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Regulation of HLA class II antigen expression on cultured corneal epithelium by interferon-gamma.
Invest Ophthalmol Vis Sci. 1992 Aug; 33(9):2714-21.IO

Abstract

The effect of recombinant human interferon gamma (IFN-gamma) on the induction of HLA class II (HLA-DR, -DP, -DQ) antigen expression on human corneal epithelial (HCE) cells was examined in different stages of culture. Primary cultures were established with limbal explants without endothelium. HCE cells in Stage 1 and Stage 2, with cells negative and positive for the 64K corneal keratin (the marker for advanced corneal epithelial differentiation), respectively, were prepared. HCE cells in both stages were treated with IFN-gamma at a concentration of 0 to 1000 U/ml for two to six days and were stained by the avidin-biotin peroxidase complex method. Class II antigens were not detected on HCE cells in either stage without IFN-gamma treatment. IFN-gamma induced three class II antigens on HCE cells in both stages in a dose- and time-dependent manner but at different levels for each antigen (DR greater than DP greater than DQ). In addition, DQ expression was related to cell differentiation, with DQ extremely rare at Stage 1 and more frequent at Stage 2 (5% vs. 20%). These findings indicate that the induction of class II antigens on HCE cells may be regulated by IFN-gamma independently for each of the antigens and that DQ induction may depend upon the differentiation of HCE cells in culture.

Authors+Show Affiliations

Department of Ophthalmology, Eye and Ear Institute of Pittsburgh, PA 15213.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1639617

Citation

Iwata, M, et al. "Regulation of HLA Class II Antigen Expression On Cultured Corneal Epithelium By Interferon-gamma." Investigative Ophthalmology & Visual Science, vol. 33, no. 9, 1992, pp. 2714-21.
Iwata M, Kiritoshi A, Roat MI, et al. Regulation of HLA class II antigen expression on cultured corneal epithelium by interferon-gamma. Invest Ophthalmol Vis Sci. 1992;33(9):2714-21.
Iwata, M., Kiritoshi, A., Roat, M. I., Yagihashi, A., & Thoft, R. A. (1992). Regulation of HLA class II antigen expression on cultured corneal epithelium by interferon-gamma. Investigative Ophthalmology & Visual Science, 33(9), 2714-21.
Iwata M, et al. Regulation of HLA Class II Antigen Expression On Cultured Corneal Epithelium By Interferon-gamma. Invest Ophthalmol Vis Sci. 1992;33(9):2714-21. PubMed PMID: 1639617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of HLA class II antigen expression on cultured corneal epithelium by interferon-gamma. AU - Iwata,M, AU - Kiritoshi,A, AU - Roat,M I, AU - Yagihashi,A, AU - Thoft,R A, PY - 1992/8/1/pubmed PY - 1992/8/1/medline PY - 1992/8/1/entrez SP - 2714 EP - 21 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 33 IS - 9 N2 - The effect of recombinant human interferon gamma (IFN-gamma) on the induction of HLA class II (HLA-DR, -DP, -DQ) antigen expression on human corneal epithelial (HCE) cells was examined in different stages of culture. Primary cultures were established with limbal explants without endothelium. HCE cells in Stage 1 and Stage 2, with cells negative and positive for the 64K corneal keratin (the marker for advanced corneal epithelial differentiation), respectively, were prepared. HCE cells in both stages were treated with IFN-gamma at a concentration of 0 to 1000 U/ml for two to six days and were stained by the avidin-biotin peroxidase complex method. Class II antigens were not detected on HCE cells in either stage without IFN-gamma treatment. IFN-gamma induced three class II antigens on HCE cells in both stages in a dose- and time-dependent manner but at different levels for each antigen (DR greater than DP greater than DQ). In addition, DQ expression was related to cell differentiation, with DQ extremely rare at Stage 1 and more frequent at Stage 2 (5% vs. 20%). These findings indicate that the induction of class II antigens on HCE cells may be regulated by IFN-gamma independently for each of the antigens and that DQ induction may depend upon the differentiation of HCE cells in culture. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/1639617/Regulation_of_HLA_class_II_antigen_expression_on_cultured_corneal_epithelium_by_interferon_gamma_ L2 - https://iovs.arvojournals.org/article.aspx?volume=33&issue=9&page=2714 DB - PRIME DP - Unbound Medicine ER -