum Scoparium (manuka Oil) Against HSV-1 and HSV-2 in Cell Culture." Planta Medica, vol. 71, no. 12, 2005, pp. 1123-7.
Reichling J, Koch C, Stahl-Biskup E, et al. Virucidal activity of a beta-triketone-rich essential oil of Leptospermum scoparium (manuka oil) against HSV-1 and HSV-2 in cell culture. Planta Med. 2005;71(12):1123-7.
Reichling, J., Koch, C., Stahl-Biskup, E., Sojka, C., & Schnitzler, P. (2005). Virucidal activity of a beta-triketone-rich essential oil of Leptospermum scoparium (manuka oil) against HSV-1 and HSV-2 in cell culture. Planta Medica, 71(12), 1123-7.
Reichling J, et al. Virucidal Activity of a Beta-triketone-rich Essential Oil of Leptospermum Scoparium (manuka Oil) Against HSV-1 and HSV-2 in Cell Culture. Planta Med. 2005;71(12):1123-7. PubMed PMID: 16395648.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virucidal activity of a beta-triketone-rich essential oil of Leptospermum scoparium (manuka oil) against HSV-1 and HSV-2 in cell culture. AU - Reichling,Jürgen, AU - Koch,Christine, AU - Stahl-Biskup,Elisabeth, AU - Sojka,Cornelia, AU - Schnitzler,Paul, PY - 2006/1/6/pubmed PY - 2006/3/11/medline PY - 2006/1/6/entrez SP - 1123 EP - 7 JF - Planta medica JO - Planta Med VL - 71 IS - 12 N2 - The inhibitory activity of manuka oil against Herpes simplex virus type 1 (HSV-1) and Herpes simplex virus type 2 (HSV-2) was tested in vitro on RC-37 cells (monkey kidney cells) using a plaque reduction assay. In order to determine the mode of antiviral action of the essential oil, manuka oil was added at different times to the cells or viruses during the infection cycle. Both HSV types were significantly inhibited when the viruses were pretreated with manuka oil 1 h prior to cell infection. At non-cytotoxic concentrations of the essential oil, plaque formation was significantly reduced by 99.5 % and 98.9 % for HSV-1 and HSV-2, respectively. The 50 % inhibitory concentration (IC (50)) of manuka oil for virus plaque formation was determined at 0.0001 % v/v (= 0.96 microg/mL) and 0.00006 % v/v (= 0.58 microg/mL) for HSV-1 and HSV-2, respectively. On the other hand, pretreatment of host cells with the essential oil before viral infection did not affect plaque formation. After virus penetration into the host cells only replication of HSV-1 particle was significantly inhibited to about 41 % by manuka oil. Flavesone and leptospermone, two characteristic ss-triketones of manuka oil, inhibited the virulence of HSV-1 in the same manner as the essential oil itself. When added at non-cytotoxic concentrations to the virus 1 h prior to cell infection, plaque formation was reduced by 99.1 % and 79.7 % for flavesone and leptospermone, respectively. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/16395648/Virucidal_activity_of_a_beta_triketone_rich_essential_oil_of_Leptospermum_scoparium__manuka_oil__against_HSV_1_and_HSV_2_in_cell_culture_ DB - PRIME DP - Unbound Medicine ER -